اثر زيره سياه بر فاكتورهاي آنتي اكسيداني و پارامتر‌هاي استرس اكسيداتيو در رت‌هاي مواجه شده با 1 و 2- دي متيل هيدرازين

Effects of Carum carvi L. on Antioxidant status and oxidative stress parameters in rats exposed to 1, 2-dimethyl hydrazine

در اﯾﻦ ﺗﺤﻘﯿﻖ، ﺗﺎﺛﯿﺮﮐﺎرﺑﺮد ﭘﻮدر و اﺳﺎﻧﺲ زﯾﺮه ﺳﯿﺎه ﺑﺎ دوز 0/2 درﺻﺪ )w/w( در ﻣﻬﺎر اﯾﺠﺎد ﺳﺮﻃﺎن ﮐﻮﻟﻮن اﻟﻘﺎء ﺷﺪه ﺑﺎ دي ﻣﺘﯿﻞ ﻫﯿﺪرازﯾﻦ )DMH( ﺑﺮ روي ﺗﻐﯿﯿﺮات ﺷﺎﺧﺺﻫﺎي ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ ﻣﺎﻧﻨﺪ آﻧﺰﯾﻢﻫﺎي ﮐﺎﺗﺎﻻز )CAT( ، ﺳﻮﭘﺮاﮐﺴﯿﺪ دﯾﺴﻤﻮﺗﺎز )SOD( ، ﮔﻠﻮﺗﺎﺗﯿﻮن اﺣﯿﺎ )GSH( ، ﭘﺮاﮐﺴﯿﺪاﺳﯿﻮن ﻟﯿﭙﯿﺪ )LP( و ﻇﺮﻓﯿﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﮐﻞ ﭘﻼﺳﻤﺎ )FRAP( و ﻫﻤﭽﻨﯿﻦ ﺗﻐﯿﯿﺮات ﭘﺎﺗﻮﻟﻮژﯾﮑﯽ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺗﺰرﯾﻖ DMH ﺑﺎﻋﺚ اﻓﺰاﯾﺶ GSH و FRAP و ﮐﺎﻫﺶ SOD ﮔﺮدﯾﺪ. در ﺣﺎﻟﯿﮑﻪ ﺗﯿﻤﺎر رتﻫﺎ ﺑﺎ اﺳﺎﻧﺲ و ﭘﻮدر زﯾﺮه ﺳﯿﺎه ﺗﺎﺛﯿﺮي ﺑﺮ روي ﺑﻬﺒﻮد ﭘﺎراﻣﺘﺮﻫﺎي ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ ﻧﺪاﺷﺘﻪ اﺳﺖ، ﻧﺘﺎﯾﺞ ﭘﺎﺗﻮﻟﻮژي ﺗﺎﺛﯿﺮ ﻣﺜﺒﺖ ﺗﯿﻤﺎرﻫﺎ را در روﻧﺪ ﺳﺮﻃﺎﻧﺰاﯾﯽ ﻧﺸﺎن دادﻧﺪ. ﻟﺬا ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﻧﺘﺎﯾﺞ ﺑﻪ دﺳﺖ آﻣﺪه، ﻓﻌﺎﻟﯿﺖ ﺿﺪ ﺳﺮﻃﺎﻧﯽ اﺳﺎﻧﺲ و ﭘﻮدر زﯾﺮه ﺳﯿﺎه، اﺣﺘﻤﺎﻻ ﻣﺮﺑﻮط ﺑﻪ اﺛﺮات آن در ﻣﺴﯿﺮﻫﺎي دﯾﮕﺮ ﻣﺘﺎﺑﻮﻟﯿﮑﯽ )ﻏﯿﺮ از ﺳﯿﺴﺘﻢ دﻓﺎع آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ و اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ( ﻣﯽ باشد.

In the current study, the effect of usage of caraway powder and its essential oils at the dose of 0.2% (w/w) were studied on colon cancer prevention induced by dimethyl hydrazine (DMH) through the estimation of biochemical parameters including catalase (CAT) and superoxide dismutase (SOD) enzymes, glutathione (GSH), lipid peroxidation (LP) and ferric reducing ability of plasma (FRAP) as well as histopathological changes. The results showed that the levels of GSH and FRAP were increased and SOD activity was decreased by DMH. Even though caraway powder and its essential oils failed to alter the tested biochemical parameters, histopathological analysis indicated improved effects in carcinogenesis. Therefore, the results demonstrated that the chemopreventive effects of the treatments were due to the other metabolic pathways than oxidative stress/antioxidant systems.