اثر دو هفته تمرين تناوبي شديد بر G-CSF، G-CSFR و C-Kit در بافت قلب موشهاي صحرايي نر

The Effect of High Intensity Interval Training on the Levels of Myocardial G-CSF, G-CSFR and C-Kit Proteins in Male Rats

ﺳﺎﺑﻘﻪ و ﻫﺪف: ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﺑﻪ ﻋﻨﻮان ﯾﮏ روش ﺗﻤﺮﯾﻨﯽ ﺟﺪﯾﺪ از ﻃﺮﯾﻖ ﻣﺴﯿﺮﻫﺎي ﻣﺨﺘﻠﻒ ﻣﯽ ﺗﻮاﻧﺪ ﻣﻮﺟﺐ ﻫﺎﯾﭙﺮﺗﺮوﻓﯽ ﻓﯿﺰﯾﻮژﯾﮏ ﻗﻠﺐ و ﻣﺤﺎﻓﻈﺖ ﻗﻠﺒﯽ ﺷﻮد. از اﯾﻨﺮو ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﺗﻌﯿﯿﻦ اﺛﺮ دو ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﺑﺮG-CSFR ،G-CSF و C-Kit در ﺑﺎﻓﺖ ﻗﻠﺐ ﻣﻮﺷﻬﺎي ﺻﺤﺮاﯾﯽ ﻧﺮ ﺑﻮد. ﻣﻮاد و روش ﻫﺎ: ﺑﺮ اﯾﻦ اﺳﺎس 10 ﺳﺮ ﻣﻮش 8 ﻫﻔﺘﻪ اي ﻧﺮ ﺑﺎ ﻧﮋاد وﯾﺴﺘﺎر )ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ وزﻧﯽ 234/75+8/7 ﮔﺮم( در دو ﮔﺮوه ﮐﻨﺘﺮل و ﺗﻤﺮﯾﻦ ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﻓﻌﺎﻟﯿﺖ ﮔﺮوه ﺗﻤﺮﯾﻨﯽ از ﭼﻬﺎر ﺑﺨﺶ ﺷﺎﻣﻞ: ﺑﺨﺶ اول ﺳﻪ روز ﺗﻤﺮﯾﻦ ﻫﺮ روز دو ﺟﻠﺴﻪ و ﻫﺮ ﺟﻠﺴﻪ ﺷﺎﻣﻞ 4 ﺗﻨﺎوب ﺷﺪﯾﺪ دو دﻗﯿﻘﻪ ي ﺑﺎ ﺳﺮﻋﺖ 35 ﺗﺎ 40 ﻣﺘﺮ ﺑﺮ دﻗﯿﻘﻪ و 3 ﺗﻨﺎوب آﻫﺴﺘﻪ 2 دﻗﯿﻘﻪ اي ﺑﺎ ﺳﺮﻋﺖ 25 ﺗﺎ 30 ﻣﺘﺮ ﺑﺮ دﻗﯿﻘﻪ ﺑﯿﻦ دو ﺗﻨﺎوب ﺷﺪﯾﺪ ﺑﻮد. ﺑﺨﺶ دوم: دو روز ﺗﻤﺮﯾﻦ ﻣﺎﻧﻨﺪ ﺑﺨﺶ اول از ﻧﻈﺮ ﺗﻌﺪاد ﺗﻨﺎوب ﻫﺎ ﺑﻮد اﻣﺎ ﺑﺎ اﯾﻦ ﺗﻔﺎوت ﮐﻪ ﺷﺪت ﺗﻨﺎوب ﻫﺎي ﺷﺪﯾﺪ ﺑﻪ 40 ﺗﺎ 45 ﻣﺘﺮ ﺑﺮ دﻗﯿﻘﻪ و ﺗﻨﺎوب ﻫﺎي آﻫﺴﺘﻪ ﺑﻪ 28 ﺗﺎ 32 ﻣﺘﺮ ﺑﺮ دﻗﯿﻘﻪ رﺳﯿﺪ. ﺑﺨﺶ ﺳﻮم ﻧﯿﺰ ﺷﺎﻣﻞ ﺳﻪ روز ﺗﻤﺮﯾﻨﯽ ﺑﻮد. ﺗﻌﺪاد ﺗﻨﺎوب ﻫﺎ در اﯾﻦ ﺑﺨﺶ ﺑﻪ 5 ﺗﻨﺎوب ﺷﺪﯾﺪ و 4 ﺗﻨﺎوب آﻫﺴﺘﻪ ﺑﺎ ﺷﺪت ﺑﺨﺶ دوم اﻧﺠﺎم ﺷﺪ. ﺑﺨﺶ ﭼﻬﺎرم ﺷﺎﻣﻞ دو روز ﺗﻤﺮﯾﻨﯽ ﻫﻤﺎﻧﻨﺪ ﺑﺨﺶ ﺳﻮم اﻣﺎ ﺑﺎ اﯾﻦ ﺗﻔﺎوت ﮐﻪ ﺗﻌﺪاد ﺗﻨﺎوب ﻫﺎي ﺷﺪﯾﺪ و آﻫﺴﺘﻪ ﯾﮏ ﺗﻨﺎوب اﻓﺰاﯾﺶ ﯾﺎﻓﺖ. ﭘﺮوﺗﺌﯿﻦ ﻫﺎي G-CSFR ،G-CSF و C-Kit ﺑﺎ روش وﺳﺘﺮن ﺑﻼت در ﺑﺎﻓﺖ ﻗﻠﺐ اﻧﺪازهﮔﯿﺮي ﺷﺪﻧﺪ. ﻧﺘﺎﯾﺞ: آزﻣﻮن t ﻣﺴﺘﻘﻞ ﻧﺸﺎن داد ﮐﻪ ﻣﻘﺎدﯾﺮ G-CSF و C-Kit در ﮔﺮوه ﺗﻤﺮﯾﻨﯽ ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل اﻓﺰاﯾﺶ ﻣﻌﻨﯽ داري داﺷﺖ)0/05≤P(. اﻣﺎ در ﻣﻘﺎدﯾﺮ G-CSFR ﺗﻔﺎوﺗﯽ ﻣﻌﻨﯽ داري ﺑﯿﻦ دو ﮔﺮوه ﻣﺸﺎﻫﺪه ﻧﺸﺪ. ﻧﺘﯿﺠﻪ ﮔﯿﺮي: ﺗﻤﺮﯾﻨﺎت ورزﺷﯽ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﺣﺘﯽ در دوره ﮐﻮﺗﺎه ﻣﺪت ﺑﺎ اﻓﺰاﯾﺶ ﺳﻄﺢ G-CSF و C-Kit ﻣﯽ ﺗﻮاﻧﺪ ﺗﺸﮑﯿﻞ ﻣﯿﻮﺳﯿﺖﻫﺎي ﺟﺪﯾﺪ را ﺗﺤﺮﯾﮏ ﮐﻨﺪ و از اﯾﻦ ﻃﺮﯾﻖ ﻣﺴﯿﺮ ﻫﺎﯾﭙﺮﺗﺮوﻓﯽ ﻓﯿﺰﯾﻮﻟﻮژﯾﮏ ﻗﻠﺐ و ﺳﺎزو و ﮐﺎر ﻣﺤﺎﻓﻈﺘﯽ ﻗﻠﺐ را در ﺑﺮاﺑﺮ آﺳﯿﺐ ﻫﺎي اﺣﺘﻤﺎﻟﯽ ﻓﻌﺎل ﮐﻨﺪ.

