DocumentCode :
839926
Title :
Preparation of nano-sized particles from collagen II by a high-voltage electrostatic field system
Author :
Chang, S.J. ; Niu, G.C.-C. ; Kuo, S.M. ; Ho, C.C. ; Bair, M.S.
Author_Institution :
Dept. of Biomed. Eng., I-SHOU Univ., Taiwan
Volume :
153
Issue :
1
fYear :
2006
Firstpage :
1
Lastpage :
6
Abstract :
The pilot study describes a novel method for preparing nano-sized particles from collagen II using a high-voltage electrostatic field system. Observations from transmission electron microscopy showed that, in one of the cases, the nano-sized collagen II particles exhibited good sphericity, and the particles were in the range of 23.3±1.7 nm in diameter at the experimental setting of 3 kV cm-1, for a 3 h treatment period and at 25°C (with a collagen concentration of 0.2 mg ml-1). When the treatment temperature increased to 30°C, the collagen II began to lose the tendency to form individually separated spherically shaped nano-particles. Moreover, a fibrous structure of collagen II was formed instead of a nano-particle shape at the temperature of 37°C. This result is probably contributed to by an entropy-driven process that is termed fibrillogenesis, a larger force causing the collagen molecules to self-assemble and then form collagen fibrils. It is interesting to note that this is practically the first attempt to produce nano-particles directly from collagen II solution under the treatment of a high-voltage electrostatic field, together with a set of working parameters for the collagen concentration and low-temperature setting.
Keywords :
bioelectric potentials; biomedical materials; entropy; molecular biophysics; nanoparticles; nanotechnology; proteins; self-assembly; transmission electron microscopy; 21.6 to 25.0 nm; 25 degC; 3 h; 30 degC; collagen II; entropy-driven process; fibrillogenesis; high-voltage electrostatic field system; individually separated spherically shaped nanoparticles; nanosized particles; transmission electron microscopy;
fLanguage :
English
Journal_Title :
Nanobiotechnology, IEE Proceedings -
Publisher :
iet
ISSN :
1478-1581
Type :
jour
DOI :
10.1049/ip-nbt:20050037
Filename :
1597600
Link To Document :
بازگشت