Title :
Resonance light scattering (RLS) detection of nanoparticle separations in a microelectrical field-flow fractionation system
Author :
Graff, Mason ; Frazier, A. Bruno
Author_Institution :
Sch. of Electr. & Comput. Eng., Georgia Inst. of Technol., USA
Abstract :
In this study, resonance light scattering (RLS) is demonstrated as a detection methodology for real-time visualization of the electrical field-flow fractionation process. On-column RLS was used to monitor the fractionation of a bolus sample containing 40-, 60-, and 80-nm-diameter RLS Particles into individual particles and bands of particles. Optical detection using the RLS system was correlated to an on-chip electrical conductivity detector. Particle concentrations as low as 1.08×10-12 M (1 RLS Particle/1.54 pL) were detected using both the optical and electrical detection systems.
Keywords :
electrical conductivity; fractionation; light scattering; microfluidics; micromachining; nanoparticles; particle size; 40 nm; 60 nm; 80 nm; electrical conductivity detector; electrical detection systems; microelectrical field-flow fractionation system; microfluidics; nanoparticle detection; optical detection; particle concentrations; particles size; resonance light scattering; separations; Biomedical optical imaging; Electromagnetic scattering; Field-flow fractionation; Light scattering; Mie scattering; Nanobioscience; Optical detectors; Optical scattering; Rayleigh scattering; Resonance light scattering; Conductivity measurement; micromachining, separation; resonance light scattering;
Journal_Title :
Nanotechnology, IEEE Transactions on
DOI :
10.1109/TNANO.2005.858596
