پديد آورندگان :
فلاحي، فرامرز دانشگاه شاهد - گروه قلب و عروق , روغني، مهرداد دانشگاه شاهد - گروه فيزيولوژي و مركز تحقيقات گياهان دارويي , خليلي زاده، مجيد دانشگاه شاهد
كليدواژه :
ديابت قندي , پاسخ انقباضي , آئورت , سير وحشي
چكيده فارسي :
مقدمه: با توجه به وجود شواهدي مبني بر اثر ضد ديابتي سير وحشي، اثر مصرف خوراكي اين گياه بر پاسخ انقباضي آئورت سينه‌اي در موش ديابتي مورد بررسي قرار گرفت. روش كار: در اين مطالعه تجربي- آزمايشگاهي 40 موش صحرايي نر به 5 گروه كنترل، كنترل تحت تيمار با سير وحشي، ديابتي، ديابتي تحت درمان با گياه و تحت تيمار با گليبن كلاميد تقسيم‌بندي شدند. براي ديابتي كردن موش‌ها از استرپتوزوتوسين به ميزان 60 ميلي‌گرم بر كيلوگرم استفاده شد. دو گروه تحت تيمار با گياه نيز پودر اين گياه مخلوط شده با غذاي استاندارد موش را با نسبت وزني 1 درصد دريافت نمودند. ميزان گلوكز سرم قبل از انجام كار و در هفته‌هاي 4 و 8 اندازه‌گيري شد. در پايان، پاسخ انقباضي حلقه‌هاي آئورت سينه‌اي به كلرور پتاسيم و نور آدرنالين مورد بررسي قرار گرفت. نتايج: ميزان گلوكز سرم در گروه ديابتي تحت تيمار با گياه در هفته‌هاي 4 و 8 به طور معني‌دار كمتر از گروه ديابتي بود (به ترتيب 01/0p< و 005/0p<). به علاوه حداكثر پاسخ انقباضي آئورت سينه‌اي به نورآدرنالين در گروه ديابتي تحت درمان با سير وحشي به طور معني‌دار كمتر از گروه ديابتي درمان نشده بود(05/0p<) و چنين كاهش معني‌داري در مورد پاسخ انقباضي به كلرور پتاسيم مشاهده نشد. نتيجه گيري: مصرف خوراكي سير وحشي به مدت 2 ماه داراي اثر هيپوگليسميك در حد متوسط بوده و در كاهش دادن پاسخ انقباضي سيستم عروقي در موش‌هاي ديابتي موثر است.
چكيده لاتين :
Background: Considering some evidence on anti-diabetic potential of Allium ursinum (AU) , this study was conducted to evaluate the effect of oral administration of AU on contractile responsiveness of thoracic aorta in diabetic rats.
Materials and Methods: In this experimental study, 40 male Wistar rats were divided into control, AU-treated control, diabetic, glibenclamide-treated, and AU-treated diabetic groups. For inducing diabetes, streptozotcin (STZ) was administered (60 mg/Kg). AU-treated group received AU mixed with standard pelleted food at a weight ratio of 1% for 2 months. Serum glucose level was measured at weeks 4 and 8. Eventually, contractile responsiveness of thoracic aortic rings to KCl and noradrenaline (NA) was evaluated .
Results: Serum glucose level, at weeks 4 and 8, in the AU-treated diabetic group was significantly lower than that in the diabetics group (p<0.01 and p<0.005, respectively). In addition, the maximum thoracic aorta contractile responsiveness to NA in the AU-treated diabetic group was significantly less than the diabetic group (p<0.05) however, such a significant reduction was not observed for KCl.
Conclusion: Oral administration of AU for 2 months is of a moderate hypoglycemic effect and attenuates the contractile responsiveness of the vascular system in diabetic rats. Background: Considering some evidence on anti-diabetic potential of Allium ursinum (AU), this study was conducted to evaluate the effect of oral administration of AU on contractile responsiveness of thoracic aorta in diabetic rats. Materials and Methods: In this experimental study, 40 male Wistar rats were divided into control, AU-treated control, diabetic, glibenclamide-treated, and AU-treated diabetic groups. For inducing diabetes, streptozotcin (STZ) was administered (60 mg/Kg). AU-treated group received AU mixed with standard pelleted food at a weight ratio of 1% for 2 months. Serum glucose level was measured at weeks 4 and 8. Eventually, contractile responsiveness of thoracic aortic rings to KCl and noradrenaline (NA) was evaluated. Results: Serum glucose level, at weeks 4 and 8, in the AU-treated diabetic group was significantly lower than that in the diabetics group (p<0.01 and p<0.005, respectively). In addition, the maximum thoracic aorta contractile responsiveness to NA in the AU-treated diabetic group was significantly less than the diabetic group (p<0.05) however, such a significant reduction was not observed for KCl. Conclusion: Oral administration of AU for 2 months is of a moderate hypoglycemic effect and attenuates the contractile responsiveness of the vascular system in diabetic rats.
