ارزيابي مقاومت آنتي‌بيوتيكي در برابر باكتري ويبريوهاي بيماري‌زا در مراكز تكثير ميگوي سفيد غربي (Litopenaeus vannamei)

Evaluation of endurance against antibiotics in the western blotting shrimp propagation centers on pathogens of vibrio

اﯾﻦ ﺗﺤﻘﯿﻖ ﺑﺎﻫﺪف ارزﯾﺎﺑﯽ ﻣﻘﺎوﻣﺖ در ﺑﺮاﺑﺮ آﻧﺘﯽﺑﯿﻮﺗﯿﮏﻫﺎي ﻣﺮاﮐﺰ ﺗﮑﺜﯿﺮ ﻣﯿﮕﻮي ﺳﻔﯿﺪ ﻏﺮﺑﯽ ﺑﺮ ﺑﺎﮐﺘﺮي وﯾﺒﺮﯾﻮﻫﺎي ﺑﯿﻤﺎريزا اﻧﺠﺎم ﺷﺪ. اﺳﺖ. در اﯾﺮان ﻣﻬﻢﺗﺮﯾﻦ ﺑﺎﮐﺘﺮيﻫﺎي آﺳﯿﺐرﺳﺎن ﺑﻪ ﻣﺰارع ﭘﺮورش ﻣﯿﮕﻮ ﮔﻮﻧﻪﻫﺎي وﯾﺒﺮﯾﻮ اﺳﺖ. در اﺳﺘﺎن ﺑﻮﺷﻬﺮ ﻧﯿﺰ ﺑﻪ ﻋﻠﺖ ﮐﺎراﺋﯽ ﻣﺼﺮف ﺑﺎﻻﯾﯽ ﮐﻪ آﻧﺘﯽﺑﯿﻮﺗﯿﮏﻫﺎي ارﯾﺘﺮوﻣﺎﯾﺴﯿﻦ اﺳﺘﺮﭘﺘﻮﻣﺎﯾﺴﯿﻦ اﮐﺴﯽﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ و ﺗﺮيﻣﯿﺘﻮﭘﺮﯾﻢ در ﺑﯿﻤﺎريﻫﺎي ﺑﺎﮐﺘﺮﯾﺎﯾﯽ دارﻧﺪ ﺑﻪﻋﻨﻮان ﮔﻮﻧﻪﻫﺎي ﻣﻮرد ﺗﺤﻘﯿﻖ اﻧﺘﺨﺎب ﺷﺪﻧﺪ. ﺑﺎﮐﺘﺮيﻫﺎي ﺑﯿﻤﺎريزاي وﯾﺒﺮﯾﻮ ﻫﺎروي و وﯾﺒﺮﯾﻮ آﻟﺠﯿﻨﻮﻟﯿﺘﯿﮑﻮس از ﻣﺮاﮐﺰ ﺗﮑﺜﯿﺮ ﺟﺪا ﺷﺪﻧﺪ و ﺑﺎ اﺳﺘﻔﺎده از روش دﯾﺴﮏ دﯾﻔﯿﻮژن ﺑﻪ ﺗﻌﯿﯿﻦ ﻣﻘﺎوﻣﺖ ﺑﺎﮐﺘﺮيﻫﺎي ﭘﺮداﺧﺘﻪ ﺷﺪ. ﺟﺪاﺳﺎزي ﺑﺎﮐﺘﺮيﻫﺎ و ﺷﻨﺎﺳﺎﯾﯽ ﺑﻪ روش ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ و ﺗﺴﺖﻫﺎي ﺑﯿﻮﻟﻮژﯾﮏ و ﻣﻘﺎﯾﺴﻪ ﻧﺘﺎﯾﺞpH و دﻣﺎﻫﺎي ﻣﺨﺘﻠﻒ ﺑﺮرﺳﯽ ﺷﺪﻧﺪ. ﻫﺮ ﺳﻪ ﺑﺎﮐﺘﺮي وﯾﺒﺮﯾﻮ ﻫﺎروي ﺟﺪاﺳﺎزي ﺷﺪه در ﺳﻪ ﻣﺮﮐﺰ ﺗﮑﺜﯿﺮ A,B,C ﻧﺴﺒﺖ ﺑﻪ آﻧﺘﯽﺑﯿﻮﺗﯿﮏ ارﯾﺘﺮوﻣﺎﯾﺴﯿﻦ ﺑﻪ ﺗﺮﺗﯿﺐ ﻧﯿﻤﻪ ﺣﺴﺎس، ﻣﻘﺎوم و ﺣﺴﺎس ﺑﻮدﻧﺪ. ﻧﺴﺒﺖ ﺑﻪ آﻧﺘﯽﺑﯿﻮﺗﯿﮏ ﺗﺮيﻣﺘﯿﻮﭘﺮﯾﻢ ﻫﺮ ﺳﻪ ﺑﺎﮐﺘﺮي ﺣﺴﺎس ﺑﻮدﻧﺪ. وﯾﺒﺮﯾﻮ آﻟﺠﯿﻨﻮﻟﯿﺘﯿﮑﻮس ﺟﺪاﺳﺎزي ﺷﺪه از ﺳﻪ ﻣﺮﮐﺰ ﺗﮑﺜﯿﺮ ﻧﺴﺒﺖ ﺑﻪ اﺳﺘﺮﭘﺘﻮﻣﺎﯾﺴﯿﻦ ﻣﻘﺎوم ﺑﻮدﻧﺪ. وﻟﯽ ﺑﺎﮐﺘﺮي ﺟﺪاﺳﺎزي ﺷﺪه از ﻣﺮﮐﺰ C ﻧﺴﺒﺖ ﺑﻪ ارﯾﺘﺮوﻣﺎﯾﺴﯿﻦ ﻧﯿﻤﻪ ﺣﺴﺎس و دو ﺑﺎﮐﺘﺮي ﻣﺮﮐﺰ A و B ﻣﻘﺎوم ﺑﻮد. ﻧﺘﺎﯾﺞ ﻧﺸﺎن ﻣﯽدﻫﺪ ﮐﻪ ﺑﯿﺸﺘﺮﯾﻦ ﻣﻘﺎوﻣﺖ آﻧﺘﯽﺑﯿﻮﺗﯿﮑﯽ ﻧﺴﺒﺖ ﺑﻪ اﺳﺘﺮﭘﺘﻮﻣﺎﯾﺴﯿﻦ در ﻣﺮﺣﻠﻪ اول و ﺳﭙﺲ ارﯾﺘﺮوﻣﺎﯾﺴﯿﻦ در ﻣﺮﺣﻠﻪ ﺑﻌﺪ دﯾﺪه ﻣﯽﺷﻮد. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد در pH 6 ﻋﻤﻠﮑﺮد آﻧﺘﯽﺑﯿﻮﺗﯿﮏﻫﺎي ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﭘﺎﯾﯿﻦ ﺑﻮده و و ﺑﺎ اﻓﺰاﯾﺶ pH ﺑﻪ 7 اﯾﻦ ﻣﯿﺰان اﻓﺰاﯾﺶ ﻣﯽﯾﺎﺑﺪ. ﻣﻘﺎوﻣﺖ آﻧﺘﯽﺑﯿﻮﺗﯿﮑﯽ ﺑﺎ درﺟﻪ ﺣﺮارت ﻧﯿﺰ اﺧﺘﻼف ﻣﻌﻨﯽداري را ﻧﺸﺎن داد )0/95

