پديد آورندگان :
رسولي، زهرا داﻧﺸﮕﺎه ﻓﺮدوﺳﯽ ﻣﺸﻬﺪ - داﻧﺸﮑﺪه ﮐﺸﺎورزي - ﮔﺮوه زراﻋﺖ , پارسا, مهدي داﻧﺸﮕﺎه ﻓﺮدوﺳﯽ ﻣﺸﻬﺪ - داﻧﺸﮑﺪه ﮐﺸﺎورزي - ﮔﺮوه زراﻋﺖ
كليدواژه :
اﺳﭙﯿﺮوﻟﯿﻨﺎ , اﺗﻮﺗﺮوف , ﺷﻮري و ﮔﻠﻮﮐﺰ , ﻣﯿﮑﺴﻮﺗﺮوف
چكيده فارسي :
ﺷﺪت ﺗﻐﯿﯿﺮات درون ﺳﻠﻮل، ﺑﺴﺘﻪ ﺑﻪ ﻣﺪت و ﺷﺪت ﺗﻨﺶ ﻣﺘﻔﺎوت اﺳﺖ، ﻟﯿﮑﻦ اﻣﮑﺎن ﺑﻬﺮهوري از ﺗﻨﺶﻫﺎ در اﺑﻌﺎد ﺧﺎص، ﺟﻬﺖ اﻓﺰاﯾﺶ ﺗﻮﻟﯿﺪ ﺗﺮﮐﯿﺒﺎت ﻫﺪف وﺟﻮد دارد. در اﯾﻦ ﻣﯿﺎن اﮔﺮ ﺷﺮاﯾﻄﯽ ﺑﺮاي اﻓﺰاﯾﺶ ﻋﻤﻠﮑﺮد ﮐﻞ ﻓﺮاﻫﻢ آﯾﺪ، ﻣﯽﺗﻮان ﺑﺮ ﻋﻤﻠﮑﺮد ﻣﻮاد ﻣﺆﺛﺮه ﻧﯿﺰ اﻓﺰود. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، ﺗﺄﺛﯿﺮ ﺳﻄﻮح ﻣﺨﺘﻠﻒ ﻧﻤﮏ و ﮐﺮﺑﻮﻫﯿﺪرات ﺟﻬﺖ ﺑﺮرﺳﯽ ﮔﻮﻧﻪ زراﻋﯽ ﻧﻮﯾﻦ Spirulina platensis در ﻣﺤﯿﻂﻫﺎي اﺗﻮﺗﺮوﻓﯿﮏ و ﻣﯿﮑﺴﻮﺗﺮوﻓﯿﮏ ﺑﻪﻣﻨﻈﻮر اﻓﺰاﯾﺶ ﺗﻮﻟﯿﺪ رﻧﮕﺪاﻧﻪﻫﺎ ﻃﯽ ﺳﺎلﻫﺎي 1393-95 اﻧﺪازهﮔﯿﺮي ﺷﺪﻧﺪ. ﺳﻄﻮح ﺑﻪﮐﺎررﻓﺘﻪ ﺷﺎﻣﻞ 1/5 ،1 و 3 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ ﻧﻤﮏ ﺳﺪﯾﻢ ﮐﻠﺮﯾﺪ و 0/5 ،0 و 6 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ ﮔﻠﻮﮐﺰ در ﻗﺎﻟﺐ آزﻣﺎﯾﺶ ﻓﺎﮐﺘﻮرﯾﻞ ﺑﺎ ﻃﺮح ﮐﺎﻣﻼ ً ﺗﺼﺎدﻓﯽ ﺑﻮدﻧﺪ. ﺑﺮ اﺳﺎس ﻧﺘﺎﯾﺞ، ﺗﯿﻤﺎر ﻣﯿﮑﺴﻮﺗﺮوﻓﯽ داراي 1 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ ﻧﻤﮏ و 0/5 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ ﮔﻠﻮﮐﺰ ﺟﻬﺖ اﻓﺰاﯾﺶ ﻣﻘﺪار ﺑﯿﻮﻣﺲ )108/6 درﺻﺪ، 11/97 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ وزنﺗﺮ و 1/56 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ وزن ﺧﺸﮏ(، ﮐﻠﺮوﻓﯿﻞ )136/0 درﺻﺪ، 0/34 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ(، ﮐﺎروﺗﻨﻮﺋﯿﺪﻫﺎ )137/5 درﺻﺪ، 0/11 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ(، ﻓﻼوﻧﻮﺋﯿﺪ )0/09 ،%103/5 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ( و ﺗﯿﻤﺎر ﻣﯿﮑﺴﻮﺗﺮوﻓﯽ 1/5 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ ﻧﻤﮏ و 0/5 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ ﮔﻠﻮﮐﺰ ﺑﺮاي ارﺗﻘﺎي ﻣﻘﺪار آﻧﺘﻮﺳﯿﺎﻧﯿﻦ )0/04 ،111%/8 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ(، آﻟﻮﻓﯿﮑﻮﺳﯿﺎﻧﯿﻦ )0/47 ،%134/3 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ( ﻧﺴﺒﺖ ﺑﻪ ﺗﯿﻤﺎر ﺷﺎﻫﺪ اﺗﻮﺗﺮوف، ﻓﯿﮑﻮﺑﯿﻠﯽﭘﺮوﺗﺌﯿﻦﻫﺎ )1/09 