تأثير تمرين استقامتي كوتاه مدت روي مسير سيگنالينگ PGC-1α/FNDC5/BDNF در رت‌هاي پاركينسوني 6-OHDA

The Effect of Short Endurance Training on PGC-1α/FNDC5/BDNF Signalling Pathway In 6-OHDA-Induced Parkinson’s Rats

زﻣﯿﻨﻪ و ﻫﺪف: ﺑﯿﻤﺎري ﭘﺎرﮐﯿﻨﺴﻮن ﯾﮏ اﺧﺘﻼل ﺗﺨﺮﯾﺐ ﻧﻮروﻧﯽ اﺳﺖ ﮐﻪ ﺑﻪ وﺳﯿﻠﻪ ﻧﻘﺺ رﻓﺘﺎري، ﺷﻨﺎﺧﺘﯽ و ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ ﻣﺸﺨﺺ ﻣﯽ ﺷﻮد. اﯾﻦ ﻣﻄﺎﻟﻌﻪ اﺛﺮ ﺗﻤﺮﯾﻦ اﺳﺘﻘﺎﻣﺘﯽ ﻗﺒﻞ از ﺗﺰرﯾﻖ OHDA-6 را ﺑﺮ ﺑﯿﺎنFNDC5 ،PGC-1α و BDNF ﺑﺮرﺳﯽ ﻣﯽﮐﻨﺪ. ﻣﻮاد و روشﻫﺎ: در ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ، رت ﻫﺎي ﭘﺎرﮐﯿﻨﺴﻮﻧﯽ از ﻃﺮﯾﻖ ﺗﺰرﯾﻖOHDA -6 ﺑﻪ ﻣﯿﺰان 8􀀁g در دﺳﺘﻪ ﻣﯿﺎﻧﯽ ﻣﻐﺰ ﻗﺪاﻣﯽ ﺑﺎ اﺳﺘﻔﺎده از دﺳﺘﮕﺎه اﺳﺘﺮﯾﻮﺗﺎﮐﺲ اﯾﺠﺎد ﺷﺪﻧﺪ. ﮔﺮوه ﻫﺎي آزﻣﺎﯾﺸﯽ ﺷﺎﻣﻞ 1. ﺳﺎﻟﯿﻦ، 2. OHDA-6، 3. ﺳﺎﻟﯿﻦ + ﺗﻤﺮﯾﻦ و 4. OHDA-6 + ﺗﻤﺮﯾﻦ ﺑﻮدﻧﺪ )ﻫﺮ ﮔﺮوه= 8 رت(. ﮔﺮوه ﻫﺎي ﺗﻤﺮﯾﻦ 2 ﻫﻔﺘﻪ ﭘﺲ از ﺟﺮاﺣﯽ، 14 روز ﻣﺘﻮاﻟﯽ دوﯾﺪن روي ﻧﻮارﮔﺮدان را آﻏﺎز ﮐﺮدﻧﺪ. 4 ﻫﻔﺘﻪ ﭘﺲ از ﺗﺤﺮﯾﮏ ﺑﺎOHDA -6/ﺳﺎﻟﯿﻦ ﺑﯿﺎن mRNA ﺑﺮاي FNDC5 ،PGC-1α و BDNF در اﺳﺘﺮﯾﺎﺗﻮم رت ﻫﺎ ﺑﺮرﺳﯽ ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از آﻧﻮاي ﯾﮏ ﻃﺮﻓﻪ ﻧﺸﺎن داد ﮐﻪ ﺗﺰرﯾﻖ OHDA-6 )ﮔﺮوه ﭘﺎرﮐﯿﻨﺴﻮن(، ﺳﺒﺐ اﻓﺰاﯾﺶ ﭼﺮﺧﺶ ﻫﺎي ﻧﺎﻣﺘﻘﺎرن ﻧﺎﺷﯽ از آﭘﻮﻣﻮرﻓﯿﻦ و ﮐﺎﻫﺶ ﻣﻌﻨﺎدار در ﺑﯿﺎن mRNAﻫﺎي ﻣﺴﯿﺮ ﺳﯿﮕﻨﺎﻟﯿﻨﮓ PGC-1α/FNDC5/BDNF ﻣﯽ ﺷﻮد. ﺑﺎ اﯾﻨﺤﺎل، ﺗﻤﺮﯾﻦ اﺳﺘﻘﺎﻣﺘﯽ در ﮔﺮوه ﭘﺎرﮐﯿﻨﺴﻮن+ﺗﻤﺮﯾﻦ، اﯾﻦ اﺧﺘﻼﻻت را ﺑﻪ ﻃﻮر ﻣﻌﻨﺎداري ﮐﺎﻫﺶ ﻣﯽدﻫﺪ)0/05≤p(. ﻧﺘﯿﺠﻪﮔﯿﺮي: ﺑﻪ ﻧﻈﺮ ﻣﯽ رﺳﺪ ﮐﻪ ﺗﻤﺮﯾﻦ اﺳﺘﻘﺎﻣﺘﯽ ﻣﯽ ﺗﻮاﻧﺪ اﺧﺘﻼﻻت رﻓﺘﺎري و ﻓﯿﺰﯾﻮﻟﻮژﯾﮑﯽ را در رﺗﻬﺎي ﭘﺎرﮐﯿﻨﺴﻮﻧﯽ ﮐﺎﻫﺶ دﻫﺪ.

Background&Purpose: Parkinson’s disease is a neurodegenerative disorder that is characterized by impairment on behavioral, cognitive and biochemical. This study investigated the effect of endurance training after the 6-OHDA induction, on PGC-1α, FNDC5 and BDNF expression. Methodology: In this study, Parkinson’s rats were made by 8𝜇g injection of 6-OHDA into the Medial Forebrain Bundle using stereotaxic. Experimental groups were: 1. Saline, 2. 6-OHDA, 3. Saline+training and 4. 6-OHDA+training (n=8). Training groups 2 weeks after surgery started 14 consecutive days treadmill running. 4 weeks after the induction with 6-OHDA/Saline, mRNA expression levels for PGC-1α, FNDC5 and BDNF were measured through one way ANOVA in the striatum of rats. Results: It was found that exposure to the 6-OHDA (6-OHDA group), resulted in increasing in the apomorphine-induced rotational asymmetry and significant decrease in mRNAs expression of PGC-1α/FNDC5/BDNF signalling pathway. However, endurance exercise in 6-OHDA+training group reduces these disorders, significantly (p≤0/05). Conclusion: It seems that the endurance training can reduce behavioral and physiological disorders in Parkinson’s disease