ﺑﺮرﺳﯽ اﺛﺮات آﻧﺘﯽ ﻫﯿﺴﺘﺎﻣﯿﻦ دﯾﻤﻦ ﻫﯿﺪرﯾﻨﺎت ﺑﺮ آﻧﺰﯾﻢ ﻫﺎي ﺗﺴﺖ ﻋﻤﻠﮑﺮد ﮐﺒﺪي و ﺑﺎﻓﺖ ﮐﺒﺪ در ﻣﻮش ﻫﺎي ﺻﺤﺮاﯾﯽ ﻧﺮ

The Study of Dimenhydrinate Antihistamine`s Effects on Liver`s Tissue and Enzymes in Male Rats

دﯾﻤﻦ ﻫﯿﺪرﯾﻨﺎت ﺑﻪ ﻋﻨﻮان ﯾﮏ داروي ﺿﺪ ﺗﻬﻮع و آﻧﺘﯽ رﻓﻼﮐﺲ ﻣﻌﺪي در ﻣﻮارد ﺗﻬﻮع ﻧﺎﺷﯽ از ﻣﺴﺎﻓﺮت ﻣﻮرد اﺳﺘﻔﺎده ﻣﯽ ﺑﺎﺷﺪ. اﯾﻦ دارو ﺑﻪ راﺣﺘﯽ از راه ﺧﻮراﮐﯽ ﺟﺬب ﻣﯽ ﮔﺮدد. ﻣﺘﺎﺑﻮﻟﯿﺴﻢ دﯾﻤﻦ ﻫﯿﺪرﯾﻨﺎت ﮐﺒﺪي اﺳﺖ و از ﮐﻠﯿﻪ ﻫﺎ ﺑﻪ ﺻﻮرت ﻣﺘﺎﺑﻮﻟﯿﺖ دﻓﻊ ﻣﯽ ﮔﺮدد. در اﯾﻦ ﺗﺤﻘﯿﻖ ﺑﻪ ﺑﺮرﺳﯽ اﺛﺮات داروي آﻧﺘﯽﻫﯿﺴﺘﺎﻣﯿﻦ دﯾﻤﻦ ﻫﯿﺪرﯾﻨﺎت ﺑﺮ ﺑﺎﻓﺖ ﮐﺒﺪ و آﻧﺰﯾﻢ ﻫﺎي ﮐﺒﺪي و ﺗﺄﺛﯿﺮات ﺑﺎﻟﻘوه ﺳﻮء ﺗﺠﻮﯾﺰ ﺑﯿﺶ از ﺣﺪ اﯾﻦ دارو ﭘﺮداﺧﺘﻪ اﯾﻢ. از 40 ﺳﺮ ﻣﻮش ﺻﺤﺮاﯾﯽ ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر در ﻣﺤﺪوده وزﻧﯽ 20 ± 250 ﮔﺮم اﺳﺘﻔﺎد ﺷﺪ. رت ﻫﺎ در ﭼﻬﺎر ﮔﺮوه ده ﺗﺎﯾﯽ ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﯾﮏ ﮔﺮوه ﺗﯿﻤﺎر ﺑﺎ دوزﻫﺎي ﻣﺨﺘﻠﻒ 500 ،250 و 750 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﻧﺴﺒﺖ ﺑﻪ وزن ﺑﺪن از دارو ﺑﻪ ﺻﻮرت ﺧﻮراﮐﯽ روزاﻧﻪ ﺑﻪ ﻣﺪت 28 روز داده ﺷﺪ. ﮐﻪ در ﭘﺎﯾﺎن دوره، ﺣﯿﻮاﻧﺎت را ﺑﺎ اﺳﺘﻔﺎده از اﺳﺘﻨﺸﺎق اﺗﺮ ﮐﺸﺘﻪ و از ﺧﻮن ﺟﻬﺖ ﺗﺴﺖﻫﺎي آﻧﺰﯾﻤﯽ و ﮐﺒﺪ ﺟﻬﺖ اﻧﺠﺎم آزﻣﺎﯾﺸﺎت ﭘﺎﺗﻮﻟﻮژي ﻧﻤﻮﻧﻪﺑﺮداري ﺑﻌﻤﻞ آﻣﺪ. ﻣﻘﺎﯾﺴه ﺳﻄﺢ آﻧﺰﯾﻢﻫﺎي ALT AST و ALP در ﮔﺮوه ﻫﺎي ﺗﯿﻤﺎر 5 ،2/5 و 7/5 در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﮐﻨﺘﺮل اﻓﺰاﯾﺶ ﻧﺸﺎن داد )0/05

In this study, we checked the distractive effects of dimenhydramine antihistamine on liver`s tissue and enzymes thereinafter excessive prescription. Dimenhydramine is a drug with anti-gastric reflux and anti-nausea effects usually used in trip for sensitive persons. This drug absorbed easily from oral usage and during 24 h excreted from kidneys and it has hepatic metabolism. We used 40 male Wistar adult rats whit 250+20 weighting that grouped into four groups. The control group was without treatment and the other fed with 2.5, 5, 7.5 mg/kg of dienhydramine doses daily within 28 d. After the examination procedure, rats became unconscious with ether, blood sampling and liver`s biopsy were done and sent to the laboratory for more study. Comparing the enzyme levels of ALT, AST, and ALP in the treatment and control groups, there was a rise in the enzyme levels as a result of liver damage caused by taking dimenhydramine antihistamine. Observing the microscopic slides of the liver tissue of the treatment group, it suggests sinusoid destruction, the loss of bile ducts, irregular placement of adjacent cells and the absence of Kupffer cells, which in turn confirms the negative effect of this drug on liver tissue. Like other medications, dienhydramine can exert adversary effects as well as positive effects. Since one of the tissues which are susceptible to this effect is liver, we must bear in mind when it comes to prescribing this medication.