شناسايي ژن‌هاي مؤثر بر ميزان چربي محوطۀ بطني جوجه‌هاي گوشتي با استفاده از داده‌هاي ريزآرايه و توالي‌يابي RNA

Identification of genes affecting the amount of abdominal fat in broiler chickens using microarray and RNA sequencing data

امروزه انتخاب ژنتيكي براي افزايش رشد و توليد پروتئين حيواني با كيفيت بالا در جوجه‌هاي گوشتي (Gallus gallus domesticus)، معمولاً منجر به افزايش وزن بيش از حد خواهد شد كه نتيجۀ آن تأثير منفي بر راندمان مصرف خوراك و كيفيت لاشه مانند افزايش چربي محوطه بطني مي­ شود. هدف اين مطالعه استفاده از پروفايل ترانسكريپتوم بافت چربي دو دسته جوجه‌هاي گوشتي با چربي زياد و كم در محوطۀ بطني، به‌منظور شناسايي ژن­ هاي مؤثر در متابوليسم و ذخيره چربي مي ­باشد. در تجزيه داده ­هاي ريزآرايه و RNA-seq براي بيان تفاوت ژني به­ ترتيب 2914 و 1867 ژن استخراج شد كه در مجموع با مقايسه ژن ­هاي شناسايي­ شده كه تفاوت بياني معني ­داري داشتند (0/000001

Abdominal fat deposition and several other unique features in the metabolism of birds such as interaction between genetic and endocrine factors, fasting hyperglycemia and insulin resistance are signs of obesity and metabolic disorders in poultry, similar to humans. The main purpose of this study was to use transcript profile of fat tissue in two groups of broiler chickens with high and low abdominal fat deposition, to identify the genes involved in storage and metabolism of fat, as well as the signaling pathways associated with the endocrine glands. Based on the analysis of microarray and RNA-seq data, 2914 and 1867 genes were detected as differentially expressed genes, respectively. In total, 1835 genes were identified by comparing the genes with a significant difference in expression (P<0.000001). Then, by comparing the number of relevant genes among the transcript profiles, the most important related genes were THBS1, COLEC12, ANXA7, RGS19, TMEM258 and HTR7L, which in the main process of pathways controlling synthesis, fat metabolism and storage and the endocrine signaling pathways activated by adipokines, are involved. The analysis of the relevant tissue may indicate the role of ventricular fat as a metabolic and endocrine organ in broiler chickens.