The first part of this paper is concerned with differentiation of the Karhunen-Loève expansion of a stochastic process. In particular, we establish that the expansion series can be differentiated term by term while retaining the same sense of convergence, ff the covariance 

 has a continuous second partial derivative and the sample function 

 is almost surely differentiable. The result can be generalized to the case of higher-order differentiation. Namely, if 

 is continuous and 

 has the 

 th derivative 

 almost surely, then the series can be differentiated term by term 

 times, and the resultant series converges in the stochastic mean to 

 uniformly in 

 . In the second half, the above result is applied to the problem of optimum reception of binary signals in Gaussian noise. Suppose the binary sure signals are 

 and 

 and the noise covariance is 

 . Then we prove the well-known conjecture that the optimum receiver correlates the observable waveform with the solution 

 of the integral equation 

 even if the solution contains 

 -functions and their derivatives. This result can be generalized to the case of 

 -ary sure signals.