بررسي اثر لسيتين سويا بر برخي شاخص هاي ايمني و بيوشيمي خون پيش مولدين ماهي آزاد درياي خزر (Salmo trutta caspius )

The effect of soybean lecithin on immunity and some biochemical indices of Salmo trutta caspius

مطالعه حاضر با هدف ارزيابي اثر استفاده لسيتين سويا بر برخي از پارامترهاي فيزيولوژي نظير ايمني، هماتولوژيك، بيوشيميايي سرم خون و آنزيم هاي آنتي اكسيداني پيش مولدين ماهي آزاد درياي خزر انجام پذيرفت. براي اين منظور تعداد 90 قطعه ماهي (10±350گرم) بصورت تصادفي در سه تيمار (هر كدام با 3 تكرار) تقسيم شدند. ماهيان گروه اول (شاهد) فقط با غذاي پايه، گروه دوم غذاي پايه به همراه 12% روغن سويا، گروه سوم غذاي پايه بعلاوه 6% روغن سويا و 6% لسيتين به مدت 90 روز تغذيه شدند. نتايج فراسنجه هاي ايمني، فعاليت آنزيم هاي گلوتاتيون S ترانسفراز، سوپراكسيد ديسموتاز به همراه شاخص هاي بيوشيميايي سرم خون به غير از VLDL و تري گليسيريد بيانگر برتري آماري تيمار 2 و 3 نسبت به تيمار شاهد بود (0/05<). VLDLنيز در تيمار 1 بيشتر از تيمارهاي 2 و 3 (تغذيه با روغن سويا و لسيتين) بود (0/05

The present study was to evaluate the effect of soybean lecithin on some immunological parameters (Lysozyme, ACH50 and Total antibody), hematological and serological parameters, and liver antioxidant enzymes activity of Salmo trutta caspius. For this purpose, 90 fish (350g ± 10) were randomly divided into three treatments with three respective replicates. The first group (control) was fed only with commercial feed, while the second group was fed with diet supplemented with 12% soybean oil, and the third one received feed supplemented with 6% soybean oil and 6% soybean lecithin for 90 days. In treatments 2 and 3, immunological, glutathione S transferase and superoxide dismutase activities and some biochemical parameters (LDL, HDL and Cholesterol), except VLDL and triglyceride, were significantly higher than in the control treatment (P<0.05). VLDL of treatment 1 was significantly higher than that of treatments 2 and 3 (those fish received soybean oil and/or lecithin). Hematological indices of treatment 2 including RBC, Hb and Hct, and Hct and WBC of treatment 3 were significantly different from treatment 1 (P<0.05). In conclusion, addition of 6% soybean lecithin as a supplementary feed to the diet of Caspian Sea salmon (Salmo trutt caspius) is suggested due to its ameliorative effects on physiological indices.