سابقه و هدف: عملكرد توليدمثلي نژادهاي مختلف گوسفند در ايران پايين است و پرورش اين گونه دامي از نظر اقتصادي زياد به صرفه نيست. بنابراين بايد راهكارهاي اساسي و كاربردي براي افزايش عملكرد توليدمثلي در گوسفند انجام شود. يكي از راهكارهاي مناسب استفاده از برنامههاي مختلف هورمون درماني نظير استفاده از گونادوتروپين جفت انساني و هورمون آزادسازي گونادوتروپينها در ميشهاي سوپراوولاسيونشده (تخمكريزي چندتايي) با گونادوتروپين جفت اسبسانان، است. با اين وجود، نتايج مطالعات گوناگون در ارتباط با كاربرد اين هورمونها در نژادهاي مختلف، متفاوت است. لذا مطالعه حاضر براي بررسي تاثير اين هورمونها بر عملكرد توليدمثلي گوسفندان لك و تركي قشقايي در شرايط پرورش عشايري انجام شد.
مواد و روشها: پژوهش حاضر بهصورت دو آزمايش مجزا انجام شد و در هر آزمايش 75 راس ميش لك و تركي قشقايي (3 تا 4 ساله) و به-ترتيب با ميانگين وزني 6/254 و 9/159 كيلوگرم انتخاب شدند. چرخه فحلي ميشها در فصل توليدمثلي با استفاده از اسفنجهاي محتوي پروژسترون براي يك دوره 14 روزه همزمان شد. آنگاه يك روز قبل از خارج نمودن اسفنجها، به ميشهاي لك و تركي قشقايي بهترتيب 500 و 600 واحد بينالمللي گونادوتروپين جفت اسبسانان به شكل درون ماهيچهاي تزريق شد. بعد از برداشت اسفنج، همه ميشها فحل شده و به سه گروه مختلف تيماري تقسيم شدند. گروه اول در هر آزمايش بهعنوان شاهد در نظر گرفته شد و به گروههاي دوم و سوم در هر آزمايش بهترتيب 500 واحد بينالمللي گونادوتروپين جفت انساني و نيم ميليليتر هورمون آزادسازي گونادوتروپينها تزريق شد. سپس در هر آزمايش فراسنجههايي نظير زمان شروع فحلي(ساعت)، نرخ بازگشت به فحلي، نرخ آبستني، نرخ زايش، نرخ چند قلوزايي، تعداد برههاي متولد شده و نرخ برهزايي اندازهگيري شد.
يافتهها: نتايج در ميشهاي لك قشقايي (آزمايش اول) نشان داد كه نرخ بازگشت به فحلي بين گروههاي مختلف تيماري تفاوت معنيداري نداشت (05/0
چكيده لاتين :
Background and objectives: Reproductive performance of different sheep races is low in Iran and breeding of this domestic animal is not really economic. So it should be found essential and practical solutions for increasing reproductive performance of sheep. One of the suitable solutions is hormonetrapy such as using human Chorionic Gonadotropine (hCG) and Gonadotropin Releasing Hormone (GnRH) in superovulated ewes with equine Chorionic Gonadotropin (eCG), but, the results of different studies in hormonetrapy if different races are not similar. Therefore the present study was conducted to investigate the effect of these hormones on reproductive performance of Lake and Torki-Ghashghaei in rural breeding condition.
Materials and methods: This study was conducted in two separated experiments. In the first experiment 75 Lake-Ghashghaei ewes (3-4 years old with the mean weights of 542.6 kgs) and in the second experiment 75 Torki-Ghashghaei ewes (3-4 years old with the mean weights of 591.9 kgs) were used. The estrous cycle of ewes in breeding season was synchronized with progesterone sponges for a 14 days period. Then, one day before removing the sponges 500 and 600 IU of equine Chorionic Gonadotropin were injected intramuscularly to Lake-Ghashghaei and Torki-Ghashghaei ewes respectively. After removing the sponges all the ewes became cycled and divided into three different treatment groups. The first group in each experiment was assumed as control and the second and third groups received 500 IU human Chorionic Gonadotropine and 0.5 ml Gonadotropin Releasing Hormone respectively. Then, in each experiment parameters such as the time of estrus start (hour), rate of return to estrus, parturition rate, rate of multiple births number of lambs and rate of lambing were evaluated.
Results: The results in the first experiment (Lake-Ghashghaei ewes) indicated that the rate of return to estrus did not have significant differences among various treatment groups but the number of delivered ewes in human Chorionic Gonadotropine group was numerically more than other groups. The results also indicated that the number of single, twin and triple births' ewes was not significantly different among various treatment groups but the number of twin and triple births' ewes in human Chorionic Gonadotropine group was numerically more than other groups. The results in the second experiment (Torki-Ghashghaei ewes) indicated that the number of twin births' ewes fecundity and prolificacy rates were numerically more in human Chorionic Gonadotropine group compared with other groups. The results also indicated that the fecundity difference among different treatment groups had tendency to significantly (P=0.06) and this characteristic was higher in group receiving hCG, compared to other groups. Conclusion: According to the obtained results, it can be suggested that using human Chorionic Gonadotropine (500 IU) in superovulated Lake-Ghashghaei and Torki-Ghashghaei ewes with equine Chorionic Gonadotropin (500 and 600 IU, respectively) caused improvement the number of born lambs and fecundity rate.