اثر مصرف مكمل امگا-3 بر پروتئين هاي فاز حاد زنان ورزشكار پس از يك وهله فعاليت ورزشي درمانده‌ساز

The effect of omega-3 supplementation on acute phase proeins in athletic women after single bout of exhaustive exercise

هدف ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﺑﺮرﺳﯽ اﺛﺮ ﻣﺼﺮف ﻣﮑﻤﻞ اﻣﮕﺎ-3 ﺑﺮ ﭘﺮوﺗﺌﯿﻦ ﻓﺎز ﺣﺎد )CRP( و ﻓﯿﺒﺮﯾﻨﻮژن زﻧﺎن ورزﺷﮑﺎر ﭘﺲ از اﻧﺠﺎم ﯾﮏ وﻫﻠﻪ ﻓﻌﺎﻟﯿﺖ ورزﺷﯽ درﻣﺎﻧﺪهﺳﺎز ﺑﻮد. در اﯾﻦ ﭘﮋوﻫﺶ 14داﻧﺸﺠﻮي دﺧﺘﺮ ورزﺷﮑﺎر ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ و اﻧﺤﺮاف اﺳﺘﺎﻧﺪارد ﺳﻨﯽ، ﺗﻮدة ﺑﺪن و ﺣﺪاﮐﺜﺮ اﮐﺴﯿﮋن ﻣﺼﺮﻓﯽ ﺑﻪﺗﺮﺗﯿﺐ 21/17±0/78 ﺳﺎل، 21/21±2/32 ﮐﯿﻠﻮﮔﺮم ﺑﺮ ﻣﺘﺮ ﻣﺮﺑﻊ و 45/74±4/97 ﻣﯿﻠﯽﻟﯿﺘﺮ ﮐﯿﻠﻮﮔﺮم ﺑﺮ دﻗﯿﻘﻪ ﺑﻪﺻﻮرت داوﻃﻠﺒﺎﻧﻪ در اﯾﻦ ﭘﮋوﻫﺶ ﺷﺮﮐﺖ ﮐﺮدﻧﺪ و ﺑﻪﻃﻮر ﺗﺼﺎدﻓﯽ ﺑﻪ دو ﮔﺮوه داروﻧﻤﺎ و ﻣﮑﻤﻞ ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﮔﺮوه ﻣﮑﻤﻞ روزاﻧﻪ ﯾﮏ ﻋﺪد ﮐﭙﺴﻮل اﻣﮕﺎ-3 ﺣﺎوي 3/2 ﮔﺮم اﯾﮑﻮﺳﺎﭘﻨﺘﺎﻧﻮﯾﯿﮏ اﺳﯿﺪ )EPA( و 2/2 ﮔﺮم دوﮐﻮﺳﺎﻫﮕﺰاﻧﻮﯾﯿﮏ اﺳﯿﺪ )DHA( و ﮔﺮوه داروﻧﻤﺎ ﮐﭙﺴﻮل داروﻧﻤﺎ را در ﻃﻮل 3 ﻫﻔﺘﻪ ﻣﺼﺮف ﮐﺮدﻧﺪ. ﻧﻤﻮﻧﻪﻫﺎي ﺧﻮﻧﯽ ﺑﻪﻣﻨﻈﻮر ﺳﻨﺠﺶ CRP و ﻓﯿﺒﺮﯾﻨﻮژن در اﺑﺘﺪا و ﭘﺎﯾﺎن دورة ﻣﮑﻤﻞﺳﺎزي ﭘﯿﺶ و ﭘﺲ از ﻓﻌﺎﻟﯿﺖ ورزﺷﯽ درﻣﺎﻧﺪهﺳﺎز و ﺑﻪﺻﻮرت ﻧﺎﺷﺘﺎ ﺟﻤﻊآوري ﺷﺪ. ﻓﻌﺎﻟﯿﺖ ورزﺷﯽ درﻣﺎﻧﺪهﺳﺎز ﺷﺎﻣﻞ دوﯾﺪن ﺑﺮ روي ﻧﻮار ﮔﺮدان و اﻓﺰاﯾﺶ ﺷﯿﺐ ﺗﺎ رﺳﯿﺪن ﺑﻪ ﺣﺪ واﻣﺎﻧﺪﮔﯽ ﺑﻮد. ﺑﻪﻣﻨﻈﻮر ﺗﺠﺰﯾﻪوﺗﺤﻠﯿﻞ آﻣﺎري اﻃﻼﻋﺎت ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮن ﮐﻮﻟﻤﻮﮔﺮوف-اﺳﻤﯿﺮﻧﻮف ﻃﺒﯿﻌﯽ ﺑﻮدن ﺗﻮزﯾﻊ دادهﻫﺎ ﺑﺮرﺳﯽ ﺷﺪ، ﺳﭙﺲ دادهﻫﺎ ﺑﻪوﺳﯿﻠﮥ آﻧﺎﻟﯿﺰ وارﯾﺎﻧﺲ دوراﻫﻪ در ﺳﻄﺢ ﻣﻌﻨﺎداري 0/05

The aim of this study was to examine the effect of omega-3 supplementation on C-reactive protein (CRP) and fibrinogen in female athletes after a single bout of exhaustive exercise. 14 female athlete students (mean ± SD age 21.17±0.78 years, body mass 21.21±2.32 kg/m2, VO2max 45.74±4.97 ml/kg/m) voluntarily participated in this study. They were randomly divided into two groups: placebo and supplement. The supplement group daily received one capsule of omega-3 containing 3.2 g of eicosapentaenoic acid (EPA) and 2.2 g of docosahexaenoic acid (DHA) while the placebo group consumed placebo capsules for 3 weeks. Fasting blood samples were collected at the beginning and at the end of the supplementation period, before and after the exhaustive exercise to measure CRP and fibrinogen. The exhaustive exercise included running on a treadmill and an increase in the slope until exhaustion. For data analysis, Kolmogorov-Smirnov test was used to check the normal distribution of data. Then, the data were analyzed by two-way ANOVA. Statistical differences were considered significant at P<0.05. The findings showed that inflammatory factors of CRP and fibrinogen significantly increased after the exercise (P=0.001). 3 weeks of omega-3 supplementation decreased serum CRP after a single bout of exercise but this effect was not significant (P=0.54). In the supplement group, omega-3 supplementation significantly decreased fibrinogen after the exercise (P=0.005). It seems that this supplement or nutrients full of omega-3 fatty acids such as fish are useful for decreasing inflammation due to the exercises.