تأثير تنش شوري و روش هاي مختلف پرايمينگ بذر بر سبز شدن و خصوصيات گياهچه كينوا (Chenopodium quinoa Willd)

Effect of salt stress and seed priming methods on emergence and seedling characteristics of quinoa (Chenopodium quinoa Willd.)

كينوا (Chenopodium quinoa) گياهي دو لپه‌اي، آلوتتراپلوئيد، از خانواده Amaranthace، سه كربنه و هالوفيت اختياري بوده كه جزو شبه غلات دسته‌بندي مي­ شود. به‌منظور بررسي اثر تنش شوري بر سبز و استقرار اوليه كينوا آزمايشي بر مبناي طرح كاملاً تصادفي با 5 سطح شوري (dS/m صفر، 4، 8، 12 و 16) و چهار تكرار در خاك و كوكوپيت بر روي ژنوتيپ NSRCNQ1 انجام شد. سبز روزانه شمارش و درنهايت وزن خشك و ارتفاع بوته اندازه ­گيري شد. معادلات دوتكه‌اي و تابع كاهشي تغييريافته بر داده ­هاي درصد سبز برازش شد. به‌منظور بهبود سبز در شرايط شور آزمايشي با سه تيمار پرايمينگ [آب، جيبرليك اسيد (ppm 10) و اسيد آسكوربيك (ppm 3)] و چهار سطح شوري (dS/m صفر، 4، 8 و 12) در سه تكرار به‌صورت فاكتوريل بر مبناي طرح كاملاً تصادفي انجام شد. نتايج نشان داد كه واكنش كينوا به شوري در مرحله سبز به بستر كاشت بستگي دارد. پنجاه‌درصد كاهش سبز در خاك و كوكوپيت به ترتيب در شوري dS/m 6.35 و 15.04 مشاهده شد و آستانه كاهش در كوكوپيت و خاك به ترتيب dS/m 9.52 و 0.03 بود. سرعت و درصد سبز بذور تحت تنش شوري در كوكوپيت تا شوري dS/m 12 اختلافي با شاهد نداشت و در شوري بالاي dS/m 4 سبز با تأخير آغازشده و ديرتر به حداكثر رسيد. ارتفاع گياهچه در خاك و كوكوپيت در شوري dS/m12 به ترتيب 91 و 30 درصد كاهش يافت. به‌طوركلي پرايمينگ موجب بهبود سبز كينوا در شرايط خاك شور نشد گرچه هيدرو پرايم تا حدي موجب بهبود سبز شد (8 درصد). بذور اين گياه قادر است بعد از رفع تنش شوري و دريافت آب با شوري مناسب شروع به سبز نمايد. كينوا بعد از مرحله گياهچه ­اي به تنش شوري تحمل بالايي دارد.

Abstract ]Introduction The main limiting factor for food security in Iran and world is water qualitative and quantitative. Agriculture sector is the main consumer of water and more vulnerable section from water crisis. In order to improve food security calorie requirement of growing population should be provided from nonsaline water resources. Quinoa (Chenopodium quinoa) is diploeid, allotetraploied, C3 and facultative halophyte from Amaranthace family and categorized in pseudo cereal group. This plant has cultivated 5000 years in Ands, and the highest cultivated area is in Bolivia near salt flats. This plant can grow successfully in different countries such as, Europe countries, USA, Canada, Africa, Morocco, Pakistan and India. Protein content is between 13.81-21.9% and flour is gluten free and provides all essential amino acid of human. Because of high potential of quinoa for providing human calories and high salt and drought tolerance it could be considered for saline and marginal area. Quinoa was selected for cultivation with saline water because of salt tolerance and economic production with saline water which are not use for conventional crop. The main problem of quinoa is emergence and stand establishment with saline water. The aim of this study is evaluation of salinity stress on plant emergence and priming effect of emergence improvement under saline condition. Materials and methods In order to evaluate salinity stress on emergence and establishment of quinoa an experiment was conducted based on CRD design with 5 levels of salinity (0, 4, 8, 12 and 16 dS/m) with four replication in soil and cocopit. Emergence recorded daily and finally plant height and dry weight was measured. Two piece and modified discount function model was fitted to emergence percent. In order to improve emergence under saline condition an experiment was conducted with three treatments (Gibberlic acid (10 ppm), H2O and Ascorbic acid (3 ppm)) and four levels of salinity (0, 4 and 8 dS/m) with three replications based on CRD design with factorial arrangement. Coefficent of equation was estimated with SAS software based on NLIN and REG procedure. Results and discussion Result showed that quinoa is sensitive in emergence stage is depends to substrate and 50 % of emergence reduction in soil and cocopit occurred at 6.35 and 15.04 dS/m. Threshold in soil and cocopit was 9.52 and 0.03 dS/m. Emergence rate and percentage of seeds under saline condition in cocpit did not have significant differences up to 12 dS/m and salinity above 4 dS/m delay emergence. Seedling emergence index under nonsaline condition was 0.96 but at 4, 8 and 12 dS/m in soil had 24, 48 and 84 % reduction. Seedling height in soil and cocopit at 12 dS/m reduced 91 and 30 %, respectively. Priming could not improve quinoa emergence although hydroprime improve emergence to some extent (8%). Quinoa is sensitive to salinity at seedling stage but after this stage could produce 2-3 t ha-1 seed yield with 15 dS/m saline water in Yazd. Since, in saline area farmers don’t have fresh water and quinoa is sensitive to saline water during emergence and early establishment. It would be better use transplanting method or not coverage of seed with soil.