Title :
A Novel PDMS Microfluidic Spotter for Fabrication of Protein Chips and Microarrays
Author :
Chang-Yen, David A. ; Myszka, David G. ; Gale, Bruce K.
Author_Institution :
Utah State Center of Excellence for Biomed. Microfluidics, Utah Univ., Salt Lake City, UT
Abstract :
A novel polydimethyl siloxane (PDMS) microfluidic spotter system has been developed for the patterning of surface microarrays that require individually addressing each spot area and high probe density. Microfluidic channels are used to address each spot region, and large spot arrays can be addressed in parallel. Fluorescence intensity measurement of dye-spotted samples compared to control and pipetted drops demonstrated a minimum of three-fold increase in dye surface density compared to pin-spotted dyes. Surface plasmon resonance (SPR) measurement of protein-spotted samples as compared to pin-spotted samples demonstrated an 86-fold increase in protein surface concentration. The spotting system has been applied successfully to protein microarrays for SPR applications, in both a 12-spot linear and 48-spot two-dimensional (2-D) array format. This novel spotter system can be applied to the production of high-throughput arrays in the fields of genomics, proteomics, immunoassays, and fluorescence or luminescence assays
Keywords :
dyes; fluorescence spectroscopy; microfluidics; proteins; surface plasmon resonance; 2D array format; PDMS; dye surface density; fluorescence intensity measurement; microfluidic channels; microfluidic spotter; pin-spotted dyes; pin-spotted samples; polydimethyl siloxane; protein chips; protein microarrays; protein surface concentration; protein-spotted samples; surface microarrays; surface plasmon resonance measurement; Density measurement; Fabrication; Fluorescence; Microfluidics; Plasmons; Probes; Production systems; Proteins; Resonance; Two dimensional displays; Microarray; microfluidic; polydimethyl siloxane (PDMS); spotter;
Journal_Title :
Microelectromechanical Systems, Journal of
DOI :
10.1109/JMEMS.2006.880289
