فيلوژني گونه هاي Phytophthora و Phytopythium در شاليزارهاي برنج در استان فارس

Phylogeny of Phytophthora and Phytopythium species associated with rice in Fars province (Iran)

به منظور بررسي گونه هاي اُاُميست شاليزارهاي برنج استان فارس طي سال هاي 94-1392 از خاك، آب آبياري، ريشه و طوقه گياهان بيمار نمونه برداري انجام گرفت. براساس مطالعات ريخت شناختي، ريخت سنجي، فيزيولوژيكي جدايه ها و همچنين بررسي هاي فيلوژنتيكي فاصله ترانويسي شده داخلي (آي تي اِس) دي اِن اِي ريبوزومي با روش پيوست همسايه ها، دو گونه Phytophthora از تبارهاي 6 و 8 درخت آي تي اِس شامل P. inundata و P. pseudocryptogea و سه گونه Phytopythium در يك تبار مجزا شامل Phpy. litorale، Phpy. ostracodes و Phpy. sterile شناسايي شد. تفكيك دو گونه Phpy. litorale و Phpy. sterile از طريق مطالعات ريخت شناختي به همراه واكاوي هاي فيلوژنتيكي صورت گرفت. گونه Phpy. sterile براي فلور اُاُميستي ايران جديد است. همچنين، تمامي گونه ها براي نخستين بار در دنيا از فراريشه برنج گزارش مي شوند.

In order to investigate the Oomyceteous species of the rice paddy fields of Fars province (Iran), during 2013–15, infected roots and crowns together with soil around seedlings and irrigation water were sampled. Based on the morphological, morphometric and physiological studies along with phylogenetic analyses of internal transcribed spacer sequences based on neighbor joining method, two Phytophthora species from Clades 6 and 8, including P. inundata, and P. pseudocryptogea and three Phytopythium species from a distinct clade, including Phpy. litorale, Phpy. ostracodes, and Phpy. sterile were identified. Phytopythium litorale, and Phpy. sterile were distinguished through morphological studies along with phylogenetic analysis. Phytopythium sterile was also new to the Oomyceteous mycobiota of Iran. All species were reported for the first time in the world from rice rhizosphere.