ارزيابي كمي و كيفي برخي متابوليت هاي اوليه و ثانويه گياه دارويي برازمبل (Proveskia abrotanoides Karel.) در مراحل فنولوژيكي مختلف

Evaluation of some primary and secondary metabolites of medicinal plant Proveskia abrotanoides Karel. in different phenological stages

ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ ﮐﻤﯽ و ﮐﯿﻔﯽ ﺑﺮﺧﯽ ﻣﺘﺎﺑﻮﻟﯿﺖﻫﺎي اوﻟﯿﻪ و ﺛﺎﻧﻮﯾﻪ ﮔﯿﺎه داروﯾﯽ ﺑﺮازﻣﺒﻞ در ﻣﺮاﺣﻞ ﻓﻨﻮﻟـﻮژﯾﮑﯽ ﻣﺨﺘﻠﻒ اﻧﺪام ﻫﺎي ﮔﯿﺎه در دو ﻣﺮﺣﻠﻪ روﯾﺸﯽ و ﮔﻞ دﻫﯽ از ﻣﻨﻄﻘﻪ روﺳـﺘﺎي واﻣﻨـﺎن واﻗـﻊ در ﺷﻬﺮﺳـﺘﺎن آزادﺷـﻬﺮ ﺟﻤﻊ آوري و ﺧﺸﮏ ﮔﺮدﯾﺪ. در اﯾﻦ ﺗﺤﻘﯿﻖ از ﻋﺼﺎره از ﮐﻞ ﮔﯿﺎه ﺑﺮازﻣﺒﻞ در دو ﻣﺮﺣﻠﻪ روﯾﺸﯽ و ﮔﻠﺪﻫﯽ ﺟﻬﺖ ﺷﻨﺎﺳﺎﯾﯽ و ارزﯾﺎﺑﯽ ﮐﯿﻔﯽ ﺗﺎﻧﻦ ﻫﺎ، ﺳﺎﭘﻮﻧﯿﻦ ﻫﺎ، ﺗﺮﮐﯿﺒﺎت آﻧﺘﻮﺳﯿﺎﻧﯿﻨﯽ و ﺗﺮﭘﻨﻮﺋﯿﺪي، ﻓﻼوون ﻫﺎ و ﻓﻼوﻧﻮﺋﯿﺪﻫﺎ اﺳـﺘﻔﺎده ﺷﺪ. ﻫﻤﭽﻨﯿﻦ ﻣﯿﺰان ﺑﺮﺧﯽ ﻣﺘﺎﺑﻮﻟﯿﺖ ﻫﺎي اوﻟﯿﻪ ﻧﻈﯿﺮ ﮐﺮﺑﻮﻫﯿﺪراتﻫﺎي ﻣﺤﻠﻮل ﻧﺎﻣﺤﻠـﻮل، ﭘـﺮوﻟﯿﻦ و ﻓﻨـﻞ ﮐـﻞ در اﻧﺪام ﻫﺎي رﯾﺸﻪ، ﺳﺎﻗﻪ، ﺑﺮگ و ﮔﻞ ﻣﻮرد ارزﯾﺎﺑﯽ ﮐﻤﯽ ﻗﺮار ﮔﺮﻓﺖ. آﻧﺎﻟﯿﺰ ﮐﯿﻔﯽ ﮐﻞ ﮔﯿﺎه ﺑﺮازﻣﺒـﻞ ﻧﺸـﺎن داد در دو ﻣﺮﺣﻠﻪ روﯾﺸﯽ و ﮔﻠﺪﻫﯽ ﺣﻀﻮر ﺗﺮﮐﯿﺒﺎت ﺳﺎﭘﻮﻧﯿﻨﯽ و اﻧﺘﻮﺳﯿﺎﻧﯿﻨﯽ و ﺗﺎﻧﻦ ﻫﺎ ﻣﺜﺒﺖ ﺑﻮد در ﺣﺎﻟﯽ ﮐﻪ وﺟﻮد ﻓﻼون ﻫﺎ ﺗﻨﻬﺎ در ﻣﺮﺣﻠﻪ روﯾﺸﯽ و ﺗﺮﭘﻨﻮﺋﯿﺪﻫﺎ، ﻓﻼوﻧﯿﺪﻫﺎ ﺗﻨﻬﺎ در ﻣﺮﺣﻠﻪ ﮔﻠﺪﻫﯽ ﻣﺸﺎﻫﺪه ﺷﺪ. آﻧﺎﻟﯿﺰ ﮐﻤﯽ ﻧﯿﺰ ﺑﺎﻻﺗﺮﯾﻦ ﻧﺸﺎﺳﺘﻪ را در ﻣﺮﺣﻠﻪ روﯾﺸﯽ در رﯾﺸﻪ و ﺑﯿﺸﺘﺮﯾﻦ ﮐﺮﺑﻮﻫﯿﺪرات ﻫﺎي ﻣﺤﻠﻮل و ﭘﺮوﻟﯿﻦ و ﺗﺮﮐﯿﺒﺎت ﻓﻨﻠﯽ را در ﺑﺮگ ﻧﺸﺎن داد. در ﻣﺮﺣﻠﻪ ﮔﻠﺪﻫﯽ ﻧﯿﺰ ﻣﯿﺰان ﻧﺸﺎﺳﺘﻪ ﮐﺮﺑﻮﻫﯿﺪرات ﻫﺎي ﻣﺤﻠﻮل ﭘﺮوﻟﯿﻦ و ﻓﻨﻞ ﮐﻞ ﺑﯿﺸﺘﺮﯾﻦ ﻣﻘﺪار را ﺑﻪ ﺧﻮد اﺧﺘﺼﺎص داد. ﺑﻪ ﻃﻮرﮐﻠﯽ ﻧﺘﺎﯾﺞ اﯾﻦ ﺗﺤﻘﯿﻖ ﻧﺸﺎن داد در ﻣﺮﺣﻠﻪ روﯾﺸﯽ ﺻﺮف ﻧﻈﺮ از ﻧﺸﺎﺳﺘﻪ ﺑﺮگ ﻫﺎ ﺣـﺎوي ﺑﯿﺸﺘﺮﯾﻦ ﻗﻨﺪﻫﺎي ﻣﺤﻠﻮل، ﭘﺮوﻟﯿﻦ و ﺗﺮﮐﯿﺒﺎت ﻓﻨﻠﯽ و در ﻣﺮﺣﻠﻪ زاﯾﺸﯽ ﻧﯿﺰ رﯾﺸﻪ از ﺑﯿﺸـﺘﺮﯾﻦ ﺗﺮﮐﯿﺒـﺎت ﯾـﺎد ﺷـﺪه برخوردار بودند

To evaluate the quality and quantity of primary and secondary metabolites at different phenological stages of Brazmbl medicinal plants, plant organs at both vegetative and flowering stages of Vamanan region in Āzādshahr were collected and dried. Whole plant extracts from Proveskia abrotanoides were used in this study at both vegetative and flowering stages to identify and evaluate the quality of tannins, saponins, anthocyanins and terpenoids compounds, flavones, and flavonoids. Also some primary metabolites such as soluble and insoluble carbohydrates, proline and total phenol in roots, stems, leaves, and flowers of plant were evaluated quantitatively. Qualitative analysis showed that saponins, anthocyanin, and tannin compounds were present at both vegetative and flowering stages of Proveskia abrotanoides. Also, quantitative analysis showed the maximum amount of starch in the root and the highest concentration of soluble carbohydrates, proline, and phenolic compounds in the leaves at vegetative stage. Overall, the results showed that at the vegetative stage regardless of starch, leaves had the highest soluble carbohydrates, proline, and phenolic compounds while at the reproductive stage root contained the highest level of these compounds.