توليد داربست سلولز باكتريايي حاوي عصاره گياه و بررسي اثرات التيام بخشي آن بر زخمهاي جلدي موش صحرايي

Production of bacterial cellulose scaffold contains herb extract and assessment of its healing effects in rat cutaneous wounds

مقدمه: سلولز پليمري طبيعي با قابليت ترميم زخم مي باشد كه مي توان خاصيت ترميم زخم آن را با تركيب نمودن با برخي از عصاره هاي گياهي بهبود بخشيد. هدف از اين مطالعه بررسي توليد سلولز باكتريال حاوي عصاره گياه زنجبيل و ارزيابي اثرات التيام بخشي آن بر زخمهاي جلدي موش صحرايي مي باشد. روش بررسي: در اين مطالعه تجربي، ابتدا Ghiconacetobacter aylinuts در محيط كشت هيسترين اسكرام در شرايط ايستا كشت داده شد و هم چنين جوشانده گياه Zingiber officinale تهيه شد. سپس تست (Microculture Tetrazolium Test ( NTT براي بررسي ميزان سميت سلولي جوشانده گياه Zingiber officinale انجام شد و در نهايت مراحل روند التيام بخشي بر روي زخم هاي جلدي موش صحرايي در روزهاي مختلف درمان مورد بررسي قرار گرفت. نتايج: نتايج حاصل از اين تحقيق نشان داد كه لايه سلولز توليدي محكم، بدون شكستگي و با 7/005 =pH بوده است كه داراي قدرت ترميم زخم 80٪ مي باشد. عصاره گياه Zingiber officinale در تست MTT داراي سميت براي سلول ها بود كه اين سميت براي سلول هاي سرطاني و غيرسرطاني يكسان بوده است. جهت ترميم زخم از غلظت غيرسمي عصاره گياه استفاده شد و هم چنين نتايج ترميم زخم نشان داد كه سلولز و عصاره Zingiber officinale مي توانند به عنوان دارويي مناسب براي ترميم زخمها استفاده شوند. نتيجه گيري: گونه Gluconacetobacter aylinuts در التيام و بهبود زخم موثر بوده و هم چنين گياه زنجبيل در درمان زخم هاي باز اثر معجزه آسايي دارد.

Introdution: Natural cellulose is a normal wound healing remedy that can improve the wound healing properties by combining with some herbal extracts. The aim of this study was to evaluate the production of bacterial cellulose containing Zingiber extract and to evaluate its healing effects in rat cutaneous wounds. Methods: In this experimental study, Gluconacetobacter xylinus was first cultured in a hysterine-scrum culture medium under static conditions and aqueous extract of Zingiber officinale plant was prepared. Then, Microculture Tetrazolium Test (MTT) was performed to evaluate the cytotoxicity of the aqueous extract of Zingiber officinale, and finally the healing process was investigated on rat cutaneous ulcers in different days of treatment. Results: The results of this study have shown that the cellulose layer was manufactured, tight, non-fracture and with pH = 7/005, which had the proper repair wound of 80%. The extract of the Zingiber officinale plant in the MTT test had toxicity to the cells, which has the same toxicity for cancerous and non-cancerous cells. For wound healing, the non-toxic concentration of the extract was used, and the results of the wound healing showed that cellulose and Zingiber officinale extract can be used as a suitable drug for wound healing. Conclusion: Gluconacetobacter xylinus is effective in healing and wound, also the Zingiber officinale plant has a miraculous effect on open wounds.