اثر هشت هفته تمرين تركيبي (هوازي- مقاومتي) همراه با مصرف مكمل كوآنزيم Q10 بر فعاليت آنزيم‌هاي آنتي‌اكسيداني و شاخص پراكسيداسيون ليپيدي در زنان مبتلا به مولتيپل اسكلروزيس با چرخه جنسي منظم

The Effect of Combined Exercise (Aerobic-Resistance) with Consumption of Coenzyme Q10 on Antioxidant Enzyme Activities and Lipid Peroxidation in Female with Multiple Sclerosis sexual cycle

زﻣﯿﻨﻪ و ﻫﺪف: ﺑﺨﻮﺑﯽ ﻣﺸﺨﺺ ﺷﺪه ﮐﻪ اﺳﺘﺮساﮐﺴﯿﺪاﺗﯿﻮ در ﻣﻮﻟﺘﯿﭙﻞاﺳﮑﻠﺮوزﯾﺲ(MS) ﺳﺒﺐ آﺳﯿﺐ ﻏﺸﺎ ﻟﯿﭙﯿﺪي و ﺗﺨﺮﯾﺐ ﺑﺎﻓﺖ ﻋﺼﺒﯽ ﻣﯽﺷﻮد. ﻫﺪف ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ، ﺑﺮرﺳﯽ اﺛﺮ ﻫﺸﺖ ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﺗﺮﮐﯿﺒﯽ )ﻫﻮازي- ﻣﻘﺎوﻣﺘﯽ( ﻫﻤﺮاه ﺑﺎ ﻣﺼﺮف ﻣﮑﻤﻞ ﮐﻮآﻧﺰﯾﻢ Q10 ﺑﺮ ﻋﻤﻠﮑﺮد آﻧﺰﯾﻢﻫﺎي آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ و ﺷﺎﺧﺺ ﭘﺮاﮐﺴﯿﺪاﺳﯿﻮن ﻟﯿﭙﯿﺪي در زﻧﺎن ﻣﺒﺘﻼ ﺑﻪ MS ﺑﻮد. ﻣﻮاد و روشﻫﺎ: 60زن داوﻃﻠﺐ ﻣﺒﺘﻼ ﺑﻪ MS اﻧﺘﺨﺎب (ﻣﯿﺎﻧﮕﯿﻦ ﺳﻨﯽ 15/9± 35/19 ﺳﺎل، وزن 59/2±13/6 ﮐﯿﻠﻮﮔﺮم و ﻣﻘﯿﺎس ﻧﺎﺗﻮاﻧﯽ 0/6 ±3/5) و ﺑﻪﻃﻮر ﺗﺼﺎدﻓﯽ در 4 ﮔﺮوه ﻣﺴﺎوي داروﻧﻤﺎ، ﻣﮑﻤﻞ، ﺗﻤﺮﯾﻦ-داروﻧﻤﺎ و ﻣﮑﻤﻞ-ﺗﻤﺮﯾﻦ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﮔﺮوهﻫﺎي ﺗﺠﺮﺑﯽ ﮐﻮآﻧﺰﯾﻢ Q10 و ﮔﺮوه ﮐﻨﺘﺮل)داروﻧﻤﺎ(، داروﻧﻤﺎ را ﺑﻪ ﻣﻘﺪار 300 ﻣﯿﻠﯽﮔﺮم در روز ﻣﺼﺮف ﮐﺮدﻧﺪ. ﺑﺮﻧﺎﻣﻪ ﺗﻤﺮﯾﻦ ﺗﺮﮐﯿﺒﯽ )ﻫﻮازي-ﻣﻘﺎوﻣﺘﯽ( ﺑﻪ ﻣﺪت 8 ﻫﻔﺘﻪ، ﺳﻪ ﺟﻠﺴﻪ در ﻫﻔﺘﻪ اﻧﺠﺎم ﺷﺪ. ﻗﺒﻞ و ﺑﻌﺪ از دوره ﺗﻤﺮﯾﻨﯽ، ﺑﺮاي ﺑﺮرﺳﯽ ﺗﻐﯿﯿﺮات آﻧﺰﯾﻢﻫﺎي ﺳﻮﭘﺮاﮐﺴﺎﯾﺪ دﯾﺴﻤﻮﺗﺎز(SOD)، ﮔﻠﻮﺗﺎﺗﯿﻮنﭘﺮاﮐﺴﯿﺪاز (GPX)، ﻇﺮﻓﯿﺖ ﺗﺎم آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ(TAC) و ﻣﯿﺰان ﻣﺎﻟﻮنديآﻟﺪﺋﯿﺪ (MDA) از ﻫﻤﻪ آزﻣﻮدﻧﯽﻫﺎ در ﺣﺎﻟﺖ ﻧﺎﺷﺘﺎ ﺧﻮن ﮔﯿﺮي ﺑﻪ ﻋﻤﻞ آﻣﺪ. ﺑﺮاي ﻣﻘﺎﯾﺴﻪ ﻣﺘﻐﯿﯿﺮﻫﺎ از آزﻣﻮن آﻧﺎﻟﯿﺰ وارﯾﺎﻧﺲ اﻧﺪازهﻫﺎي ﺗﮑﺮاري اﺳﺘﻔﺎده ﺷﺪ. ( 0.05

Background&Purpose: It is well documented that oxidative stress provides neurodegenerative in multiple sclerosis (MS). The purpose of this study was to evaluate the effect of eight week combined exercise (aerobic- resistance) with consumption of coenzyme Q10 on antioxidant enzyme activities and lipid peroxidation in female with multiple sclerosis. Methodology: 60 women with multiple sclerosis (EDSS less than 4, 20-60 years old) were selected and randomly divided in to four equal, (Q10 (n=15), combined training-placebo (n=15), combined training-Q10 (n=15), placebo (n=15)) groups. For 8 weeks, experimental group and Control group consumed Q10 and placebo 300 mg CoQ10 daily, respectively. Subjects in experimental groups performed an aerobic-resistance training program for 8 weeks (3 sessions per week).Fastig blood sample was taken from all subjects before and after performing protocol. For within and between group mean difference comparison, Repeated measures Anova and Tocky were used, respectively (P< 0.05). Result: A repeated measures ANOVA showed that serum level of SOD and Q10 notable increase in experience groups than placebo group (p=0.001).But there was no significant difference in serum level of (MDA, GPX, TAC) between all groups (p>0.05). Conclusion: Although combined training with moderate intensity and coenzyme Q10 could increase serum level of SOD and Q10 in patients with Multiple Sclerosis .but failed to show any significant effects on the antioxidant capacity dnd MDA in woman with Moltiple Sclerosis. We suggest limited consumption of antioxidants supplementation during exercise, to save the adaptive effects of moderate exercise in patients with Multiple Sclerosis.