ﺳﻨﺘﺰ ﻧﻘﺎط ﮐﻮاﻧﺘﻮﻣﯽ ﮔﺮاﻓﻦ ﺑﺮاي ﺗﺸﺨﯿﺺ اﺳﺘﻮن ﺑﺎزدم ﺑﯿﻤﺎران ﻣﺒﺘﻼ ﺑﻪ دﯾﺎﺑﺖ ﻣﯿﻠﺘﻮس

Synthesis of Solution Graphene Quantum Dots for Diagnostic Diabetes Mellitus by Exhaled Breath

ﻧﻘﺎط ﮐﻮاﻧﺘﻮﻣﯽ ﮔﺮاﻓﻦ (GQDs) ﺑﻪ دﻟﯿﻞ وﯾﮋﮔﯽﻫﺎي اﭘﺘﯿﮑﯽ ﺑﺎرز، ﻣﯽﺗﻮاﻧﻨﺪ ﺑﺮاي ﺗﺸﺨﯿﺺ ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﮔﯿﺮﻧﺪ. ﻧﺎﻧﻮ ﻧﻘﺎﻃﯽ ﮐﻪ از روش ﭘﯿﺮوﻟﯿﺰ ﺳﻨﺘﺰ ﻣﯽﺷﻮد، ﺑﻪ دﻟﯿﻞ وﯾﮋﮔﯽﻫﺎي ﺳﺎﺧﺘﺎري ﺑﺮاي ﺗﺸﺨﯿﺺ اﺳﺘﻮن ﺑﺎزدم ﺑﯿﻤﺎران ﻣﺒﺘﻼ ﺑﻪ دﯾﺎﺑﺖ ﻣﯿﻠﺘﻮس ﻣﻨﺎﺳﺐ ﻣﯽ ﺑﺎﺷﻨﺪ. در اﯾﻦ ﭘﮋوﻫﺶ، ﻧﻘﺎط ﮐﻮاﻧﺘﻮﻣﯽ ﺳﻨﺘﺰ ﺷﺪه ﮔﺮاﻓﻦ اﺑﺘﺪا ﺑﺎ اﺳﺘﻔﺎده از ﻣﺸﺨﺼﻪ ﯾﺎﺑﯽ FTIR ،TEM و -UV Vis از ﻧﻈﺮ ﺳﺎﺧﺘﺎري ﺑﺮرﺳﯽ ﻣﯽﺷﻮﻧﺪ. ﻃﯿﻒ ﻓﻮﺗﻮﻟﻮﻣﯿﻨﺴﺎﻧﺲ ﻣﺤﻠﻮل اﯾﻦ ﻧﺎﻧﻮ ذرات ﻃﻮل ﻣﻮج ﺑﺮاﻧﮕﯿﺨﺘﮕﯽ در 360 ﻧﺎﻧﻮﻣﺘﺮ و ﺑﯿﺸﯿﻨﻪ ﺷﺪت ﻃﯽ77/4 % در ﻃﻮل ﻣﻮج 481 ﻧﺎﻧﻮﻣﺘﺮ را ﻧﺸﺎن ﻣﯽ دﻫﻨﺪ. ﺗﺼﺎوﯾﺮ TEM ﻧﺸﺎن ﻣﯽدﻫﺪ GQDs ﺳﻨﺘﺰ ﺷﺪه ﺑﻪ ﻃﻮر ﻣﯿﺎﻧﮕﯿﻦ اﻧﺪازه اﯾﯿﺰﯾﺮ 10 ﻧﺎﻧﻮﻣﺘﺮ دارﻧﺪ. ﻃﯿﻒﻫﺎي FTIR و UV-Vis از اﯾﻦ ﺣﮑﺎﯾﺖ دارد ﮐﻪ ﭘﺎﺳﺦ اﯾﻦ ﻧﺎﻧﻮذرات ﺑﻪ ﺑﺎزدم اﻓﺮاد، ﺧﺎﻣﻮﺷﯽ ﯾﺎ ﮐﺎﻫﺶ ﺷﺪّت اﭘﺘﯿﮑﯽ را ﻧﺸﺎن ﻣﯽدﻫﺪ، ﮐﻪ ﻋﻠﺖّ آن وﺟﻮد ﺳﺎﺧﺘﺎر ﮐﺘﻮﻧﯽ در ﺑﺎزدم اﺳﺖ. اﺳﺘﻮن ﺑﻪ ﻋﻨﻮان ﺳﺎدهﺗﺮﯾﻦ ﮐﺘﻮن در ﺑﺮﺧﻮرد ﺑﺎ ﻣﻮﻟﮑﻮلﻫﺎي دﯾﮕﺮ ﺑﻪ وﯾﮋه اﮐﺴﯿﮋنﻫﺎي ﮔﺮوهﻫﺎي ﻋﺎﻣﻠﯽ ﻟﺒﻪ GQDs- ﺑﻪ ﺣﺎﻟﺖ ﭘﺎﯾﻪ ﺑﺮﮔﺸﺘﻪ و ﻧﻤﻮدارﻫﺎي ﻃﯿﻒ ﺳﻨﺠﯽ، ﺧﺎﻣﻮﺷﯽ اﭘﺘﯿﮑﯽ ﻣﺤﻠﻮل GQDs را ﻧﺸﺎن ﻣﯽدﻫﺪ.

Graphene quantum dots (GQD) can be used for diagnosis because of its high Optical properties. Quantum dots which were synthesized by pyrolysis method, used for detection of acetone as in diabetes mellitus breath. In this project, the Graphene quantum dots were characterize by TEM, FTIR and UV-vis. The photolominesence spectrum of the nanoparticle solution shows the excitation in 360 nm and the maximum of spectrum intensity of %77.4 at 481 nm wavelength. The TEM graphs reveal that the sizes of GQDs are in average about 4.48 nm. The UV-vis and FTIR spectrums show that the responses to exhaled breath cause to quenching or decrease optical intensity due to acetone. Acetone molecules as simplest ketoneare deexcited to initial state because of collision with other molecules, especially with oxygen-containing functional groups and the spectrum plots show the optical quenching of GQDs solution.