تأثير هشت هفته تمرين تناوبي بر ميزان پروتئين‌هاي كلوتو، پروتئوليپيد پروتئين و فاكتور نكروزتوموري آلفا در بافت مخچة موش‌هاي سالم

The effect of 8 weeks of high-intensity interval training on Klotho, PLP and TNF-α Cerebellum tissue mice

اﻣﺮوزه در ﺗﺤﻘﯿﻘﺎت ﺑﺴﯿﺎري ﻧﺸﺎن داده ﺷﺪه اﺳﺖ ﮐﻪ ﺗﻤﺮﯾﻨﺎت ورزﺷﯽ ﺑﻪ ﺳﻼﻣﺖ و ﺑﻬﺒﻮد ﻋﻤﻠﮑﺮد ﻣﻐﺰ ﻣﻨﺠﺮ ﻣﯽﺷﻮد. ﻫﻤﭽﻨﯿﻦ ﻣﻤﮑﻦ اﺳﺖ ﻣﺴﯿﺮﻫﺎي ﻋﺼﺒﯽ و ﻧﻮروﻟﻮژﯾﮑﯽ را در ﻣﻐﺰ اﻓﺰاﯾﺶ دﻫﻨﺪ. ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﺑﺮرﺳﯽ ﺗﺄﺛﯿﺮ 8 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺑﺎ ﺷﺪت 80 درﺻﺪ ﺳﺮﻋﺖ ﺑﯿﺸﯿﻨﻪ در ﻫﻔﺘﮥ اول ﺗﺎ 110 درﺻﺪ ﺳﺮﻋﺖ ﺑﯿﺸﯿﻨﻪ در ﻫﻔﺘﮥ آﺧﺮ ﺑﺮ ﻣﯿﺰان ﮐﻠﻮﺗﻮ، PLPو TNF-α ﺑﺎﻓﺖ ﻣﺨﭽﮥ ﻣﻮشﻫﺎي ﺳﺎﻟﻢ ﺑﻮد. ﺑﺪﯾﻦﻣﻨﻈﻮر، ﺗﻌﺪاد 20 ﺳﺮ ﻣﻮش ﻣﺎده ﺑﻪﻃﻮر ﺗﺼﺎدﻓﯽ ﺑﻪ دو ﮔﺮوه ﺗﻤﺮﯾﻦ )10=n( و ﮐﻨﺘﺮل )10=n( ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. در ﮔﺮوه ﺗﻤﺮﯾﻦ، ﺣﯿﻮاﻧﺎت 8 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺑﺮ ﺗﺮدﻣﯿﻞ را اﺟﺮا ﮐﺮدﻧﺪ. اﺑﺘﺪا ﺑﻪ ﻣﺪت 3 دﻗﯿﻘﻪ ﺑﺎ ﺳﺮﻋﺖ 6 ﻣﺘﺮ ﺑﺮ دﻗﯿﻘﻪ دوﯾﺪﻧﺪ و ﻫﺮ 3 دﻗﯿﻘﻪ ﯾﮏﺑﺎر، 3 ﻣﺘﺮ ﺑﺮ دﻗﯿﻘﻪ ﺑﻪﺳﺮﻋﺖ ﺗﺮدﻣﯿﻞ اﺿﺎﻓﻪ ﺷﺪ ﺗﺎ زﻣﺎﻧﯽﮐﻪ ﻗﺎدر ﺑﻪ ﺣﻔﻆ اﯾﻦ ﺷﺪت ﻧﺒﺎﺷﻨﺪ. آﺧﺮﯾﻦ ