مقايسه اثر تمرينات تناوبي شديد و تداومي با شدت متوسط بر بيان ژن NF-κB و IL-6 در ريه موش ‌هاي صحرايي نر پير و جوان

Comparison of the Effect of High Intensity Interval Training and Moderate Intensity Continuous Training on Gene Expression of Il-6 and Nf-Κb in Lung Tissue of Old and Young Male Rats

مقدمه: افزايش سن با روند افزايش التهاب در بافت هاي بدن همراه مي شود كه بافت ريه را نيز متاثر مي­ كند. هدف از پژوهش حاضر مقايسه اثرات دو شيوه­ تمرين تناوبي شديد و تداومي شدت متوسط بر بيان ژن NF-κB و IL-6 در بافت ريه موش ­هاي پير و جوان مي ­باشد. مواد و روش ­ها: چهل و دو سر موش نر نژاد ويستار جوان و پير به 6 گروه شامل كنترل جوان، كنترل پير، تمرين تداومي جوان، تمرين تداومي پير، تمرين تناوبي شديد جوان و تمرين تناوبي شديد پير تقسيم شدند. پروتكل تمريني شامل 6 هفته، 5 روز در هفته تمرينات تناوبي شديد و تمرينات تداومي شدت متوسط بود. جهت اندازه­ گيري بيان ژن ­هاي NF-κB و IL-6 ريه­ ها از روش Real Time-PCR و آزمون آماري تحليل واريانس يك راهه همراه با تست تعقيبي شفه استفاده گرديد(P<0.05). يافته­ هاي پژوهش: اختلاف معناداري در بيان ژن NF-κB بين گروه كنترل پير و تمرين تداومي پير(P<0.001) و بين گروه كنترل پير و تمرين تناوبي شديد پير(P<0.001) و بين گروه تمرين تداومي پير و تمرين تناوبي شديد پير(P=0.007) وجود داشت. به علاوه اختلاف معناداري در بيان ژن IL-6 بين گروه كنترل پير و تمرين تداومي پير(P<0.001) و بين گروه كنترل پير و تمرين تناوبي شديد پير(P=0.006) مشاهده شد. هم چنين اختلاف معناداري در بيان ژن IL-6 بين گروه تمرين تناوبي شديد جوان و تمرين تناوبي شديد پير مشاهده شد(P=0.035). بحث و نتيجه­ گيري: هر دو شيوه تمرينات تناوبي شديد و تمرينات تداومي شدت متوسط مي ­توانند موجب كاهش فاكتورهاي التهابي در ريه هنگام پيري شوند و از بروز اختلالات و بيماري ­هاي ناشي از پيري جلوگيري كنند. با اين حال به نظر مي­ رسدكه تمرينات تداومي شدت متوسط موثرتر از تمرينات تناوبي شديد باشد.

Introduction: Aging is accompanied by an increase in inflammation in the tissues of the body, which also causes lung inflammation. This study aimed to compare the effect of high-intensity interval training and moderate-intensity continuous training on gene expression of NF-κB and IL-6 in lung tissue of old and young male rats. Materials & Methods: In total, 42 young and old male Wistar rats were randomly divided into 6 groups, namely young control, old control, young continuous training, old continuous training, young high-intensity interval training, and old high-intensity interval training. The training protocol included high-intensity interval training and moderate-intensity continuous training during six-week (5 sessions per week). The gene expression of NF-κB and IL-6 were evaluated using a real-time polymerase chain reaction technique, and the data were analyzed through a one-way analysis of variance and the Scheffe post hoc test (P<0.05). Ethics code: IR.ut.Rec.1395005 Findings: There were significant differences between old control and old continuous training (P<0.001), old control and old high-intensity interval training (P<0.001), as well as old continuous training and old high-intensity interval training groups (P=0.007) regarding the gene expression of NF-κB. Moreover, significant differences were observed between old control and old continuous training (P<0.001), as well as old control and old high-intensity interval training groups (P=0.006) in terms of the gene expression of IL-6. In addition, there was a significant difference between young high-intensity interval training and old high-intensity interval training groups (P=0.035) regarding the gene expression of IL-6. Discussion & Conclusions: According to the results, both high-intensity interval training and moderate-intensity continuous training can result in the prevention of the incidence of aging-related diseases and disorders as well as the reduction of inflammatory factors in lung tissue in aged rats. However, it seems that moderate-intensity continuous training is more effective than high-intensity interval training.