بررسي مقدماتي ساختار ژنتيكي جمعيت ميگوي سرتيز (Metapenaeus affinis) در آب هاي شمال خليج فارس به روش توالي يابي ژن 16 S rRNA

Primary study of genetic structure of Jinga shrimp (Metapenaeus affinis) population in northern Persian Gulf waters by 16S rRNA gene sequencing

با توجه به اهميت ميگوي سرتيز (Metapenaeus affinis) در چرخه صيادي خليج فارس، بررسي مقدماتي ساختار ژنتيكي جمعيت اين گونه با توالي يابي ژن ميتوكندريايي 16S rRNA انجام شد. تعداد 18 قطعه ميگو از سه منطقه بندرعباس، بوشهر و خوزستان جمع آوري و پس از استخراج DNA با روش فنل-كلروفرم، بهينه سازي واكنش PCR جهت تكثير ژن 16S rRNA انجام شد. بيش ترين مقدار فاصله ژنتيكي، بين جمعيت بندرعباس و خوزستان (0/750) و كم ترين مقدار فاصله ژنتيكي بين مناطق بوشهر و خوزستان (0/105-) به دست آمد. در سطح احتمال 0/05 تفاوت جمعيت ميگوي سرتيز منطقه بندرعباس با دو منطقه بوشهر و خوزستان معني دار بود ولي اين تفاوت جمعيتي بين بوشهر و خوزستان با يكديگر معني دار نبود كه توسط آزمون تفاوت جمعيت در داخل جمعيت ها نيز مورد تاييد قرار گرفت. ميانگين آزمون هاي بي طرفي تاجيما و شاخص Fu's FS براي 18 توالي بين مناطق به ترتيب 0/823- و 1/181 محاسبه شد كه هيچ كدام از دو شاخص از لحاظ آماري معني دار نبودند (0/05

Regarding the importance of Jinga shrimp (Metapenaeus affinis) in shrimp catch cycle in the Persian Gulf، primary study of genetic structure of this species population was considered by sequencing of mitochondrial 16S rRNA gene. A number of 18 shrimps were collected from the regions of Bandar Abbas، Bushehr and Khuzestan and after DNA extraction using phenol-chloroform method، the PCR for amplification of 16S rRNA was optimized. The maximum amount of F- statistic parameter was 0.750 calculated between samples of Bandar Abbas and Khuzestan and the minimum amount was -0.105 between the samples of Bushehr and Khuzestan. At probability level of 0.05، population differentiation was significant between Bandar Abbas and two other regions of Bushehr and Khuzestan but not significant between the regions of Bushehr and Khuzestan، which was confirmed by test of exact p values within populations. Mean values of Tajima’s D and Fu’s Fs between the regions for the 18 sequences were -0.823 and 1.181، respectively that were not statistically significant. Phylogenetic trees showed the differentiation of Bandar Abbas population from the two other regions but this hypothesis needs supplementary investigations with more samples as well as by analyzing other mitochondrial and genomic markers.