This study was conducted to evaluate the resistance to antibiotics in the western white shrimp propagation centers on pathogens of Vibrio bacteria. In Iran, the most important bacteria in the shrimp are the vibrio species. In Bushehr Province, due to the high efficacy of erythromycin antibiotics, streptomycyne oxytracycline and tremitoprim in bacterial diseases, were selected as the species. The bacterial strains of Vibrio harvey and Vibrio aliginolyticus were isolated from the replication centers and the resistance of the bacteria was investigated using disk diffusion method. Isolation of bacteria and identification by biochemical and biological tests and comparison of pH and different temperatures were investigated. All three Vibrio harvey bacteria isolated from the three replication centers A, B, C, relative to the antibiotic erythromycin, were semi-sensitive, resistant and susceptible, respectively. Three antibiotics were susceptible to trimethoprim for antibiotics. Vibrio oliginolyticus isolated from three proliferation centers was resistant to streptomycin. But the isolated bacteria from center C were resistant to semi-sensitive erythromycin and the two bacteria A and B. The results showed that the highest antibiotic resistance to streptomycin at the first stage and then erythromycin is seen at the next stage. The results showed that the pH of the antibiotics was low at pH 6 and increased to 7 by increasing pH. Antibiotic resistance with temperature is also significant (P<0.95). The analysis showed that temperatures of 35 oC are the most appropriate temperature for Vibrio bacteria. Assessment in aquaculture also has many challenges. In particular, this assessment of antibiotics is one of the key elements in the health of the community.