توليد پروتئين هيدروليز شده از ضايعات ماهي زالون (Alosa caspia) با استفاده از آنزيم آلكالاز

Production of protein hydrolysates from Caspian shad (Alosa caspia) by-products using Alcalase enzyme

در ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ ﭘﺮوﺗﺌﯿﻦ ﻫﯿﺪروﻟﯿﺰ ﺷﺪه از ﺿﺎﯾﻌﺎت )ﺳﺮ، ﭘﻮﺳﺖ، اﻣﻌﺎء و اﺣﺸﺎء( ﻣﺎﻫﯽ زاﻟﻮن ) Alosa caspia( ﮔﻮﻧﻪ Clupeonella در درﯾﺎي ﺧﺰر ﺗﻮﻟﯿﺪ ﺷﺪ )1394(. ﭘﺮوﺗﺌﯿﻦ ﻫﯿﺪروﻟﯿﺰ در ﺳﻪ زﻣﺎن ﻣﺨﺘﻠﻒ )15، 30 و 60 دﻗﯿﻘﻪ(، ﺑﺎ اﺳﺘﻔﺎده از آﻧﺰﯾﻢ ﺗﺠﺎري آﻟﮑﺎﻻز در pH) 8/5 pH ﺑﻬﯿﻨﻪ ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢ آﻟﮑﺎﻻز( و ﺑﺎ ﻧﺴﺒﺖ آﻧﺰﯾﻢ ﺑﻪ ﺳﻮﺑﺴﺘﺮاي ﻧﻤﻮﻧﻪ اوﻟﯿﻪ )1 ﺑﻪ 100(، در درﺟﻪ ﺣﺮارت 50 درﺟﻪ ﺳﺎﻧﺘﯽ ﮔﺮاد ﺗﻮﻟﯿﺪ ﺷﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﭘﺮوﺗﺌﯿﻦ ﻫﯿﺪروﻟﯿﺰ ﺷﺪه در زﻣﺎن 60 دﻗﯿﻘﻪ ﺑﻪﺻﻮرت ﻣﻌﻨﯽداري ازﻟﺤﺎظ ﻣﯿﺰان ﺑﺎزﯾﺎﻓﺖ ﭘﺮوﺗﺌﯿﻨﯽ و درﺟﻪ ﻫﯿﺪروﻟﯿﺰاﺳﯿﻮن ﺑﺎﻻﺗﺮ از ﺳﺎﯾﺮ ﭘﺮوﺗﺌﯿﻦﻫﺎي ﻫﯿﺪروﻟﯿﺰ ﺷﺪه ﺑﻮد )0/05

Protein hydrolysates were produced from by-products (head, skin and viscera) of Caspian shad (Alosa caspia), a major Clupeonella species in the Caspian Sea (2016). Hydrolysis was performed at three different times (15, 30 and 60 min), pH 8.5, using commercial enzyme Alcalase and an enzyme to substrate ratio of (1 to 100) by-product at 50°C. The results indicated that, Protein recovery and degree of hydrolysis increased with increasing incubation time (60 min) (P<0.05). Protein and lipid content of the hydrolysate were 78.91%, and 0.09%, respectively. The chemical score results showed that hydrolysates could fulfill human amino acid requirements. The protein efficiency ratio showed that hydrolysates had high nutritional value. According to high nutritional values, this can be used as protein source in fish feed.