تأثير عصاره هيدروالكلي برگ گياه گل ساعتي (Passiflora cearulea) بر برخي فاكتورهاي بيوشيميايي در موش صحرايي نر بالغ

The Effect of Hydroalcoholic Extract of Passiflora caerulea Leaf on Some Biochemical Factors in Adult Male Rat

ﮔﻞ ﺳﺎﻋﺘﯽ از ﺟﻤﻠﻪ ﮔﯿﺎﻫﺎن داروﯾﯽ اﺳﺖ ﮐﻪ داراي ﺗﺮﮐﯿﺒﺎﺗﯽ ﺑﺎ ﺧﻮاص آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ اﺳﺖ و در درﻣﺎن ﺑﯿﻤﺎريﻫﺎي ﺻﺮع، اﺳﻬﺎل، ﺳﻮﺧﺘﮕﯽ و ﻫﻤﻮروﯾﯿﺪ و ﺗﻨﻈﯿﻢ آﻧﺰﯾﻢﻫﺎي ﮐﺒﺪي ﻣﻔﯿﺪ ﻣﯽﺑﺎﺷﺪ. ﻫﺪف از اﻧﺠﺎم اﯾﻦ ﭘﮋوﻫﺶ ﺑﺮرﺳﯽ ﺗﺎًﺛﯿﺮ ﺗﺠﻮﯾﺰ ﺧﻮراﮐﯽ ﻋﺼﺎره ﮔﻞ ﺳﺎﻋﺘﯽ ﺑﺮ ﻏﻠﻈﺖﻫﺎي ﭘﻼﺳﻤﺎﯾﯽ اوره، ﮐﺮاﺗﯿﻨﯿﻦ، آﻟﺒﻮﻣﯿﻦ و ﭘﺮوﺗﺌﯿﻦ ﺗﺎم در ﻣﻮش ﺻﺤﺮاﯾﯽ ﻧﺮ ﺑﺎﻟﻎ ﺑﻮد. ﺗﻌﺪاد 40 ﺳﺮ ﻣﻮش ﺑﺎ وزن ﺗﻘﺮﯾﺒﯽ 195 ﮔﺮم ﺑﻪ ﭘﻨﺞ ﮔﺮوه ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﮔﺮوه ﮐﻨﺘﺮل ﻫﯿﭻ ﮔﻮﻧﻪ ﺗﯿﻤﺎر داروﯾﯽ درﯾﺎﻓﺖ ﻧﮑﺮدﻧﺪ. ﮔﺮوه ﺷﺎﻫﺪ، ﻓﻘﻂ آب ﻣﻘﻄﺮ در ﯾﺎﻓﺖ ﻧﻤﻮد. ﮔﺮوهﻫﺎي ﺗﺠﺮﺑﯽ، ﺑﻪ ﻣﺪت 21 روز ﺑﻪ ﺗﺮﺗﯿﺐ ﻣﻘﺎدﯾﺮ 300 ،150 و 600 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﻋﺼﺎره ﺑﺮگ ﮔﻞ ﺳﺎﻋﺘﯽ درﯾﺎﻓﺖ ﮐﺮدﻧﺪ. ﺑﻌﺪ از ﭘﺎﯾﺎن اﯾﻦ دوره ﺑﻪ ﻣﻨﻈﻮر اﻧﺪازهﮔﯿﺮي ﻓﺎﮐﺘﻮرﻫﺎي ﻣﻮرد ﻣﻄﺎﻟﻌﻪ )ازت اوره، ﮐﺮاﺗﯿﻨﯿﻦ، آﻟﺒﻮﻣﯿﻦ، ﭘﺮوﺗﺌﯿﻦ ﺗﺎم( ﺧﻮﻧﮕﯿﺮي از ﻗﻠﺐ اﻧﺠﺎم ﺷﺪ. ﻧﺘﺎﯾﺞ ﺑﺪﺳﺖ آﻣﺪه ﺑﺎ اﺳﺘﻔﺎده از ﻧﺮماﻓﺰار SPSS ﻣﻮرد ﺗﺠﺰﯾﻪ و ﺗﺤﻠﯿﻞ ﻗﺮار ﮔﺮﻓﺖ و ﻣﻘﺎﯾﺴﻪ ﻣﯿﺎﻧﮕﯿﻦﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮن T ﺗﺴﺖ اﻧﺠﺎم ﺷﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﻣﻘﺪار آﻟﺒﻮﻣﯿﻦ در ﮔﺮوه ﺗﺠﺮﺑﯽ درﯾﺎﻓﺖ ﮐﻨﻨﺪه 600 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﻋﺼﺎره، ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل ﮐﺎﻫﺶ ﻣﻌﻨﯽداري داﺷﺖ. ﻣﯿﺰان ﭘﺮوﺗﺌﯿﻦ ﺗﺎم، ﮐﺮاﺗﯿﻨﯿﻦ در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﮐﻨﺘﺮل ﺗﻐﯿﯿﺮات ﻣﻌﻨﯽداري ﻧﺸﺎن ﻧﺪاد. ﻫﻤﭽﻨﯿﻦ ﻋﺼﺎره آﺑﯽ- اﻟﮑﻠﯽ ﺑﺮگ ﮔﯿﺎه ﮔﻞ ﺳﺎﻋﺘﯽ ﺑﺎﻋﺚ ﮐﺎﻫﺶ ﻣﯿﺰان ازت اوره ﺧﻮن در ﮐﻠﯿﻪ ﮔﺮوهﻫﺎي ﺗﺠﺮﺑﯽ درﯾﺎﻓﺖ ﮐﻨﻨﺪه ﻋﺼﺎره ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل ﺷﺪ. ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻣﯽﺗﻮان اﯾﻨﻄﻮر ﺑﯿﺎن ﮐﺮد ﮐﻪ ﮔﯿﺎه ﮔﻞ ﺳﺎﻋﺘﯽ ﻧﻘﺶ ﺣﻤﺎﯾﺖﮐﻨﻨﺪﮔﯽ ﺑﺮاي ﮐﺒﺪ اﯾﺠﺎد ﻣﯽﮐﻨﺪ و ﻋﺪم آﺳﯿﺐ ﮐﺒﺪي در رتﻫﺎي ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎندﻫﻨﺪه اﯾﻦ ﻣﻮﺿﻮع ﺑﻮد

Passiflora caerulea is one of medicinal plants that has contained high anti-oxidant combinations and are useful in curing epilepsy, diarrhea, burn, Hemorrhoid and in regulating liver enzymes. The objective of this study was evaluation of effect of passiflora caerulea leaf hydroalcoholic extract on some biochemical factors in adult male rat. 40 male rats, about 195-200 g each were used in five groups. The control group did not receive any drug treatment, sham group found only distilled water, and experimental groups receiving 150,300,600, mg/kg leaves of passiflora caerulea Hydro-alcohol extract respectively during 21 days. After the end of this period, blood samples were taken from the heart to measure the biochemical factors (urea, creatinine, albumin, total protein). The results were analyzed using SPSS software and comparison of meanings was done using T. test. The results showed that the amount of albumin in the experimental group receiving 600 mg / kg of the extract decreased significantly compared to the control group. Total protein, cratinin and total body weight had no significant changes with control group. Hydro-alcoholic extracts of Passionate flower leaf caused reduction of blood urea nitrogen levels in all experimental groups receiving the extracts as compared to the control. According to the results of this study, it can be argued that the Passiflora caerulea plays a supporting role for the liver, and the lack of liver damage in the rats indicated this issue.