اثر عصاره آبي هسته انبه روي فركانس، قدرت و فعاليت انقباضي عضله صاف رحم بكر جداشده موش صحرايي

Effect of Aqueous Extract of Mangifera Indica Seed on the Basic Contractility, Frequency and Strength of Isolated Virgin Rat Uterine Smooth Muscle

ﻣﻘﺪﻣﻪ: ﮐﺎﻫﺶ ﻗﺪرت اﻧﻘﺒﺎﺿﯽ رﺣﻢ ﻧﻘﺶ ﻣﻬﻤﯽ در ﺗﺄﺧﯿﺮ زاﯾﻤﺎن زودرس دارد. ﻟﺬا اﯾﻦ ﭘﮋوﻫﺶ ﺑﺎ ﻫﺪف اﺛﺮ ﻋﺼﺎره آﺑـﯽ ﻫﺴـته اﻧﺒﻪ روي ﻓﺮﮐﺎﻧﺲ، ﻗﺪرت و ﻓﻌﺎﻟﯿﺖ اﻧﻘﺒﺎﺿﯽ ﭘﺎﯾﻪ رﺣﻢ ﻣﻮش ﺻﺤﺮاﯾﯽ اﻧﺠﺎم ﺷﺪ. مواد و روش‌ ها: در يك تحقيق تجربي، 12 قطعه مياني رحم موش‌ هاي سالم بكر ( Sprague Dawley- 200 تا 250 گرم)، در حمام بافت حاوي محلول تايرود كربوژنه و كشش اوليه 1 گرم قرار گرفت و انقباضات آن به روش ايزومتريك ثبت شد. اثر تجمعي عصاره آبي هسته انبه؛ ( 0/0025 تا 3000 ميكروگرم در ميلي‌ ليتر: گروه آزمايش) و حامل عصاره (گروه كنترل) بر فعاليت پايه عضله صاف رحم بكر، مورد بررسي قرارگرفت. براي مقايسه داده‌ ها از روش‌هاي آماري Repeated measure و ANOVA دو طرفه (0/05>P) اﺳﺘﻔﺎده ﮔﺮدﯾﺪ. ياﻓﺘﻪ ﻫﺎ: ﻋﺼﺎره آﺑﯽ ﻫﺴﺘﻪ اﻧﺒﻪ ﺑﻪ ﺻﻮرت ﺗﺠﻤﻌﯽ و واﺑﺴﺘﻪ ﺑﻪ دوز؛ (0/0025 ﺗﺎ 30 ﻣﯿﮑﺮوﮔﺮم در ﻣﯿﻠﯽ ﻟﯿﺘﺮ) فرﮐﺎﻧﺲ، ﻗﺪرت ﻓﻌﺎﻟﯿﺖ اﻧﻘﺒﺎﺿﯽ ﻋﻀﻠﻪ رﺣﻢ را ﺑﻪ ﺗﺮﺗﯿﺐ از 100% ﺑﻪ 68%، 65 %، 67% ﮐﺎﻫﺶ داد (p<0/05) ولي در ﻏﻠﻈﺖ ﻫﺎي 300 ﺗـﺎ 3000 ﻣﯿﮑﺮوﮔﺮم در ﻣﯿﻠﯽ ﻟﯿﺘﺮ، اﺛﺮ ﻣﻬﺎري ﻋﺼﺎره ﺑﺮ ﻓﺮﮐﺎﻧﺲ، ﻗﺪرت و ﻓﻌﺎﻟﯿﺖ اﻧﻘﺒﺎﺿﯽ را ﮐﺎﻫﺶ و ﺑﻪ ﺣﺎﻟﺖ ﭘﺎﯾﻪ ﺑﺮﮔﺮداﻧﺪ. ﻧﺘﯿﺠﻪ ﮔﯿﺮي: ﻋﺼﺎره آﺑﯽ ﻫﺴﺘﻪ اﻧﺒﻪ در ﻏﻠﻈﺖ ﻫﺎي 0/0025 ﺗﺎ 30 ﻣﯿﮑﺮوﮔﺮم در ﻣﯿﻠﯽ ﻟﯿﺘﺮ داراي اﺛـﺮ ﮐﺎﻫﺸـﯽ روي ﻓﺮﮐـﺎﻧﺲ، ﻗﺪرت و ﻓﻌﺎﻟﯿﺖ اﻧﻘﺒﺎﺿﯽ رﺣﻢ ﺑﻮده و ﻣﯽ ﺗﻮاﻧﺪ ﺑﻪ ﻋﻨﻮان ﯾﮏ داروي ﺑﺎﻟﻘﻮه در درﻣﺎن زاﯾﻤﺎن زودرس ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﯿﺮد.

Introduction: Uterine relaxations has an important role in reducing the preterm parturition. In the present study the cumulative effect of the aqueous extract of mangifera indica seed on the frequency, strength and basic smooth uterine muscle contractility of virgin rats were studied. Materials & Methods: This experimental study was conducted on 12 strips taken from the middle part of the uterine of rats (Sprague Dawley; 200-250 g), perfused in an organ bath system containing the carbogen-Tyrode solution under the continuous 1g tension. Then the contractions were recorded isometrically. During the experiments the cumulative effect of the aqueous extract of of mangifera indica seed (0.0025- 3000 μg/ml) on the frequency, strength and basal activity of rat uterine muscle was studied. The data were analyzed using the repeated measure and two way ANOVA tests at the significance level of p<0.05. Results: The cumulative effect of different concentrations of the aqueous extract of mangifera indica seed (0.0025-30μg/ml) significantly decreased the frequency, strength and contractile response of smooth uterine muscle in a dose-dependent manner from 100% to 68%, 65%, 67% (p<0.05, N=6, Repeated measure , two way ANOVA) respectively. But this effect decreases in concentrations higher than 300 μg/ml. Conclusion: Because of the relaxation effect of aqueous extract of mangifera indica seed in concentrations of 30 μg/ml on uterus, it could have the potentials for research on the treatment of preterm-parturition.