Title :
Morphological study of Saccharomyces cerevisiae cells treated with magnetic fluid
Author :
Azevedo, R.B. ; Silva, L.P. ; Lemos, A.P.C. ; Báo, S.N. ; Lacava, Z.G.M. ; Safarik, I. ; Safarikova, M. ; Morais, P.C.
Author_Institution :
Inst. de Ciencias Biologicas, Univ. de Brasilia, Brazil
Abstract :
This paper shows that Saccharomyces cerevisiae efficiently interact with magnetite-based ionic magnetic fluid, leading to the formation of magnetically labeled cells which could be easily separated from the system using an appropriated field-gradient-based magnetic separator. Scanning and transmission electron microscopies were used to investigate the interaction of Saccharomyces cerevisiae cells with magnetite nanoparticles. The high-resolution microscopy data suggested that particle incorporation occurs via an active process. Further, the microscopy data shows that the particles did not reach the cell cytoplasm, staying in the periplasmatic space.
Keywords :
biological effects of fields; biomagnetism; cellular biophysics; iron compounds; magnetic fluids; magnetic separation; nanoparticles; scanning electron microscopy; transmission electron microscopy; Fe3O4; Saccharomyces cerevisiae; field gradient magnetic separator; ionic magnetic fluid; magnetic labeling; magnetite nanoparticles; scanning electron microscopy; transmission electron microscopy; yeast cell morphology; Fungi; Lead; Magnetic liquids; Magnetic materials; Magnetic separation; Nanoparticles; Scanning electron microscopy; Space technology; Transmission electron microscopy; Wastewater;
Journal_Title :
Magnetics, IEEE Transactions on
DOI :
10.1109/TMAG.2003.815547