ﺗﺄﺛﯿﺮ ﺗﻤﺮﯾﻦ ﻫﻮازي ﺑﺎ دو ﺷﺪت ﻣﺘﻔﺎوت ﺑﺮ ﺑﺮﺧﯽ از ﻋﻮاﻣﻞ آﻧﮋﯾﻮژﻧﺰي در ﺑﺎﻓﺖ ﮐﻠﯿﻪ ﻣﻮشﻫﺎي ﺻﺤﺮاﯾﯽ دﯾﺎﺑﺘﯽ

Effect of Aerobic Training with Two Different Intensities on Some Angiogenesis Factors in Renal Tissue of Diabetic Rats

زﻣﯿﻨﻪ و ﻫﺪف: در ﻣﺒﺘﻼﯾﺎن ﺑﻪ دﯾﺎﺑﺖ ﺑﻪ وﯾﮋه دﯾﺎﺑﺘﯽﻫﺎﯾﯽ ﮐﻪ ﺑﻪ ﻓﻌﺎﻟﯿﺖ ورزﺷﯽ ﻣﯽﭘﺮدازﻧﺪ، ﻋﻮاﻣﻞ ﺑﺎزدارﻧﺪه آﻧﮋﯾﻮژﻧﺰ ﮐﻨﺘﺮل ﺷﺪه و ﻋﻮاﻣﻞ ﭘﯿﺶ ﺑﺮﻧﺪه آﻧﮋﯾﻮژﻧﺰ ﺗﻘﻮﯾﺖ ﻣﯽﺷﻮد. ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﻣﻘﺎﯾﺴﻪ اﺛﺮات ﺗﻤﺮﯾﻨﺎت اﺳﺘﻘﺎﻣﺘﯽ ﺑﺎ ﺷﺪت ﻣﺘﻮﺳﻂ و ﺑﺎﻻ ﺑﺮ ﺑﺮﺧﯽ ﻓﺎﮐﺘﻮرﻫﺎي ﻣﺤﺮك آﻧﮋﯾﻮژﻧﺰ در ﺑﺎﻓﺖ ﮐﻠﯿﻪ رتﻫﺎي ﻧﺮ دﯾﺎﺑﺘﯽ ﻣﯽﺑﺎﺷﺪ. ﻣﻮاد و روشﻫﺎ: 60 ﺳﺮ ﻣﻮش ﺻﺤﺮاﯾﯽ ﻧﺮ ﺑﻪ 6 ﮔﺮوه )10 ﺳﺮ در ﻫﺮ ﮔﺮوه(: ﮐﻢ ﺗﺤﺮك ﮐﻨﺘﺮل )SC(، دﯾﺎﺑﺘﯽ ﮐﻢ ﺗﺤﺮك )SD(، دﯾﺎﺑﺘﯽ ﺗﻤﺮﯾﻦ ﺑﺎ ﺷﺪت ﻣﺘﻮ ﺳﻂ )DMT(، ﺗﻤﺮﯾﻦ ﺑﺎ ﺷﺪت ﻣﺘﻮ ﺳﻂ ﺳﺎﻟﻢ )MT(، دﯾﺎﺑﺘﯽ ﺗﻤﺮﯾﻦ ﺑﺎ ﺷﺪت ﺑﺎﻻ )DHT( و ﺗﻤﺮﯾﻦ ﺑﺎ ﺷﺪت ﺑﺎﻻ ﺳﺎﻟﻢ )HT( ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﭘﺮوﺗﮑﻞ ﺗﻤﺮﯾﻨﯽ ﺷﺎﻣﻞ 8 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﻫﻮازي ﺑﺎ ﺷﺪت ﻣﺘﻮﺳﻂ و ﺑﺎﻻ، 5 ﺟﻠﺴﻪ در ﻫﻔﺘﻪ و 60 دﻗﯿﻘﻪ در ﻫﺮ ﺟﻠﺴﻪ ﺑﻮد. 48 ﺳــﺎﻋﺖ ﭘﺲ از آﺧﺮﯾﻦ ﺟﻠﺴــﻪ ﺗﻤﺮﯾﻦ، ﻣﻮش ﻫﺎ ﺑﯿﻬﻮش ﺷــﺪﻧﺪ و ﺧﻮنﮔﯿﺮي و ﺑﺮداﺷــﺖ ﺑﺎﻓﺖ ﮐﻠﯿﻪ ﺑﺮاي ﺳــﻨﺠﺶ ﮔﻠﻮﮐﺰ، VEGF وVEGFR1 اﻧﺠﺎم ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ﻫ ﺸﺖ ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﻫﻮازي ﺑﺎ ﺷﺪت ﻣﺘﻮ ﺳﻂ و ﺑﺎﻻ ﺑﺎﻋﺚ ﮐﺎﻫﺶ ﻣﻌﻨﯽدار ﮔﻠﻮﮐﺰ ﺧﻮن ﻣﻮشﻫﺎي دﯾﺎﺑﺘﯽ ﺷﺪ، اﻣﺎ اﯾﻦ ﮐﺎﻫﺶ در ﮔﺮوه DMT ﺑﯿﺸﺘﺮ ﺑﻮد. ﻫﻤﭽﻨﯿﻦ ﻫﺸﺖ ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﻫﻮازي ﺑﺎ ﺷﺪت ﻣﺘﻮﺳﻂ و ﺑﺎﻻ در ﻫﺮ دو ﮔﺮوه دﯾﺎﺑﺘﯽ ﺑﺎﻋﺚ اﻓﺰاﯾﺶ ﻣﻌﻨﯽدار VEGF )ﺑﻪ ﺗﺮﺗﯿﺐ 0/002 = P و 0/0001 =P( ﻧﺴــﺒﺖ ﺑﻪSD ﺷــﺪ وﻟﯽ در ﺧﺼــﻮص VEGFR1 ﻓﻘﻂ ﺑﯿﻦ DHT ﻧﺴــﺒﺖ ﺑﻪ ﮔﺮوهSD ﺗﻔﺎوت ﻣﺸﺎﻫﺪه ﺷﺪ )0/007 =P(. ﻧﺘﯿﺠﻪﮔﯿﺮي: در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﺗﻤﺮﯾﻨﺎت ﻫﻮازي ﺑﺎ ﺷﺪت ﺑﺎﻻ، ﺗﻤﺮﯾﻨﺎت ﻫﻮازي ﺑﺎ ﺷﺪت ﻣﺘﻮﺳﻂ ﻣﻮﺟﺐ ﮐﺎﻫﺶ ﺑﯿﺸﺘﺮ ﺳﻄﻮح ﮔﻠﻮﮐﺰ و اﻓﺰاﯾﺶ VEGF ﻣﯽ ﺷﻮﻧﺪ.

Background and purpose: The angiogenesis inhibitors and the pro angiogenesis factors are enhanced in diabetic patients; especially those are involved in exercise. The purpose of this study was to compare the effects of endurance training with moderate and high intensities on some angiogenesis factors in renal tissue of diabetic rats. Materials and Methods: Sixty male rats were divided into 6 groups (n=10 per group): Sedentary control (SC), Sedentary Diabetic (SD), Diabetic Moderate intensity training (DMT), Healthy Moderate intensity training (MT), Diabetic High intensity training (DHT) and Healthy High intensity training (HT). The exercise protocol performed 8 weeks of moderate and high intensity aerobic training, 5 sessions per week and 60 minutes per session. Results: Eight weeks of moderate and high intensity aerobic training significantly reduced blood glucose in diabetic rats, but this decrease was higher in the DMT group. Also, eight weeks of moderate and high intensity aerobic training in both diabetic groups significantly increased VEGF (P = 0.002 and P = 0.000, respectively) compared to the SD, but for VEGFR1 was observed only between DHT compared to SD (P = 0.007). Conclusion: compared to high intensity aerobic training, moderate aerobic training results in a decrease in the levels of glucose and an increase in VEGF.