Blood typing chip using human whole blood sample

Blood typing is a critical test for blood transfusion and many medical procedures. Described is a disposable microfluidic blood typing chip with a simple fabrication process. The chip allows one to use human whole blood directly so that the operation sequence of a manual polybrene technique for blood typing can be conducted automatically. In addition, the determination of the extent of red blood cell agglutination can be analysed by software. The feasibility of this chip was demonstrated by ABO blood typing tests using antibody screening cells and freshly drawn venous blood samples. From the pixel count of agglutination, the chip provided correct blood typing results. This proposed microfluidic chip has the merits of smaller consumption volumes of reagents. Moreover, the simple fabrication process of the chip results in low fabrication cost.