The cation distribution of single crystal manganese ferrites Mn
1-yFe
y[Mn
yFe
2-y]O
4with

was modified by appropriate heat treatment at temperatures 300-600°C. With these samples the Curie temperature, the magnetocrystalline anisotropy, the spontaneous magnetization and the differential susceptibility at high magnetic fields were studied. The experiments enabled us to determine the magnitude of the molecular field coefficient N
Mn(A)-Fe(B)= 89 and to estimate the main part of the contribution to the magnetocrystalline anisotropy in MnFe
2O
4which was found to come from Fe
3+in octahedral sites. High field susceptibility measurements confirmed the applicability of the noncolinear arrangement of magnetic moments in MnFe
2O
4which supposes that Mn(B) spins make an angle of 53° with the direction of the total magnetization.