ارزيابي تراكم عروق خوني كوچك با استفاده از ماركرCD34 دركارسينوم سلول سنگفرشي سر و گردن

Immunohistochemical evaluation of angiogenesis in head and neck squamous cell carcinoma. Using CD34 Marker

سابقه و هدف: آنژيوژنز در رشد و گسترش تومورهاي بدخيم و همچنين مشي تومورنقش محوري دارد. با توجه به اينكه كارسينوم سلول سنگفرشي سر و گردن (SCC) شايعترين بدخيمي حفره دهان است , مطالعه نقش آنژيوژنز در تعيين مشي SCCاز اهميت بسزايي برخوردار است . هدف از اين مطالعه بررسي رابطه بين تراكم عروقي خوني كوچك (MVD) با فاكتورهاي دموگرافيك بيماران درجه تمايز اين تومور بود. مواد و روش ها: دراين مطالعه توصيفي, تحليلي تعداد 22 بلوك كارسينوم سلول سنگفرشي سروگردن از آرشيو بخش آسيب شناسي دهان, فك و صورت دانشكده دندانپزشكي قزوين استخراج شد و grading لامهاي هماتوكسيلين و ائوزين آنها طبق طبقه بندي Broderتوسط پاتولوژيست انجام شد. بلوكها به روش ايمنوهيستوشيمي با آنتي بادي CD34 رنگ آميزي شدند. در هر نمونه, سه ناحيه با رنگ پذيري شديد انتخاب و ميانگين MVD انجام شد. شمارش و محاسبه شد. آناليز آماري داده ها با آزمونهاي Anovaو T Student با سطح معني داري 0/05 انجام شد. يافته‌ها: ميزان تراكم عروقي در انواع Well diff SCC برابر با 7/4±32/66 ودر انواعdiff Moderate -Poor برابر با 7/1±43/2 بود (0/003=P) فاكتورهاي دموگرافيك بيماران ارتباط معني داري با ميزان تراكم عروقي نشان ندادند. (0/05

Background and Aim: Angiogenesis has a significant role in growth and development, progression and behavior of malignant tumors. Since SCC is the most common carcinoma of oral cavity, determination of angiogenesis role in behavior of this malignancy is of great importance. The purpose of this study was to evaluate the relationship between MVD and demographic factors such as age, gender and location and histopathologic grade of the tumor. Method and Material: In this descriptive-analytic study, 22 paraffin embedded blocks of SCC of head and neck region. From oral & Maxillofacial pathology dept., Dental faculty, Qazvin University. H &E stained slides of related patients were selected and the specimens were graded by pathologist according to Broder’s classification. The blocks were then stained with anti CD34 monoclonal antibody by means of immunohistochemistry method. In each specimen, three highly stained fields were selected and then MVD average of those fields were counted and analyzed. Analysis of variance (ANOVA) and T-Student analysis were then used to measure the variables with p<0.05 as level of significance. Results: MVD was 32.66±7.4 and 43.2±7.1 in well and moderate-poor diff SCC samples (p=0.003) These was no significant correlation between demographic factors and MVD (P>0.05). Conclusion: According to this study, there was a reverse relation between degree of differentiation of SCC and angiogenesis. Hence it seems in low grade tumors-in addition to better cell differentiation-less angiogenesis similarly plays role in prognosis of these lesions.