Title :
Stabilizing effect of artificial stabilizers for binding activity of QCM immunosensors
Author :
Park, Jong-Won ; Kurosawa, Shigeru ; Aizawa, Hidenobu ; Wakida, Shin-ichi ; Yamada, Satoshi ; Ishihara, Kazuhiko
Author_Institution :
Nat. Inst. of Adv. Ind. Sci. & Technol., Japan
Abstract :
We compare stabilizing effects of artificial and natural stabilizers for maintenance of binding activity of immobilized antibody on the gold surface of quartz crystal microbalance (QCM). Binding activity between immobilizing antibody and specific antigen was measured and considered as a calculated frequency shift with QCM-immunosensor after temperature increasing and long-term storage. According to the experimental results, one stabilizer among eight samples shows the best stabilizing effect for QCM-immunosensor.
Keywords :
biosensors; crystal resonators; microbalances; quartz; Au; SiO/sub 2/; artificial stabilizer; binding activity; frequency shift; gold surface; immobilized antibody; immunosensor; natural stabilizer; quartz crystal microbalance; specific antigen; Chemicals; Electrodes; Frequency measurement; Gold; Immune system; Laboratories; Pollution measurement; Polymers; Proteins; Temperature; Adsorption; Antigen-Antibody Complex; Biosensing Techniques; Coated Materials, Biocompatible; Drug Stability; Electrochemistry; Equipment Failure Analysis; Immunoassay; Microchemistry; Protein Binding; Quartz; Temperature;
Journal_Title :
Ultrasonics, Ferroelectrics, and Frequency Control, IEEE Transactions on
DOI :
10.1109/TUFFC.2003.1244737
