Title :
Transfection by electroporation: cell gene transfer using electrical impulses, a new process in blood cancer research
Author :
Jamieson, R.D. ; Bodger, M.P. ; Bodger, P.S. ; Baran, D.
Author_Institution :
Electricorp, New Plymouth, New Zealand
Abstract :
Isolated genetic material can be transferred into a host cell through its membrane by the application of an appropriate electrical impulse to the cell. The authors describe a three-stage impulse generator designed to transfect viral genes into human blood cells, to aid scientists in their research into blood cancers by immortalizing the cells. Trials on samples from a terminal cancer patient resulted in successful electroporation of an antibiotic-resistant gene.<>
Keywords :
bioelectric phenomena; biological techniques and instruments; biomedical electronics; biomedical equipment; biomembrane transport; blood; cellular biophysics; pulse generators; antibiotic-resistant gene; blood cancer; cell gene transfer; electrical impulses; electroporation; host cell; human blood cells; membrane; research; three-stage impulse generator; transfection; viral genes; Bioelectric phenomena; Biological cells; Biomedical equipment; Biomembranes; Biophysics; Blood; Pulse generation;
Journal_Title :
Physical Science, Measurement and Instrumentation, Management and Education, IEE Proceedings A
