اثر فروليك اسيد بر اختلال عملكرد شناختي و نقص ساختاري مغز در موش‌هاي مبتلا به مولتيپل اسكلروزيس ناشي از كوپريزون

The Effect of Ferulic Acid on Cognitive Disorder an‎d Brain Structural Deficit in Mice in Cuprizone Induced Animal Model of Multiple Sclerosis

زمينه و هدف: فروليك اسيد تركيبي فنولي است كه در غلات مختلف يافت مي‌گردد و مطالعات انجام‌شده نشان مي‌دهند كه اين تركيب داراي اثرات آنتي‌اكسيداني، ضد‌آپوپتوزي و تمايز‌دهندگي در سلول‌هاي عصبي است. هدف از اين مطالعه، بررسي اثر فروليك اسيد بر پيشگيري از اختلال حافظه ناشي از كوپريزون در مدل مولتيپل اسكلروزيس ناشي از كوپريزون در موش‌هاي نر نژاد C57B6/J بود. روش بررسي: دميليناسيون به‌وسيله افزودن كوپريزون به ميزان 0/2% به غذاي معمول حيوانات به مدت 6 هفته القا شد. فروليك اسيد با 10 ميلي‌گرم به ازاي وزن بدن يا 100 ميلي‌گرم به ازاي وزن بدن روزانه به حيوانات خورانده شد. در پايان هفته ششم آزمون رفتاري ماز Y و شاتل باكس جهت ارزيابي اختلال رفتاري و حافظه ناشي از دميليناسيون نوروني و بررسي‌هاي بافت‌شناسي با رنگ‌آميزي‌هاي لوكسال فاست بلو انجام شد. يافته‌ها: نتايج اين مطالعه نشان داد كه فروليك اسيد در دو دوز از ميزان ورود تصادفي حيوانات به بازوهاي ماز Y نسبت به گروه كنترل ‌مي‌كاهد (0/01

Background and Objective: Ferulic acid is a polyphenolic compound derived from some fruit and different cereals. Previous studies have shown that this compound has antioxidant, anti-apoptosis, and differentiation properties in the nervous system. The aim of this study was to evaluate the effect of ferulic acid on the death of oligodendrocytes and demyelination in male C57B6 / J mice in cuprizone induced animal model of multiple sclerosis. Material and methods: In this study, demyelination was induced by oral administration of rats with the 0.2% cuprizone that was added to the usual diet during 6 weeks. Animals were fed daily with ferulic acid at doses of 10or 100 mg / kg of body weight. At the end of the sixth week, the passive avoidance and Y maze test were performed. Then, animal brains were removed and fixed to histological studies using Luxol–Fast-Blue staining Results: The results of this study showed that ferulic acid reduced the random entry of animals into the arms of the Y maze in compared to the cuprizone treated group [p<0.01], but the sequential entry of animals into the Y maze were significantly increased [P<0.05]. A significant improvement in passive avoidance test was observed in the groups treated with ferulic acid at dose of 10 [p<0.05]. Histological evaluations showed the lower demyelination in Luxol-Fast-Blue in the group treated with ferulic acid. Conclusion: The results of this study showed that ferulic acid plays a neuroprotective role in cuprizone models of multiple sclerosis through reducing neuronal demyelination and oligodendrocytes death.