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ(، ﻓﯿﮑﻮﺳﯿﺎﻧﯿﻦ )0/44 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ( و ﻓﯿﮑﻮارﯾﺘﺮﯾﻦ )0/47 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ( ﻣﻌﺮﻓﯽ ﻣﯽﺷﻮﻧﺪ. اﯾﻦ ﻣﯿﺰان ﻣﻮاد ﻣﺆﺛﺮه اﺳﺎﺳﯽ ﺟﻠﺒﮏ در دو ﺗﯿﻤﺎر ﭘﯿﺸﻨﻬﺎدﺷﺪه، اﻣﮑﺎن ﮐﺎرﺑﺮد آن در ﺻﻨﺎﯾﻊ ﻏﺬاﯾﯽ )رﻧﮓ، اﺳﺎﻧﺲ، آرد، ﻏﺬاي دام( و داروﯾﯽ )داروﻫﺎي ﻣﮑﻤﻞ ﻏﺬاﯾﯽ و درﻣﺎﻧﯽ( را ﺑﯿﺎن ﻣﯽﻧﻤﺎﯾﺪ
چكيده لاتين :
Although intensity of intracellular changes varies depending on the duration and intensity of the stress, the possibility of optimum productivity under salinity stress in certain dimensions is likely to increase the percentage of the desired compounds. In this case, if the conditions are provided to increase the total yield, then the compounds yields can be also enhanced. In this research, which has done during 2014-16, we experimentally investigate the effects of salt and carbohydrate levels on Spirulina platensis pigments in autotrophic and heterotrophic conditions. The levels used were 1, 1.5 and 3 g l-1 NaCl and 0, 0.5 and 6 g l-1 glucose in a completely randomized factorial experiment. Based on the results, mixotrophic culture containing 1 g l-1 NaCl and 0.5 g l-1 glucose introduced to increase the amount of biomass (108.6%, 11.97 g l-1 fresh weight and 1.56 g l-1 dry weight), Chlorophyll (136.0%, 0.34 g l-1), carotenoids (137.5%, 0.11 g l-1), flavonoid (103.5%, 0.09 g l-1); and mixotrophic culture of 1/5 g l-1 NaCl and 0.5 g l-1 glucose presented to increase the anthocyanin (111.8%, 0.04 g l-1) and allophycocyanin (134.3%, 0.47 g l-1) contents compared to autotrophic control treatment, phycobilioproteins (1.09 g l-1), phycocyanin (0.44 g l-1) and phycoerythrin (0.47 g l-1). This amount of different active substances of algae in the proposed treatments can be used in food (color, essence, flour, livestock feed) and pharmaceutical (supplementary food and medicine) industries.
