پديد آورندگان :
حاجبي، سحر دانشگاه آزاد اسلامي، واحد مشهد، مشهد، ايران , همايوني تبريزي، مسعود دانشگاه آزاد اسلامي، واحد مشهد، مشهد، ايران , نخعي مقدم، محبوبه دانشگاه آزاد اسلامي، واحد مشهد، مشهد، ايران
چكيده فارسي :
پيش زمينه و هدف
در اين مطالعه از عصاره گرده گياه كلزا براي سنتز نانوذره نقره به روش سبز استفاده شد و مهار رگزايي و سميت سلولي آن موردسنجش قرار گرفت.
مواد و روش ها
سنجش سميت سلولي توسط روش MTT بر سلول هاي نرمال رده Huvec و سلول هاي سرطان كبد رده HepG2 و سلول هاي سرطان كولون رده HT-29 با طراحي يك مطالعه آزمايشگاهي (test-tub Lab) انجام شد. همچنين ارزيابي آنژيوژنز توسط روش CAM بر روي تخم مرغ هاي نطفه دار موردمطالعه قرار گرفت.
يافته ها
نتايج سنجش سميت نانوذره نقره سنتز شده نشان مي دهد اين نانوذره در غلظت هاي بسيار پايين به صورت معني داري توانايي مهار سلول هاي سرطاني كبد و كولون را دارد (0.001 ≤ p). در 24 ساعت IC50 سلول هاي سرطاني كبد و كولون به ترتيب حدود 0.3 و 6 ميكروگرم بر ميلي ليتر مي باشد. اين غلظت از نانوذره روي سلول هاي نرمال اثر كشندگي ندارد IC50 آن 0.5 ميكروگرم بر ميلي ليتر است. نتايج تست CAM نشان داد كه نانوذره در غلظت هاي 100 و 200 ميكروگرم بر ميلي ليتر باعث كاهش قابل توجهي در ميانگين تعداد عروق، انشعابات عروق، كاهش ميزان قد و وزن جنين ها در قياس با جنين هاي شاهد مي شود (0.05≤ p).
نتيجه گيري
نانو ذرات در غلظت هاي پايين سلول هاي سرطاني كبد و كولون را مهار مي نمايد. همين موضوع بيان مي كند اين نانوذره گزينه اي مناسب براي مهار كردن سلول هاي سرطاني باشد. اين نانوذره داراي توانايي هاي زيادي در رابطه با مهار رگزايي مي باشد.
چكيده لاتين :
Background & Aims: Angiogenesis is an active reaction. In this study, the extract of Rapeseed Flower
Pollen with different antioxidant properties was used to prepare the Bio-green Silver nanoparticle and
then its anti -angiogenic effects of was evaluated. The Bio-green synthesized nanoparticles that were
synthesized by the plant extract have the potential to be used as a reducing agent for metal ions to metal
molecules present in the composition. Today, green nanoparticles are used extensively due to biological
properties such as antioxidant properties, angiogenesis inhibitors, and cancer control.
Materials & Methods: In this test-tube lab research, silver nanoparticles made from rapeseed pollen
were used to investigate anti-angiogenic effects using a CAM assay. For this purpose, 60 eggs (Ross)
were purchased and randomly assigned into 6 groups (n=10): a control group, a laboratory control, and
four treatment groups (25, 50, 100, 200μg/ml). On the second day, the incubation window was made
and on the eighth day, samples were treated with different concentrations of nanoparticles. On the
twelfth day, the photographs were taken from the samples, then the number and the length of the vessels
were evaluated using Imag J software and the height and weight of the embryos were measured. Data
were analyzed by SPSS software and ANOVA test and P <0.05 was considered significant. The MTT
assay was used to evaluate the toxicity of this nanoparticle against liver cancer (HT-29) and (HepG2)
and normal cell (Huvec).
Results: The results of this experiment showed that the nanoparticle are significantly capable of
decreasing the length and the number of blood vessels at concentrations of 100 and 200 μg / ml (p≤0.05).
They are also able to reduce the length and the weight of the treated embryos in comparison to the
control group. The results showed that the silver nanoparticles can significantly inhibit liver cancer cell
(HT-29) and HepG2 (IC50: 0.15 μg/ml) in very low concentrations (IC50:6 μg/ml) but does not have an
inhibitory effect in similar concentration on normal cells (Huvec) (IC50: 0.6 μg/ml) (p ≤ 0.001).
Conclusion: The results showed that the synthesized nanoparticle from rapeseed pollen has an inhibitory
effect on the rate of angiogenesis which makes this nanoparticle a suitable candidate for cancer
treatment. It does not have toxic effects in similar concentrations on Huvec cells, but it is also able to
inhibit the HT-29 and HepG2 cells in a low concentration.
