اثر تمرينات هوازي تداومي (cat) و تناوبي شديد(hiit) بر بيان ژن «پروتئين داراي منطقۀ تنظيمي مثبت 16» (prdm16) در بافت چربي سفيد موش‌هاي صحرايي ويستار

The Effect of Continuous Aerobic Training (CAT) and High Intensity Interval Training (HIIT) on Gene Expression of Positive Regulatory Domain-Containing Protein 16 (PRDM16) in White Adipose Tissue of Wistar Rats

ﻣﻄﺎﻟﻌﺎت ﮔﺬﺷﺘﻪ، اﺛﺮ ﺗﻤﺮﯾﻦ ﻫﻮازي ﺗﺪاوﻣﯽ )CAT( را ﺑﺮ ﺑﯿﺎن ژن »ﭘﺮوﺗﺌﯿﻦ داراي ﻣﻨﻄﻘﮥ ﺗﻨﻈﯿﻤﯽ ﻣﺜﺒﺖ 16« )PRDM16( در ﺑﺎﻓﺖ ﭼﺮﺑﯽ ﺳﻔﯿﺪ ﺑﺮرﺳﯽ ﮐﺮدهاﻧﺪ. ﺑﺎ اﯾﻦ ﺣﺎل، ﺗﺎﮐﻨﻮن ﻣﻄﺎﻟﻌﻪاي اﺛﺮ اﻧﻮاع ﻣﺪلﻫﺎي ﺗﻤﺮﯾﻨﯽ ﺑﻪوﯾﮋه ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ )HIIT( را ﺑﺮ ﺑﯿﺎن اﯾﻦ ژن ﻣﻄﺎﻟﻌﻪ ﻧﮑﺮده اﺳﺖ. ﺑﻨﺎﺑﺮاﯾﻦ، در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، اﺛﺮ دو ﺷﯿﻮة ﺗﻤﺮﯾﻦ ﻫﻮازي ﺗﺪاوﻣﯽ )CAT( و ﻫﻤﭽﻨﯿﻦ دو ﺷﯿﻮة ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ )HIIT( ﺑﺮ ﺑﯿﺎن اﯾﻦ ژن ﺑﺮرﺳﯽ ﺷﺪ. 40 ﺳَﺮ رَت ﺑﻪﺻﻮرت ﺗﺼﺎدﻓﯽ ﺑﻪ ﭘﻨﺞ ﮔﺮوه: ﮐﻨﺘﺮل، ﺗﻤﺮﯾﻦ ﻫﻮازي ﺑﺎ ﺣﺠﻢ ﻣﺘﻮﺳﻂ، ﺗﻤﺮﯾﻦ ﻫﻮازي ﺑﺎ ﺣﺠﻢ زﯾﺎد، ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﺑﺎ ﺣﺠﻢ ﻣﺘﻮﺳﻂ و ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﺑﺎ ﺣﺠﻢ زﯾﺎد ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. آزﻣﻮدﻧﯽﻫﺎي ﮔﺮوهﻫﺎي ﺗﻤﺮﯾﻨﯽ، ﺑﻪ ﻣﺪت 8 ﻫﻔﺘﻪ، ﻫﻔﺘﻪاي 5 ﺟﻠﺴﻪ، ﺗﺤﺖ دو ﺷﯿﻮة ﺗﻤﺮﯾﻦ ﺗﻤﺮﯾﻦ ﻫﻮازي ﺗﺪاوﻣﯽ و دو ﺷﯿﻮة ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﺑﺮ روي ﻧﻮار ﮔﺮدان ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﭘﺲ از اﺗﻤﺎم ﭘﺮوﺗﮑﻞ ﺗﻤﺮﯾﻦ، آزﻣﻮدﻧﯽﻫﺎ ﺑﯽﻫﻮش ﺷﺪه و ﺑﺎﻓﺖ ﭼﺮﺑﯽ زﯾﺮﭘﻮﺳﺘﯽ ﺑﺮداﺷﺘﻪ ﺷﺪﻧﺪ. ﺑﺮاي اﻧﺪازهﮔﯿﺮي ﺑﯿﺎن ﻧﺴﺒﯽ ژنِ PRDM16 از روش Real Time –PCR اﺳﺘﻔﺎده ﺷﺪ. دادهﻫﺎ ﻧﺸﺎن داد ﻣﯿﺰان ﺑﯿﺎن ژن PRDM16 در دو ﮔﺮوه »ﺗﻤﺮﯾﻦ ﻫﻮازي ﺗﺪاوﻣﯽ ﺑﺎ ﺣﺠﻢ ﻣﺘﻮﺳﻂ« و »ﺑﺎ ﺣﺠﻢ زﯾﺎد« و در دو ﮔﺮوه »ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﺑﺎ ﺣﺠﻢ ﻣﺘﻮﺳﻂ« و »ﺣﺠﻢ ﻧﺴﺒﺘﺎً زﯾﺎد«، ﺗﻔﺎوت ﻣﻌﻨﺎداري در ﻗﯿﺎس ﺑﺎ ﮔﺮوه ﮐﻨﺘﺮل ﻧﺪاﺷﺖ )0/05>P(. ﯾﺎﻓﺘﻪﻫﺎي اﯾﻦ ﻣﻄﺎﻟﻌﻪ دﻻﻟﺖ ﺑﺮ اﯾﻦ دارﻧﺪ ﮐﻪ ﺗﻤﺮﯾﻨﺎت ورزﺷﯽ اﻋﻢ از ﺗﻤﺮﯾﻦ ﻫﻮازي ﺗﺪاوﻣﯽ و ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ، ﺗﻐﯿﯿﺮي در ﺑﯿﺎن ژن PRDM16 در ﺑﺎﻓﺖ ﭼﺮﺑﯽ زﯾﺮﭘﻮﺳﺘﯽ اﯾﺠﺎد ﻧﻤﯽﮐﻨﺪ.

Previous studies have investigated the effect of continuous aerobic training (CAT) on gene expression of positive regulatory domain-containing protein 16 (PRDM16) in white adipose tissue. However, no study has yet investigated the effect of different models of training especially high intensity interval training (HIIT) on the expression of this gene. Thus, the aim of this study was to investigate the effect of two models of HIIT and two models of CAT on gene expression of PRDM16. 40 rats were divided randomly into five groups: 1) control 2) moderate volume CAT 3) high volume CAT 4) moderate volume HIIT and 5) high volume HIIT. The subjects of training groups underwent two models of HIIT and two models of CAT on a treadmill for 8 weeks, 5 sessions per week. After the last training session, rats were euthanized and subcutaneous adipose tissue were dissected. The Real Time–PCR method was used to measure the relative gene expression of PRDM16. Data showed that the gene expression of PRDM16 had no significant difference in two HIIT and two CAT groups compared with the control group (P>0.05). The results of this study indicated that training exercises including CAT and HIIT do not change the gene expression of PRDM16 in subcutaneous adipose tissue.