تاثير عصاره هيدرو الكلي پوست انار و ويتامين e بر ميزان نيتريك اكسيد و فعاليت آنزيم هاي پاراكسوناز و ميلوپراكسيداز در رت هاي تحت تنش اكسيداتيو ورزش درمانده ساز

Effects of Pomegranate peel hydroAlcoholic extract and vitamin E supplementation on Paraoxonase, myeloperoxidase Activities and nitric oxide levels following an exhaustive exercise in rats

مطالعات نشان داده اند كه توليد راديكال هاي آزاد در اثر تمرينات سنگين ورزشي بخصوص گونه اكسيژن (ros) باعث آسيب به بافت ها شده كه مي توان با مصرف به موقع مواد آنتي اكسيداني از اين امر جلوگيري كرد. هدف مطالعه حاضر بررسي اثرات آنتي اكسيداني عصاره هيدروالكلي پوست انار بر كاهش اثرات تنش اكسيداتيو ناشي از يك جلسه ورزش درمانده ساز بود.مواد و روش ها: در اين مطالعه تعداد 32 سر موش صحرايي نر نژاد ويستار به طور تصادفي به چهار گروه هشت تايي تقسيم شدند: 1 con (گروه كنترل). 2 pphe200 mg/kg 200 عصاره هيدروالكلي پوست انار، 3 pphe250 mg/kg 250 عصاره هيدروالكلي پوست انار و 4 vit.e mg/kg 5 ويتامين e حيوانات به مدت 8 هفته به طور منظم روزانه يك ساعت ورزش داده شدند و در آخرين جلسه تمرين تحت استرس ورزش درمانده ساز قرار گرفتند. بلافاصله پس از آخرين جلسه تمرين نمونه خون از وريد باب جهت تعيين فعاليت آنزيم هاي پاراكسوناز1، ميلوپراكسيداز و سطح نيتريك اكسيد در نمونه سرم تهيه شد.يافته ها: فعاليت آنزيم پاراكسوناز1 سرم گروه هاي Paraoxonase-1 (PON-1) activities serum were significantly increases in PPHE200 (23.03±1.47)، PPHE250 (23.59±1.98) and vit E (25.38±2.65) than in the control (18.57±1.380) (p<0.05).In PPHE200 (32.76±9.97) ،PPHE250 (31.45±6.05) and vit E (24.94±4.65) treated animals was determined in serum where myeloperoxidase activities reduced significantly compared with control (40.70±6.14) (p<0.05). Levels of Nitric oxide levels were significantly lower in PPHE 200 (46.59±2.48)، PPHE250 (40.27±2.62) and vit E (36.25±3.82) treated than in control (47.18±5.36) (p<0.05).بحث و نتيجه گيري: نتايج تحقيق نشان داد مصرف عصاره پوست انار موجب بهبود وضعيت سيستم آنتي اكسيداني، ضد التهابي و كاهش اثرات تنش اكسيداتيو ناشي از فعاليت شديد بدني مي گردد.

Background: free radicals produced as a result of heavy training exercise especially oxygen species (ROS) damage to body tissues Which can be prevent from this by consuming antioxidant substances timely. The aim of this study was to evaluate effect of Pomegranate (Punica Granatum) peel hydro alcoholic on reduced of oxidative stress induced by an exhaustive exercise. Materials and Methods: Thirty two weight-matched male Wistar rats were evenly divided into: 1) control: received saline (0.2 ml saline/ rat) by oral administration via epigastric tube. 2) Received oral administration of 200 mg/kg pomegranate peel hydro alcoholic extract (PPHE200). 3) Received oral administration of 250 mg/kg Pomegranate peel hydro alcoholic extract (PPHE250). 4) Received oral administration of vitamin E (vit E) 5 mg/kg. Animals were submitted to swimming exhaustive exercise stress for an 8-week. At the end of the experiment، blood samples were collected for serum. Serum samples were analyzed for paraoxonase-1(PON-1) and myeloperoxidase (MPO) activities and nitric oxide levels. Results: Paraoxonase-1 (PON-1) activities serum were significantly increases in PPHE200 (23.03±1.47)، PPHE250 (23.59±1.98) and vit E (25.38±2.65) than in the control (18.57±1.380) (p<0.05).In PPHE200 (32.76±9.97) ،PPHE250 (31.45±6.05) and vit E (24.94±4.65) treated animals was determined in serum where myeloperoxidase activities reduced significantly compared with control (40.70±6.14) (p<0.05). Levels of Nitric oxide levels were significantly lower in PPHE 200 (46.59±2.48)، PPHE250 (40.27±2.62) and vit E (36.25±3.82) treated than in control (47.18±5.36) (p<0.05). Conclusion: Results indicated that Pomegranate peel hydro alcoholic extract supplementations can strength antioxidant defense system and anti-inflammatory induced by exhaustive exercise.