كليدواژه :
آﻧﺰﯾﻢﻫﺎي آﻧﺘﯽ اﮐﺴﯿﺪان , ﭘﺮوﺗﺌﯿﻦ ﻣﺤﻠﻮل ﮐﻞ , ﻧﺎﻧﻮ ﻓﻨﺎوري , ﻧﺎﻧﻮ ذرات ﻧﻘﺮه
چكيده فارسي :
در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺎﺛﯿﺮ ﻧﺎﻧﻮ ذرات ﻧﻘﺮه در اﻧﺪازهﻫﺎي ﺑﯿﻦ 53/94 و 288/5 ﻧﺎﻧﻮﻣﺘﺮ و در ﻏﻠﻈﺖﻫﺎي 20 ،10 ،5 ،2/5 ،0،
80 ،40 و 100 ﭘﯽﭘﯽام ﺑﺮ روي ﺗﻐﯿﯿﺮات ﺷﺎﺧﺺ ﻫﺎي ﻓﯿﺰﯾﻮﯾﻮﻟﻮژﯾﮑﯽ ﻣﺎﻧﻨﺪ آﻧﺘﻮﺳﯿﺎﻧﯿﻦ ﮐﻞ، اﻟﮕﻮي ﭘﺮوﺗﺌﯿﻦ ﻣﺤﻠﻮل ﮐﻞ و ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢﻫﺎي ﮐﺎﺗﺎﻻز، آﺳﮑﻮرﺑﺎت ﭘﺮاﮐﺴﯿﺪاز و ﺳﻮﭘﺮاﮐﺴﯿﺪ دﯾﺴﻤﻮﺗﺎز ﮔﯿﺎﻫﭽﻪﻫﺎي ﮔﻮﺟﻪ )Solanum Lycopersicon ( در ﻣﺤﯿﻂ ﮐﺸﺖ درون ﺷﯿﺸﻪ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮارﮔﺮﻓﺖ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﻣﯿﺰان آﻧﺘﻮﺳﯿﺎﻧﯿﻦ در ﭘﺎﺳﺦ ﺑﻪ ﺣﻀﻮر ﻧﺎﻧﻮذرات ﻧﻘﺮه ﮐﺎﻫﺶ ﻣﻌﻨﯽداري را ﺗﺎ ﻏﻠﻈﺖ 10 ﭘﯽﭘﯽام ﺑﻪ دﻧﺒﺎل داﺷﺘﻪ اﺳﺖ. ﭘﺮوﺗﺌﯿﻦ ﻣﺤﻠﻮل ﮐﻞ ﮔﯿﺎه ﺑﺎ اﻓﺰاﯾﺶ ﻏﻠﻈﺖ ﻧﺎﻧﻮذره در اﻧﺪام ﻫﻮاﯾﯽ اﻓﺰاﯾﺶ و در رﯾﺸﻪ ﺑﺎ اﻓﺰاﯾﺶ ﻏﻠﻈﺖ در 20 و 40 ﭘﯽﭘﯽام ﻧﺴﺒﺖ ﺑﻪ ﺷﺎﻫﺪ ﮐﺎﻫﺶ ﻧﺸﺎن داد. ﻫﻤﭽﻨﯿﻦ ﺷﺪت ﺑﯿﺎن ﺷﺶ ﺑﺎﻧﺪ ﭘﺮوﺗﺌﯿﻨﯽ ﺗﺤﺖ ﺗﯿﻤﺎر ﺑﺎ ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﺗﻐﯿﯿﺮ ﻧﻤﻮد. ﺑﺎ اﻓﺰاﯾﺶ ﻏﻠﻈﺖ ﻧﺎﻧﻮ ذرات در ﻣﺤﯿﻂ ﮐﺸﺖ اﻓﺰاﯾﺶ در ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢﻫﺎي آﻧﺘﯽ اﮐﺴﯿﺪاﻧﺖ در رﯾﺸﻪﻫﺎ و اﻧﺪام ﻫﻮاﯾﯽ ﻣﺸﺎﻫﺪه ﮔﺮدﯾﺪ.
چكيده لاتين :
TodayToday, synthetic nanoparticles encompass a wide range of particles with unique properties and have many applications in the field of nanotechnology. Due to the emergence and lack of understanding of the consequences of using nanoparticles, investigating the effect of releasing these particles in the environment at various biological levels is important. In this study, the effects of nanoparticles of silver in concentrations of 0, 2.5, 5, 10, 20, 40, 80 and 100 ppm on physiological parameters such as total anthocyanin, total soluble protein and enzyme activities of Catalase, Ascorbate peroxidase and Superoxide dismutase in tomato seedlings (Solanum Lycopersicon) under in vitro were evaluated. The results showed that anthocyanin levels in response to the presence of silver nanoparticles decreased significantly to 10 ppm concentration. The total protein soluble protein increased with increase in the concentration of nanoparticles in the shoot and decreased with increasing concentrations at 20 and 40 ppm compared to the control. The intensity of expression of six protein bands treated with silver nanoparticles also changed. Increasing the concentration of nanoparticles in the medium increased the activities of catalase and superoxide dismutase and decreased ascorbate peroxidase activity in the roots. However, in shoot, increased activity of peroxidase and catalase, and decreased activity of superoxide dismutase in response to high concentrations of silver nanoparticles. The results indicated that the presence of silver nanoparticles in the tomato culture medium leads to responses at physiological and molecular levels.
