تأثير يك دوره مصرف مكمل كوئرستين بر بيان ژن 1PGC- در دوران بي‌تحركي در رت‌هاي فعال بعد از يك دوره تمرين HIIT

The Effect of Consumption of Quercetin Supplement on Gene Expression of PGC-1α during Sedentary Period in Active Rats after a HIIT Course

ﻫﺪف از اﯾﻦ ﭘﮋوﻫﺶ، ﺗﻌﯿﯿﻦ اﺛﺮ ﻣﮑﻤﻞ ﮐﻮﺋﺮﺳﺘﯿﻦ در دوران ﺑﯽﺗﺤﺮﮐﯽ ﺑﺮ ﻣﺴﯿﺮ ﺳﯿﮕﻨﺎﻟﯿﻨﮓ 1-PGC اﺳﺖ. ﻫﻤﭽﻨﯿﻦ ﻣﺤﻘﻘﺎن در ﭘﯽ ﭘﺎﺳﺨﮕﻮﯾﯽ ﺑﻪ اﯾﻦ ﭘﺮﺳﺶ ﻫﺴﺘﻨﺪ ﮐﻪ آﯾﺎ ﻣﺼﺮف ﻣﮑﻤﻞ ﮐﻮﺋﺮﺳﺘﯿﻦ، ﻣﯽﺗﻮاﻧﺪ در ﭘﯿﺸﮕﯿﺮي از آﺗﺮوﻓﯽ در دوران ﺑﯽﺗﺤﺮﮐﯽ ﻣﻔﯿﺪ ﺑﺎﺷﺪ؟ در اﯾﻦ ﭘﮋوﻫﺶ، 30 ﺳﺮ ﻣﻮش ﻧﺮ ﺻﺤﺮاﯾﯽ 2 ﻣﺎﻫﻪ ﻧﮋاد اﺳﭙﺮاگداوﻟﯽ (وزن=11±295/80 ﮔﺮم) اﻧﺘﺨﺎب ﺷﺪﻧﺪ و در دو ﮔﺮوه ﮐﻨﺘﺮل (6=n) و ﺗﻤﺮﯾﻦ (24=n) ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺑﺎ ﺷﺪت 16 ﻣﺘﺮ در ﻫﻔﺘﮥ اول آﻏﺎز ﺷﺪ و در ﻫﻔﺘﮥ ﻫﺸﺘﻢ ﺑﻪ ﺳﺮﻋﺖ 30 ﻣﺘﺮ در دﻗﯿﻘﻪ رﺳﯿﺪ. ﭘﺲ از آن، رتﻫﺎي ﮔﺮوه ﮐﻨﺘﺮل و 6 رت از ﮔﺮوه ﺗﻤﺮﯾﻦ ﮐﺸﺘﻪ ﺷﺪﻧﺪ. ﺳﭙﺲ ﻣﺎﺑﻘﯽ ﮔﺮوه ﺗﻤﺮﯾﻦ (18=n) ﺑﻪﺻﻮرت ﺗﺼﺎدﻓﯽ ﺑﻪ ﺳﻪ ﮔﺮوه )ﺑﯽﺗﻤﺮﯾﻨﯽ، ﺑﯽﺗﺤﺮﮐﯽ و ﺑﯽﺗﺤﺮﮐﯽ ﻫﻤﺮاه ﺑﺎ ﻣﺼﺮف ﻣﮑﻤﻞ ﮐﻮﺋﺮﺳﺘﯿﻦ( ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﺳﭙﺲ ﮔﺮوهﻫﺎي ﺑﯽﺗﺤﺮك ﺑﻪ ﻣﺪت 14 روز ﺑﯽﺣﺮﮐﺖ ﺷﺪﻧﺪ و در اﯾﻦ دوران ﺑﻪ ﮔﺮوه ﻣﮑﻤﻞ، ﻣﮑﻤﻞ ﮐﻮﺋﺮﺳﺘﯿﻦ ﺑﺎ دوز 400 ﻣﯿﻠﯽﮔﺮم در روز ﺧﻮراﻧﺪه ﻣﯽﺷﺪ. ﭘﺲ از اﺗﻤﺎم دورة 14 روزه، رتﻫﺎ ﮐﺸﺘﻪ ﺷﺪﻧﺪ و ﻣﯿﺰان ﺑﯿﺎن ژن 1-PGC ﻋﻀﻼت ﻧﻌﻠﯽ آزﻣﻮدﻧﯽﻫﺎ اﻧﺪازهﮔﯿﺮي ﺷﺪ. ﻣﯿﺎﻧﮕﯿﻦ وزﻧﯽ رتﻫﺎ ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل و دو ﮔﺮوه دﯾﮕﺮ (ﺑﯽﺗﻤﺮﯾﻦ و ﺑﯽﺗﺤﺮك) ﺑﯿﺸﺘﺮ ﺑﻮد (0/001 = P). از ﻃﺮف دﯾﮕﺮ، ﭘﺲ از 14 روز، ﻣﯿﺰان ﺑﯿﺎن ژن PGC1α در ﮔﺮوه ﺑﯽﺗﺤﺮك ﻫﻤﺮاه ﺑﺎ ﻣﺼﺮف ﮐﻮﺋﺮﺳﺘﯿﻦ ﺑﯿﺸﺘﺮﯾﻦ ﺳﻄﺢ و در ﮔﺮوه ﺑﯽﺗﺤﺮك ﮐﻤﺘﺮﯾﻦ ﺳﻄﺢ ﺑﯿﺎن ژﻧﯽ را داﺷﺖ (0/004 =P). ﻧﺘﺎﯾﺞ ﺗﻐﯿﯿﺮات ﻧﺸﺎن داد ﺑﺎ ﻣﺼﺮف ﻣﮑﻤﻞ ﮐﻮﺋﺮﺳﺘﯿﻦ، ﻣﯽﺗﻮان اﻧﺘﻈﺎر ﺣﺪاﻗﻠﯽ ﮐﺎﻫﺶ در وزن ﻋﻀﻠﻪ و ﺑﯿﺎن ژﻧﯽPGC1 در ﺑﯽﺗﺤﺮﮐﯽ را داﺷﺖ.

The aim of this study was to determine the effect of Quercetin supplement during sedentary period on the signaling pathway of the PGC-1α. Researchers are trying to find out whether Quercetin supplement consumption can be beneficial to preventing atrophy during sedentary period. In this study, 30 male rats (age=2 months old, weight=295±11.80 g, Sprague-Dawley) were selected. They were assigned to 2 groups: control (n=6) and training (n=24). Interval training with the intensity of 16 m started in the first week and reached the speed of 30 m/min. in the eighth week. Then, the rats of the control group and 6 rats of the training group were killed. Then, the rest of training group (n=18) were randomly divided into 3 groups: detraining, sedentation and sedentation-Quercetin supplement. Sedentary groups became inactive for 14 days and Quercetin supplement group was fed with Quercetin (400 mg/day) during this period. After these 14 days, they were killed and the amount of the PGC-1α gene expression of their soleus muscle was measured. The mean weight of the rats was higher than the control and the other two groups (detraining and sedentation) (P=0.001). On the other hand, after 14 days, the sedentation-Quercetin group had the highest whereas the sedentation group had the lowest amount of PGC-1α gene expression (P=0.004). The results of the changes showed that the minimum reduction in the muscle weight and gene expression of the PGC1α during sedentary period can be expected by consuming Quercetin supplement.