ارتباط پلي مورفيسم XRCC1 (rs25487) و آسيب‌پذيري افراد شاغل در صنايع شيميايي نسبت به بنزن

The association between polymorphism XRCC1 (rs25487) and the susceptibility of chemical industry workers to benzene

زمينه و هدف: بنزن به‌عنوان يك تركيب سرطان‌زا، با توليد راديكال‌هاي آزاد اكسيژن سبب آسيب به DNA مي‌شود. اثرات بنزن در سيستم خوني گزارش شده است. به نظر مي‌رسد ژن XRCC1 به‌عنوان يك ژن مهم در سيستم ترميم بازهاي آسيب‌ديده، در حساسيت افراد نسبت به بنزن تأثيرگذار باشد. هدف از اين مطالعه بررسي ارتباط پلي مورفيسم 25487rs در ژن XRCC1 و آسيب‌پذيري افراد شاغل در صنايع شيميايي در برابر بنزن است. مواد و روش‌ها: در اين مطالعه مورد شاهدي، 60 فرد مورد و 60 فرد شاهد كه در مواجهه 2 سال متوالي با بنزن بودند، موردبررسي قرار گرفتند. افرادي كه هيچ تغييري در پارامترهاي خوني نداشتند به‌عنوان گروه شاهد و افرادي كه لنفوسيت خارج از محدوده طبيعي داشتند به‌عنوان گروه مورد انتخاب شدند. نمونه‌هاي خون از كاركنان صنايع شيميايي جمع‌آوري شد. پلي مورفيسم ژنتيكي با روش RFLP-PCR با استفاده از آنزيم 1MSP تعيين گرديد. نتايج: اختلاف معني‌داري بين فركانس آللي A و G ديده نشد (05/0

Background & Objective: Benzene, as a carcinogenic compound, can damage DNA by producing free oxygen radicals. Benzene effects have been reported in the blood system. It seems that XRCC1 gene, as a gene involved in the repair of damaged bases, plays a role in the sensitivity of individuals to benzene. The purpose of this study was to investigate the association between rs25487 polymorphism in XRCC1 gene and the susceptibility of chemical industry workers against benzene. Material & Methods: In this case-control study, 60 cases and 60 controls who were exposed to benzene for 2 consecutive years were examined. People who did not have any changes in blood parameters were selected as the control group and those who have shown lymphocytes outside the normal range were considered as a case group. Blood samples were collected from chemical workers. Gene polymorphism was determined by RFLP-PCR using MSP1enzym. Results: There was no significant difference between allelic frequencies A and G (P >0.05). No significant association was found between XRCC1 polymorphism and benzene susceptibility and lymphocytic abnormalities (OR: 1.43, 95% CI (0.47 - 4.31), P = 0.52). Conclusion: It seems that rs25487 polymorphism in the XRCC1 gene does not play a role in the sensitivity of individuals to benzene. Of course, due to the role of XRCC1 gene in response to DNA damage, other polymorphisms of this gene and polymorphism that are targeted in this study are evaluated at a wider level.