بررسي اثر عصاره زغال اخته (Cornus mas) بر ترميم زخم پوستي رت نر ديابتي نژاد ويستار

The Effect of Cornus mas Extract on the Wound Healing of the Diabetic Male Wistar Rats

ﺑﯿﻤﺎري دﯾﺎﺑﺖ ﯾﮑﯽ از ﻋﻠﻞ ﺗﺎﺧﯿﺮ در ﺗﺮﻣﯿﻢ زﺧﻢ اﺳﺖ. از آﻧﺠﺎ ﮐﻪ ﻣﯿﻮه زﻏﺎل اﺧﺘﻪ )Cornus mas داراي ﻣﻘـﺎدﯾﺮ زﯾـﺎدي آﻧﺘـﯽ اﮐﺴﯿﺪان ﻣﯽﺑﺎﺷﺪ، در اﯾﻦ ﺗﺤﻘﯿﻖ اﺛﺮ ﻋﺼﺎرهي اﯾﻦ ﻣﯿﻮه ﺑﺮ روﻧﺪ ﺗﺮﻣﯿﻢ زﺧﻢ رت ﻧﺮ دﯾﺎﺑﺘﯽ ﻧﮋاد وﯾﺴﺘﺎر ﻣﻮرد ﺑﺮرﺳـﯽ ﻗـﺮار ﮔﺮﻓﺘـﻪ اﺳﺖ. در اﯾﻦ ﺗﺤﻘﯿﻖ 48 ﺳﺮ رت ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر ﺑﻪ 4 ﮔﺮوه 12 ﺗﺎﯾﯽ ﺗﻘﺴﯿﻢ ﺷﺪ. ﮔﺮوهﻫﺎ ﺷﺎﻣﻞ: 1- ﮔـﺮوه ﮐﻨﺘـﺮل ﯾـﺎ ﺳـﺎ ﻟﻢ ﺑـﺪون درﯾﺎﻓﺖ ﻋﺼﺎره زﻏﺎل اﺧﺘﻪ و اوﺳﺮﯾﻦ ﺑﺮ روي زﺧﻢ، ﺑﻌﻨﻮان ﮔﺮوه ﺳﺎﻟﻢ. 2- ﮔﺮوه ﺷﻢ: ﻣﻮشﻫﺎي دﯾﺎﺑﺘﯽ ﺗﺤـﺖ ﺗﯿﻤـﺎر ﺑـﺎ اوﺳـﺮﯾﻦ روزي 2ﺑﺎر(. 3- ﮔﺮوه ﺗﺠﺮﺑﯽ1: ﻣﻮشﻫﺎي دﯾﺎﺑﺘﯽ ﺑﺪون درﯾﺎﻓﺖ اوﺳﺮﯾﻦ و ﻋﺼﺎره، 4ـ ﮔﺮوه ﺗﺠﺮﺑﯽ2: ﮔﺮوه دﯾﺎﺑﺘﯽ ﺗﺤـﺖ ﺗﯿﻤـﺎر ﺑﺎ ﭘﻤﺎد ﻋﺼﺎره زﻏﺎل اﺧﺘﻪ )روزي2 ﺑﺎر(. ﭘﺲ از دﯾﺎﺑﺘﯽ ﺷﺪن، در ﮔﺮوه ﻫﺎي ﺗﺠﺮﺑﯽ، زﺧﻤﯽ ﺑﻪ ﻃﻮل 3 ﺳـﺎﻧﺘﯽ ﻣﺘـﺮ در ﻧﺎﺣﯿـﻪ ﭘﺸـﺖ رتﻫﺎ اﯾﺠﺎد ﮔﺮدﯾﺪ. ﻃﻮل زﺧﻢﻫﺎ ﺗﺎ 21 روز ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻧﺘﺎﯾﺞ ﻣﻘﺎﯾﺴﻪ ﻣﯿﺎﻧﮕﯿﻦ ﻃﻮل زﺧﻢﻫﺎ در ﮔـﺮوه ﻫـﺎي ﺑﺮرﺳـﯽ ﺷﺪه ﻧﺸﺎﻧﮕﺮ اﯾﻦ اﺳﺖ ﮐﻪ ﮔﺮوه ﺗﺠﺮﺑﯽ 2 ﮐﺎﻫﺶ ﻣﻌﻨﺎداري در ﺣﺪ 0/01 ≤ p ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوهﻫﺎي ﺷـﻢ و ﺗﺠﺮﺑـﯽ 1 داﺷـﺘﻪ اﺳـﺖ زﻏﺎل اﺧﺘﻪ از ﻃﺮﯾﻖ ﮐﺎﻫﺶ ﮔﻠﻮﮐﺰ ﺧﻮن و اﺻﻼح ﺑﺮﺧﯽ ﻓﺎﮐﺘﻮرﻫﺎي ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ، آﺛﺎر درﻣﺎﻧﯽ در ﺑﯿﻤﺎري دﯾﺎﺑﺖ اﯾﺠﺎد ﻣـﯽ ﮐﻨـﺪ. ﺑـﺮ اﺳﺎس ﯾﺎﻓﺘﻪﻫﺎي ﺑﺪﺳﺖ آﻣﺪه در اﯾﻦ ﺗﺤﻘﯿﻖ ﭘﻤﺎد زﻏﺎل اﺧﺘﻪ ﻣﯽﺗﻮاﻧﺪ ﻣﻮﺟﺐ ﺗﺴﺮﯾﻊ در ﺗﺮﻣﯿﻢ زﺧﻢﻫﺎي ﭘﻮﺳﺘﯽ ﻧﻤﻮﻧﻪﻫﺎي ﺳـﺎﻟﻢ و دﯾﺎﺑﺘﯽ ﺷﻮد

Diabetes is one of the reasons for delayed wound healing; as the fruit of blueberries, Cornus mas, possesses high content of antioxidants, therefore, this study aims to investigate the extract of this fruit on wound healing in a male rat of Wistar strain affected by diabetes.In this study, 48 male rats of Wistar strain were divided into 4 groups of 12. After affecting these groups to diabetes (except control group), there was created a wound at the back of the rat in all 4 groups; the length of the wounds in the groups were investigated up to 21 days; (1) control group: with no blueberries extract and ocerin treated on their wounds, called as healthy group; (2) Shem Group: they include a group of diabetics rats who were treated by ocerin (twice per day); (3) Experimental Group 1: they included a group of diabetics rats who received no ocerin and extract; (4) Experimental Group 2: they include the diabetics group who treated by the ointment of blueberries extract (twice a day).Results of comparing the mean of wounds length in the investigated groups indicate that the experimental group 2 had significant reduction up to p ≤ 0.01 than Shem and experimental1 groups. By reducing the blood glucose and modifying some biochemical factors, blueberries makes treatment effect on diabetes. According to the results of this study, blueberries ointment may accelerate skin wound healing in the healthy and diabetic samples.