ﺑﺮرﺳﯽ اﺛﺮ ﻣﺤﺎﻓﻈﺘﯽ ﻋﺼﺎرهﻫﺎي ﻣﺮزه ﺑﺨﺘﯿﺎري و آوﯾﺸﻦ دﻧﺎﯾﯽ ﺑﺮ ﻓﯿﺒﺮوز ﮐﺒﺪي ﻧﺎﺷﯽ از ﺗﯿﻮاﺳﺘﺎﻣﯿﺪ در ﻣﻮشﻫﺎي ﺻﺤﺮاﯾﯽ ﻧﺮ

Protecting Effect Extracts of Satureja bachtiarica and Thymus daenensis Celak against Hepatic Fibrosis Induced by Thioacetamide on Rat

اﻣﺮوزه ﯾﮑﯽ از ﻣﺸﮑﻼت ﺟﻮاﻣﻊ ﺑﺸﺮي ﺑﯿﻤﺎريﻫﺎي ﮐﺒﺪي و اﺳﺘﻔﺎده از داروﻫﺎي ﺷﯿﻤﯿﺎﯾﯽ ﺑﺎ ﻋﻮارض ﺟﺎﻧﺒﯽ ﻣﯽﺑﺎﺷﺪ ﮐﻪ ﺑﺎﻋﺚ ﺗﻐﯿﯿﺮ روﯾﮑﺮد ﻣﺤﻘﻘﯿﻦ ﺑﻪ ﺳﻤﺖ ﻣﻨﺎﺑﻊ داروي ﮔﯿﺎﻫﯽ ﺟﺪﯾﺪ ﺷﺪه اﺳﺖ. ﻫﺪف از اﻧﺠﺎم اﯾﻦ ﺗﺤﻘﯿﻖ ﺑﺮرﺳﯽ اﺛﺮ ﻋﺼﺎره ﻣﺮزه ﺑﺨﺘﯿﺎري آوﯾﺸﻦ دﻧﺎﯾﯽ ﺑﺮ آﺳﯿﺐﻫﺎي ﮐﺒﺪي ﻧﺎﺷﯽ از ﺗﺰرﯾﻖ ﺗﯿﻮاﺳﺘﺎﻣﯿﺪ ﻣﯽﺑﺎﺷﺪ. ﭘﻨﺠﺎه و ﭼﻬﺎر ﺳﺮ رت ﻧﺮ را ﺑﻪ ﺻﻮرت ﺗﺼﺎدﻓﯽ اﻧﺘﺨﺎب ﮐﺮده و ﺑﻪ ﻧﻪ ﮔﺮوه ﺷﺶﺗﺎﯾﯽ ﺗﻘﺴﯿﻢ ﺑﻨﺪي ﮐﺮده ﮔﺮوه )اول ﺷﺎﻫﺪ، دوم ﮐﻨﺘﺮل ﻣﺜﺒﺖ، ﺳﻮم ﮐﻨﺘﺮل ﻣﻨﻔﯽ، ﭼﻬﺎرم، ﭘﻨﺠﻢ و ﺷﺸﻢ ﺗﺤﺖ درﻣﺎن ﺑﺎ ﻋﺼﺎره ﻣﺮزه ﺑﺨﺘﯿﺎري ﺑﺎ دوز 10 ،5 و 20 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم و ﻫﻔﺖ، ﻫﺸﺖ و ﻧﻪ ﺗﺤﺖ درﻣﺎن ﺑﺎ ﻋﺼﺎره آوﯾﺸﻦ دﻧﺎﯾﯽ ﺑﺎ دوز 10 ،5و 20 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﺑﻪ ﮐﻪ ﺻﻮرت ﮔﺎواژ ﻋﺼﺎره را درﯾﺎﻓﺖ ﮐﺮدﻧﺪ. اﺑﺘﺪا ﺗﯿﻮ اﺳﺘﺎﻣﯿﺪ ﺑﻪ ﻣﺪت ﺳﻪ ﻫﻔﺘﻪ و ﻫﻔﺘﻪ اي دو ﺑﺎر ﺑﻪ ﻣﯿﺰان 1ﺳﯽﺳﯽ ﺑﻪ ﺗﻤﺎم ﮔﺮوهﻫﺎ ﺑﻪ ﺟﺰ ﺷﺎﻫﺪ ﺗﺰرﯾﻖ ﮔﺮدﯾﺪ. ﺳﭙﺲ ﺑﻪ ﻣﺪت 14 روز ﮔﺎواژ ﻋﺼﺎره ﻣﺮزه و آوﯾﺸﻦ ﺑ ﮔﺮوه ﻫﺎي ﺗﺤﺖ درﻣﺎن ﺑﺎ ﻋﺼﺎره اﻧﺠﺎم ﮔﺮﻓﺖ و ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل ﻣﺜﺒﺖ ﮔﺎواژ ﮐﭙﺴﻮل ﺳﯿﻠﯽ ﺑﯿﻨﯿﻦ ﺑﻪ ﻣﯿﺰان 8 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﺑ ﻣﺪت 14 روز اﻧﺠﺎم ﮔﺮﻓﺖ. ﺳﭙﺲ ﻧﻤﻮﻧﻪ ﺧﻮن و ﮐﺒﺪ از ﻣﻮش ﻫﺎ ﺗﻬﯿﻪ ﺷﺪ. ﻣﻄﺎﻟﻌﺎت ﻫﯿﺴﺘﻮﭘﺎﺗﻮﻟﻮژﯾﮑﯽ ﺑﯿﺎﻧﮕﺮ اﯾﻦ ﻣﻄﻠﺐ اﺳﺖ ﮐ ﺑﯿﺸﺘﺮﯾﻦ ﺿﺎﯾﻌﺎت ﮐﺒﺪي در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﺷﺎﻫﺪ ﻣﺮﺑﻮط ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل ﻣﻨﻔﯽ ﻣﯽ ﺑﺎﺷﺪ ﻫﻤﭽﻨﯿﻦ ﻣﺸﺨﺺ ﺷﺪ ﺑﺎ اﻓﺰاﯾﺶ ﻣﻘﺪار دوز ﻣﺼﺮﻓﯽ ﻋﺼﺎره، از ﻣﯿﺰان آﻧﺰﯾﻢﻫﺎي ﮐﺒﺪي آﺳﭙﺎرﺗﺎت آﻣﯿﻨﻮﺗﺮاﻧﺴﻔﺮاز و آﻻﻧﯿﻦ آﻣﯿﻨﻮﺗﺮاﻧﺴﻔﺮاز و آﺳﯿﺐﻫﺎي ﭘﺎﺗﻮﻟﻮژﯾﮑﯽ ﮐﺒﺪﮐﺎﺳﺘ ﺷﺪه اﺳﺖ. ﺑﺎ ﺑﺮرﺳﯽ ﻧﺘﺎﯾﺞ ﭼﻨﯿﻦ ﺑﻪ ﻧﻈﺮ ﻣﯽرﺳﺪ وﺟﻮد ﺗﺮﮐﯿﺒﺎت ﻓﻼوﻧﻮﺋﯿﺪي و ﻓﻨﻠﯽ در ﮔﯿﺎه آوﯾﺸﻦ دﻧﺎﯾﯽ و ﻣﺮزه ﺑﺨﺘﯿﺎري ﻣﯽ ﺗﻮاﻧﺪ ﻣﻨﺠﺮ ﺑﻪ ﮐﺎﻫﺶ اﺛﺮات ﺳﻤﯽ اﻟﻘﺎء ﺷﺪه ﺗﻮﺳﻂ ﺗﯿﻮاﺳﺘﺎﻣﯿﺪ در ﺑﺎﻓﺖ ﮐﺒﺪ ﻣﻮش ﺷﻮد.

