DocumentCode :
911589
Title :
CCD-based synchrotron x-ray detector for protein crystallograph-performance projected from an experiment
Author :
Strauss, Michael G. ; Naday, Istvan ; Sherman, Irvin S. ; Kraimer, Martin R. ; Westbrook, Edwin M.
Author_Institution :
Argonne National Laboratory Argonne, Illinois 60439
Volume :
34
Issue :
1
fYear :
1987
Firstpage :
389
Lastpage :
395
Abstract :
The intense x radiation from a synchrotron source could, with a suitable detector, provide a complete set of diffraction images from a protein crystal before the crystal is damaged by radiation (2-3 min). An area detector consisting of a 40 mm dia. x-ray fluorescing phosphor, coupled with an image intensifier and lens to a CCD image sensor, was developed to determine the effectiveness of such a detector in protein crystallography. The detector was used in an experiment with a rotating anode x-ray generator. Diffraction patterns from a lysozyme crystal obtained with this detector are compared to those obtained with film. The two images appear to be virtually identical. The flux of 10/4 x-ray photons/s was observed on the detector at the rotating anode generator. At the 6-GeV synchrotron being designed at Argonne, the flux on an 80×80 mm2 detector is expected to be ≫ 109 photons/s. The projected design of such a synchrotron detector shows that a diffraction-peak count ≫ 106 could be obtained in ˜0.5 s. With an additional ˜0.5 s readout time of a 512×512 pixel CCD, the data acquisition time per frame would be ˜1 s so that ninety 1o diffraction images could be obtained, with approximately 1% precision, in less than 3 min.
Keywords :
Anodes; Crystallography; Phosphors; Proteins; Radiation detectors; Synchrotron radiation; X-ray detection; X-ray detectors; X-ray diffraction; X-ray imaging;
fLanguage :
English
Journal_Title :
Nuclear Science, IEEE Transactions on
Publisher :
ieee
ISSN :
0018-9499
Type :
jour
DOI :
10.1109/TNS.1987.4337369
Filename :
4337369
Link To Document :
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