اثر منابع مختلف نيتروژني بر ميزان زيتوده و محتوي پروتئيني جلبك سبز- آبي اسپيرولينا پلاتنسيس (Spirulina platensis)

The effect of different Nitrogen sources on growth rate and protein content of Spirulina platensis

نيتروژن به عنوان يكي از عناصر مهم موثر بر متابوليسم ليپيد و پروتئين در ريزجلبك‌ها شناخته شده است. در اين پژوهش به منظور بهينه‌سازي ميزان زيتوده جلبك Spirulina platensis و همچنين ميزان پروتئين آن، از منابع مختلف نيتروژني همچون KNO3،NH4NO3 و اوره استفاده گرديد. جلبك اسپيرولينا در محيط كشت زاروك محتوي منابع مختلف نيتروژن در ارلن‌هاي 3 ليتري محتوي 5/2 ليتر آب درياي استريل شده تحت شرايط يكسان با درجه‌حرارت °C25-22، شوري ppt 30، قليائيت 8 و دوره نوريh 12:12 (تاريكي: روشنايي) كشت گرديد. مطابق با نتايج، جلبك اسپيرولينا بطور موفقيت‌آميزي در محيط كشت زاروك حاوي منابع مختلف نيتروژني رشد نمود و با وجوديكه بيشينه زيتوده جلبكي در محيط كشت حاوي NH4NO3 بدست آمد، ولي اختلاف معني‌داري با ساير منابع نيتروژني نشان نداده است (05/0

Nitrogen is known to have a strong influence on the metabolism of lipids and protein in various microalgae. In the present study, the production of S. platensis was optimized in terms of biomass and protein by using different Nitrogen sources as NH4Cl, NH4NO3 and Urea. S. platensis was grown in Zarrouk’s medium in a 3000 ml Erlenmeyer flask, in which the NaNO3 was replaced by NH4Cl, NH4NO3 and Urea with concentrations of 0.010, 0.025 and 0.050 M. Cultures were incubated at temperature of 30oC, salinity of 25 ppt and initial pH of 9.5 under 12/12 hour light-dark photo period with normal white light. The results clearly showed that S. platensis successfully cultivated by using different Nitrogen regimes, and though the maximum biomass was produced in medium containing NH4NO3, but there is not significant differences between treatments (p>0.05). The maximum protein content was obtained in culture containing NH4NO3 followed by NH4Cl and KNO3, and there is not significant differences between treatments (p>0.05). Moreover, in all S. platensis cultures, increasing in nitrogen concentrations, led to an increase in maximum biomass and protein content. The chl. content increased with increasing Nitrogen concentrations in all treatments and relatively high values (9.18 µg/ml) were found with KNO3 as a Nitrogen source at 14th day of culturing period. Overall, though the results of present study clearly showed no significant differences between treatments, but using of NH4NO3 could have relatively more effectiveness than the other Nitrogen sources.