nowadays, one of the problems of human societies is liver diseases and using chemical drugs with their side effects, causing the researchers change their approaches towards new sources of herbal drugs .The aim of this study was to investigate the effect of Satureja bachtiarica and Thymus daenesis Celak the liver damage is caused by injection thioacetamide. 54 male wistar rats were randomly selected and divided into 9 groups of 6 each groups the (first sahed, second of positive control, third of negative control, fourth, fifth, sixth extracts of Satureja bachtiarica with 5, 10 and 20 miligram per kilogram ,seven , eight and nine extract of Thymus daenensis Celak with 5, 10 and 20 mg/kg extract were given by gavage.Thioacetamide first for three week and twice weekly a rats of 1cc to all groups except the control group were injection then gavage for 14 days savory and thyme extract groups were treated with the extract and the positive drug control group treated with 8 mg/kg capsule sibilinin slap for 14 days.then the blood samples were taken and liver of rats. Histopathologic study suggests that liver lesions compared to the control group is related to the negative control group .Aspartate aminotransferase and alanine aminotransferase well as in the study were assessed and showed that extracts of thyme daenensis has a positive effect on liver fibrosis. By studying the findings, it seems that Flavonoid compounds and phenolic in Thymus daenesis Celak and Satureja bachtiarica can decrease the toxic effects induced By thioacetamide In liver tissue of Mice.