اثر پيشگيرانة شش هفته تمرين شنا بر سطوح برخي از سايتوكاين‌ها و عامل تنظيم نوروني مشتق‌شده از مغز در رت‌هاي مدل EAE

Preventive effect six weeks of swimming training on the levels of cytokines and brain-derived neurotrophic rat model of EAE

ﻣﺎﻟﺘﯿﭙﻞ اﺳﮑﻠﺮوزﯾﺲ ﯾﮏ ﺑﯿﻤﺎري ﺧﻮداﯾﻤﻨﯽ ﭘﯿﺶروﻧﺪه و ﻣﺰﻣﻦ ﺳﯿﺴﺘﻢ اﻋﺼﺎب ﻣﺮﮐﺰي اﺳﺖ ﮐﻪ ﺑﺎ از ﺑﯿﻦ رﻓﺘﻦ ﻏﻼف ﻣﯿﻠﯿﻦ ﺳﻠﻮلﻫﺎي ﻋﺼﺒﯽ ﻫﻤﺮاه اﺳﺖ. ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، ﺑﺮرﺳﯽ اﺛﺮ ﭘﯿﺸﮕﯿﺮاﻧﮥ 6 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﺷﻨﺎي اﺟﺒﺎري ﺑﺮ ﺳﻄﻮح IL-10 ،TNF-α و BDNF در رتﻫﺎي ﻣﺪل EAE ﺑﻮد. 25 ﺳﺮ رت ﻣﺎدة ﻧﮋاد ﻟﻮﺋﯿﺲ ﺑﻪ ﭘﻨﺞ ﮔﺮوه ﺷﺎﻣﻞ ﮐﻨﺘﺮل ﺳﺎﻟﻢ، ﮐﻨﺘﺮل ام.اس، ﺷﻨﺎي ﺳﺎﻟﻢ، ﺷﻨﺎي ام.اس و ﺣﻼل ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﺗﻤﺮﯾﻨﺎت ﺷﻨﺎ ﺑﻪ ﻣﺪت 6 ﻫﻔﺘﻪ و 5 روز در ﻫﻔﺘﻪ اﻧﺠﺎم ﮔﺮﻓﺖ. ﺑﺮﻧﺎﻣﮥ ﺗﻤﺮﯾﻦ ﺷﻨﺎ ﺑﺎ 30 دﻗﯿﻘﻪ آﻏﺎز ﺷﺪ، ﮐﻪ اﯾﻦ ﻣﺪت ﺑﺎ اﻓﺰاﯾﺶ ﭘﻨﺞ دﻗﯿﻘﻪ روزاﻧﻪ در ﻫﻔﺘﮥ دوم ﺑﻪ 60 دﻗﯿﻘﻪ رﺳﯿﺪ. اﺿﺎﻓﻪﺑﺎر ﺗﻤﺮﯾﻨﯽ ﺑﺎ ﺗﻨﻈﯿﻢ ﻗﺪرت و ﺳﺮﻋﺖ آب ﻫﻨﮕﺎم ﺷﻨﺎ، ﺗﻨﻈﯿﻢ ﺷﺪ. ﻫﻤﭽﻨﯿﻦ، ﻣﺪل EAE در اﻧﺘﻬﺎي ﻫﻔﺘﮥ ﺷﺸﻢ ﺗﻤﺮﯾﻨﺎت اﻟﻘﺎ و ﭘﺲ از 14 روز ﺑﺎﻓﺖﺑﺮداري از ﻣﻐﺰ رتﻫﺎ اﻧﺠﺎم ﮔﺮﻓﺖ. ﻧﯿﻤﮑﺮة راﺳﺖ ﺑﺮاي ﺑﺎﻓﺖﺷﻨﺎﺳﯽ و ﻧﯿﻤﮑﺮة ﭼﭗ ﺑﻪﻣﻨﻈﻮر اﻧﺠﺎم آﻧﺎﻟﯿﺰ ﺳﻨﺠﺶ ﭘﺮوﺗﺌﯿﻦ ﺑﻪ آزﻣﺎﯾﺸﮕﺎه ﻣﻨﺘﻘﻞ ﺷﺪ. ﺗﻤﺮﯾﻦ ﺷﻨﺎ در رتﻫﺎي ﻣﺪل EAE ﺑﻪ ﮐﺎﻫﺶ ﻣﻌﻨﺎدار P < 0/001 TNF-α و ﻋﺪم ﺗﻐﯿﯿﺮ ﻣﻌﻨﺎداري در 10-P=0/71) IL( و P=0/70) BDNF( ﻣﻨﺠﺮ ﺷﺪ. ﻋﻼﺋﻢ ﺑﺎﻟﯿﻨﯽ در رتﻫﺎي ﺗﻤﺮﯾﻦﮐﺮده ﺑﺎ ﺗﺄﺧﯿﺮ ﻇﺎﻫﺮ ﺷﺪ. ﻋﻼوهﺑﺮ اﯾﻦ، ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از رﻧﮓآﻣﯿﺰي ﺑﺎﻓﺘﯽ ﻧﺸﺎن داد ﮐﻪ ﺑﯿﻦ ﮔﺮوه ﺗﻤﺮﯾﻦ ﮐﺮده و ﺗﻤﺮﯾﻦﻧﮑﺮده ﺗﻔﺎوت ﭼﺸﻤﮕﯿﺮي از ﻧﻈﺮ اﻟﺘﻬﺎب ﻣﺸﺎﻫﺪه ﻧﺸﺪ. دﻣﯿﻠﯿﻨﻪ ﺷﺪن ﻧﺎﺷﯽ از ﻣﺪل EAE ﺳﺒﺐ اﻓﺰاﯾﺶ آﺳﯿﺐ ﺑﻪ ﺑﺎﻓﺖ ﻣﻐﺰي ﻣﯽﺷﻮد ﮐﻪ ﻧﺸﺎن ﻣﯽدﻫﺪ ورزش ﺷﻨﺎ ﺑﺎ ﺷﺪت و ﻣﺪت ﻣﻄﺎﻟﻌﮥ ﺣﺎﺿﺮ ﻧﻤﯽﺗﻮاﻧﺪ در ﭘﯿﺸﮕﯿﺮي از ﺑﯿﻤﺎري ﻣﺆﺛﺮ ﺑﺎﺷﺪ، ﻫﺮﭼﻨﺪ اﯾﻦ ﻋﻼﺋﻢ در ﻣﺪت ﮐﻮﺗﺎه ﺑﻪ ﺗﺄﺧﯿﺮ اﻓﺘﺎده ﺑﺎﺷﺪ.

