بررسي تاثير ويتامين D3 و عصاره زنجبيل بر علايم باليني و شدت التهاب در بيماري آنسفالوميليت خود ايمن تجربي

Evaluation of the Effect of Vitamin D3 and Ginger Extract on the Clinical Symptoms and the Severity of Inflammation in EAE

بيماري آنسفالوميليت خودايمن تجربي(EAE) با ارتشاح لكوسيتي سيستم عصبي مركزي همراه مي باشد. مطالعات نشانگر اثرات ضد التهابي ويتامين D3 و زنجبيل مي باشد. هدف اين مطالعه بررسي اثر ويتامينD3 و عصاره زنجبيل بر روي علايم باليني EAE و ميزان ارتشاح لكوسيتي است. مواد و روش ها مطالعه حاضر از نوع تجربي مي باشد. القاء بيماري EAEبا ايمونيزاسيون موش هاي C57BL/6 با تزريق پپتيد 55-35 MOG مخلوط شده با ادجوانت كامل فروند صورت گرفت. گروه هاي مبتلا به EAE شامل دريافت كننده هاي ويتامين D3، عصاره زنجبيل، روغن زيتون، بافر فسفات سالين و همچنين PBS به عنوان كنترل تقسيم گرديدند. در روز 31 موش هاي مورد مطالعه كشته شدند و علايم باليني، شدت التهاب بيماري و تغييرات وزن نيز تا 30 روز مورد بررسي قرار گرفت. داده هاي حاصل توسط آزمون آماري t-test مورد بررسي قرار گرفت. نتيجه گيري نتايج اين مطالعه نشان مي دهد كه ويتامين D3 و عصاره زنجبيل مي تواند در جلوگيري از پيشرفت بيماري و نيز كاهش ارتشاح لكوسيتي در CNS موثر باشند.

The anti-inflammatory effects of the vitamin D and ginger have been demonstrated in some studies. The aim of this study was to evaluate the effects of vitamin D and ginger extract on the clinical symptoms and leukocyte infiltration of experimental autoimmune encephalomyelitis (EAE). Materials an‎d Methods For the induction of EAE, the (C57BL/6) were immunized subcutaneously MOG peptide emulsified in complete Freund's adjuvant. The mice divided into EAE group includimg: vitamin D-treated, ginger extract-treated, olive oil and phosphate-buffered saline, and also one group which was considered as normal group and received only PBS(phosphat- Buffered Saline). The mice were sacrificed at day 31 after immunization and the leukocytes infiltration was evaluated in the CNS (central nervous system). The EAE scores and the body weight were evaluated till day 30. Data were analyzed using t-test. Results Two control groups showed the clinical symptoms at day 10, whereas vitamin D and ginger-treated groups exhibited the symptoms at days 13 and 15, respectively. The maximum mean score of disease was (1.87±1.61), (1.92±1.79), (1.05±1.01), and (0.73±0.81) for olive oil-, PBS-, vitamin D3-, and ginger-treated groups, respectively. Vitamin D3 and ginger extract increased the body weight significantly)P =0.05) as compared to control groups. The leukocytes infiltration was also significantly lower in treated groups as compared to control group. Conclusion These observations represent that the vitamin D3 and ginger extract have significant capability to attenuate the EAE severity.