The Use of Television and Scanning Techniques for Ultraviolet Irradiation Studies of Living Cells

The Ultraviolet Flying-Spot Television Microscope is a new tool for the study of living cells. The original development of the technique has been described by the authors elsewhere. One of the major uses of this technique is the study of ultraviolet irradiation damage in living cells. Recent advances in the technique now enable one to perform experiments designed to elucidate the relationship between total cellular damage and cellular component damage. This paper describes the methods for the simultaneous utilization of two wavelengths of light for the illumination of different portions of a single living cell. This technical advance now makes it possible to produce ultraviolet irradiation damage in any area of the cell while illuminating the remainder of the cell with nondamaging visible light purely for image production purposes.