Multiple sclerosis is a progressive and chronic autoimmune disease of the central nervous system that is associated with the destruction of the myelin sheath of nerve cells. The aim of this study was to investigate the preventive effect of six weeks of forced swimming on the levels of TNF-α, IL-10 and BDNF is the EAE model rats. Twenty-five female Lewis were divided into five groups: healthy control, MS control, healthy swimming, MS swimming, and solvent. Swimming training for 6 weeks and 5 days a week. Swimming training program began with 30 minutes daily five-minute period in the second week with an increase to 60 minutes. Overload training was set by adjusting the power and speed of the water while swimming. Also, EAE was induced model at the end of the sixth week of training and After 14 days’ mice were sacrificed. Right Cerebral hemisphere for the histology and right Cerebral hemisphere was transferred to the lab for analysis of protein measurements. Swimming exercise in rats EAE model leads to a significant reduction in TNF-α (P=0/001) and no significant change in IL-10 (P=0/71) and BDNF (P=0/70) was. In rats trained clinical signs appeared delayed. Furthermore, results of histological staining showed that certain differences were observed between trained and untrained. EAE-induced demyelination model increases the damage to brain tissue Shows that swimming exercise intensity and duration of six weeks can be effective in delaying symptoms, although these symptoms are delayed in the short term.