بررسي اثر سميت سلولي عصاره اتانولي جوانه برگ و گل گياه سياه وليك Crataegus melanocarpa بر روي رده سلول‌هاي سرطاني پستان (MCF-7) و دهانه رحم (HeLa)

The cytotoxic effects of ethanolic extract of leaf and flower buds of Crataegus melanocarpa on human breast (MCF-7) and cervical (HeLa) cancer cell line

زمينه و هدف: در چند دهه اخير دانشمندان بر استفاده از تركيبات طبيعي براي درمان سرطان، از جمله عصاره خام گياهي يا برخي از متابوليت‌هاي ثانويه گياهي متمركز شده اند. هدف از اين پژوهش بررسي فعاليت سميت سلولي عصاره اتانولي خام تهيه شده از جوانه برگ و گل گياه سياه وليك Crataegus melanocarpa بر لاين‌هاي سلولي سرطاني MCF-7 و HeLa. مواد و روشها: در اين پژوهش اثر غلظت‌هاي مختلف عصاره بر لاين هاي سلولي MCF-7 و HeLa، با استفاده از روش رنگ‌آميزي و آزمون MTT تعيين شد. چگالي نوري محلول رنگي در طول موج 570 نانومتر توسط دستگاه الايزا ريدر، پس از 24 و 48 و 72 ساعت انكوباسيون، تعيين شد. يافته ها: نتايج نشان دادند كه پس از 48 ساعت انكوباسيون، عصاره اتانولي در غلظت‌هاي 5 و mg/ml 10 تكثير سلول‌هاي سرطاني MCF-7 را با تفاوت معني‌دار با سلول‌هاي كنترل، كاهش داد .

Background & Objectives In the last few decades, researches hane been focused on the use of natural products for cancer therapy, such as crude plant extracts or some of different plant secondary metabolits. The objective of this study was to examine the invitro cytotoxic activities of crude ethanolic extract of leaf and flower buds of Crataegus melanocarpa, on human MCF-7 and HeLa cell lines, with different concentrations, by Dye exclusion and Micro culture tetrazolium test (MTT) assay. Materials & Methods The optical density (OD) colored solution was quantified at 570 nm wavelengths by an ELISA Reader, after 24, 48 and 72 h incubation. The results showed that ethanolic extracts. Results After 48h incubation, at in 5 and 10 mg/ml suppressed the proliferation of cancerous MCF-7 cells by significant difference with control group (p<0.05). Also, after 72h incubation, concentrations of 0.156 mg/ml and upper doses had significant different with control group; and maximum inhibitory effects were showed at 10 mg/ml (86.14%). Results showed that the C. melanocarpa extracts suppressed dose dependently the proliferation of MCF-7 cells. Also, results showed that cytotoxic effects of C. melanocarpa extracts on Hela cell line is very low, and in 10 mg/ml concentrating, after 72h incubation, suppressed the proliferation of cancerous MCF-7 cells by significant diffrence with control group (p<0.05). Maximum inhibitory effects were found at 10 mg/ml (23.14%). Conclusion Finally, this study emphasizes C. melanocarpa to be employed in new cancer therapeutic drugs and provide the basis for future research on the various species of this genus.