Background & Purpose: High intensity training as a new training method can cause physiological cardiac hypertrophy and cardio protection by different pathway. Therefore, the aim of this study was to determine the effect of two weeks of high intensity training on G-CSF, G-CSFR (CD114) and C-kit proteins in the myocardial of male rats. Methodology: 10 male Wistar rats (weight 23.75 ± 8.7 gr) were divided into two groups of control and training. The training group performed two weeks of high intensity training in four parts. First part included three days and every day were two training sessions and every session were 4 repetition two-minutes with speed of 35-40 m/min and three slow repetition were with speed of 25-30 m/min between two intense repetitions. Second part: two days training similar the first part in the repetitions number, but with the difference that intensity repetition increased to 40-45 m/min and slow repetition to 28-32 m/min. The third part included three days of training; the number of repetitions in this session was made up to 5-intensity repetition and 4 slow repetition with intensity of the second part. The fourth part included of two days of training, similar to the third part, but with the difference that number intensity and slow repetitions, one repetition increased. G-CSF, G-CSFR and C-Kit proteins were measured by western blot method. Results: Independent t-test showed that there was a significant increase in G-CSF and C-Kit in the training group (P≥0.05). But, not significant difference in G-CSFR was shown between two groups. Conclusion: Therefore, it seems that even a short period of high intensity training with the increase of G-CSF and C-Kit levels can stimulate the formation of new myositis and this way activated the pathway of physiological cardiac hypertrophy and cardio protection.