بررسي اثر ضد سرطاني چاي ترش، گلدر پرسيكا، گلدر اوشري وگلدر ميچاكسي بر رده سلولي اسكواموس سل كارسينوماي دهاني

Evaluation of anticancer effect of Hibiscus Sabdarifa, Otostegia Persica Otostegia Aucheri, and Otostegia Michauxii on oral squamous cell carcinoma cell line

زﻣﯿﻨﻪ و ﻫﺪف: اﺳﮑﻮاﻣﻮس ﺳﻞ ﮐﺎرﺳﯿﻨﻮﻣﺎي دﻫﺎﻧﯽ )Oral Squamous Cell Carcinoma, OSCC( ﯾﮑﯽ از ﺷﺎﯾﻊﺗﺮﯾﻦ ﺳﺮﻃﺎنﻫﺎي دﻫﺎن ﺑﺎ رﯾﺴﮏ اﻓﺰاﯾﺶ ﯾﺎﻓﺘﻪ در اﻓﺮاد ﻣﺴﻦﺗﺮ از 40 ﺳﺎل ﺑﻪ ﺧﺼﻮص در ﮐﺸﻮرﻫﺎي ﺗﻮﺳﻌﻪ ﯾﺎﻓﺘﻪ ﻣﯽﺑﺎﺷﺪ. ﺷﻮاﻫﺪ زﯾﺎدي وﺟﻮد دارد ﮐﻪ اﻓﺮادي ﮐﻪ از ﻣﯿﻮهﻫﺎ و ﺳﺒﺰﯾﺠﺎت ﺑﻪ ﻣﻘﺪار زﯾﺎدي در رژﯾﻢ ﻏﺬاﯾﯽ ﺧﻮد اﺳﺘﻔﺎده ﻣﯽﮐﻨﻨﺪ رﯾﺴﮏ ﮐﻤﺘﺮي ﻧﺴﺒﺖ ﺑﻪ ﺳﺎﯾﺮ اﻓﺮاد در اﺑﺘﻼ ﺑﻪ ﺳﺮﻃﺎنﻫﺎي ﻣﺎژور دارﻧﺪ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﺑﺮرﺳﯽ اﺛﺮ ﮐﺸﻨﺪﮔﯽ ﭼﻬﺎر ﮔﯿﺎه داروﯾﯽ ﮔﻠﺪر ﭘﺮﺳﯿﮑﺎ، ﮔﻠﺪر ﻣﯿﭽﺎﮐﺴﯽ، ﮔﻠﺪر اوﺷﺮي و ﭼﺎي ﺗﺮش ﺑﺮ روي رده ﺳﻠﻮﻟﯽ OSCC ﭘﺮداﺧﺘﯿﻢ. ﻫﺪف اﯾﻦ ﻣﻄﺎﻟﻌﻪ، ﺑﺮرﺳﯽ آزﻣﺎﯾﺸﮕﺎﻫﯽ اﺛﺮ ﺿﺪ ﺳﺮﻃﺎﻧﯽ ﭼﻬﺎر ﮔﯿﺎه داروﯾﯽ ﻓﻮق اﻟﺬﮐﺮ در ﻏﻠﻈﺖﻫﺎي ﻣﺘﻔﺎوت، روي رده ﺳﻠﻮﻟﯽ اﺳﮑﻮاﻣﻮس ﺳﻞ ﮐﺎرﺳﯿﻨﻮﻣﺎي دﻫﺎﻧﯽ ﺑﻮد. روش ﺑﺮرﺳﯽ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ از ﻋﺼﺎره ﻣﺘﺎﻧﻮﻟﯽ و دي ﮐﻠﺮو ﻣﺘﺎﻧﯽ ﭼﻬﺎرﮔﯿﺎه ﻓﻮق اﺳﺘﻔﺎده ﺷﺪ ﮐﻪ اﺛﺮ آنﻫﺎ ﺗﻮﺳﻂ ﺗﺴﺖ MTT )dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide-5 ,3-[4 ﺑﺮ روي رده ﺳﻠﻮﻟﯽ اﺳﮑﻮاﻣﻮس ﺳﻞ ﮐﺎرﺳﯿﻨﻮﻣﺎي دﻫﺎﻧﯽ در ﻏﻠﻈﺖﻫﺎي ﻣﺨﺘﻠﻒ و در زﻣﺎنﻫﺎي 24، 48 و 72 ﺳﺎﻋﺖ ﺑﺮرﺳﯽ ﮔﺮدﯾﺪ و ﻧﻬﺎﯾﺘﺎً ﻏﻠﻈﺖ ﻣﻨﺎﺳﺐ ﺟﻬﺖ ﺗﮑﺮار ﺗﺴﺖﻫﺎ اﻧﺘﺨﺎب ﮔﺮدﯾﺪ، ﺳﭙﺲ آﻧﺎﻟﯿﺰ دادهﻫﺎ ﺗﻮﺳﻂ ﺗﺴﺖ آﻣﺎري non parametric t test ﺻﻮرت ﮔﺮﻓﺖ. ﯾﺎﻓﺘﻪﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻣﯿﺰان IC50 ﻋﺼﺎره ﻣﺘﺎﻧﻮﻟﯽ O.Persica ﺑﻌﺪ از ﮔﺬﺷﺖ 24، 48 و 72 ﺳﺎﻋﺖ ﺑﻪ ﺗﺮﺗﯿﺐ 0/25، 0/12 و 0/13 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﻣﯿﻠﯽﻟﯿﺘﺮ ﻣﺤﺎﺳﺒﻪ ﺷﺪ. اﯾﻦ ﻣﻘﺎدﯾﺮ ﺑﺮاي ﻋﺼﺎره دي ﮐﻠﺮوﻣﺘﺎن اﯾﻦ ﮔﯿﺎه ﺑﻪ ﺗﺮﺗﯿﺐ mg/ml 0/17، 0/16 و 0/11 ﺑﻮد. ﻣﻘﺎدﯾﺮ IC50 ﻋﺼﺎره دي ﮐﻠﺮوﻣﺘﺎﻧﯽ O.Michauxii ﭘﺲ از ﮔﺬﺷﺖ 24، 48 و 72 ﺳﺎﻋﺖ ﺑﻪ ﺗﺮﺗﯿﺐ 0/73، 0/67 و 0/68 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻣﯿﻠﯽﻟﯿﺘﺮ ﻣﺤﺎﺳﺒﻪ ﺷﺪ. ﻋﺼﺎره ﻣﺘﺎﻧﻮﻟﯽ اﯾﻦ ﮔﯿﺎه ﺗﺄﺛﯿﺮي ﺑﺮ روي ﺳﻠﻮلﻫﺎ ﻧﺪاﺷﺖ و IC50 ﮔﺰارش ﻧﺸﺪ. ﭘﺲ از ﮔﺬﺷﺖ 24، 48 و 72 ﺳﺎﻋﺖ، ﻣﻘﺎدﯾﺮ IC50 ﺑﺮاي ﻋﺼﺎره ﻣﺘﺎﻧﻮﻟﯽ ﮔﯿﺎه O.Aucheri ﺑﻪ ﺗﺮﺗﯿﺐ mg/ml 0/59، 0/35 و 0/35 ﺑﻪ دﺳﺖ آﻣﺪ. اﯾﻦ ﻣﻘﺎدﯾﺮ ﺑﺮاي ﻋﺼﺎره دي ﮐﻠﺮوﻣﺘﺎن آن ﺑﻪ ﺗﺮﺗﯿﺐ 0/25، 0/20 و 0/15 mg/ml ﻣﺤﺎﺳﺒﻪ ﺷﺪ. ﻣﻘﺎدﯾﺮ IC50 ﻋﺼﺎره ﻣﺘﺎﻧﻮﻟﯽ ﮔﯿﺎه ﭼﺎي ﺗﺮش ﭘﺲ از ﮔﺬﺷﺖ 24، 48 و 72 ﺳﺎﻋﺖ ﺑﻪ ﺗﺮﺗﯿﺐ mg/ml 4/5، 3/56 و 2/55 ﺑﻮد. اﯾﻦ ﻣﻘﺎدﯾﺮ ﺑﺮاي ﻋﺼﺎره آﺑﯽ آن ﺑﻪ ﺗﺮﺗﯿﺐ mg/ml 7/9، 5/3 و 3/9 ﻣﺤﺎﺳﺒﻪ ﺷﺪ )0/05

Oral squamous cell carcinoma (OSCC) is one of the most prevalent oral cancers with increased risk in individuals younger than 40 years especially in developed countries. There are many evidences that people who have plenty of fruits and vegetables in their diets tend to have lower risk of cancer. In this study, we evaluated the anticancer effect of four medicinal plants of Otostegia Persica, Otostegia Michauxii, Otostegia Aucheri, and Hibiscus Sabdariffa on OSCC cell lines. Materials and Methods: In this study, methanolic & dichloromethane extracts of the above medicinal plants were used in order to evaluate their effect on oral squamous cell carcinoma cell line in different concentrations and after 24 , 48 and 72 hrs by MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay test. Finally, the appropriate concentration for repeating the test were chosen. Results: In this study, the mean amounts of IC50 for O.Persica and O.Aucheri were less than the two other herbs and the least and most amount of IC50 were related to the dicloromethanolic extract of O.Persica after 72 hrs and the water extract of H. sabdariffa after 24 hrs, respectively. Conclusion: It was concluded that all of the plants evaluated especially O.Persica and O.Aucheri had anticancer properties and dichloromethalonic extracts of these plant were more effective than that of methanolic extract.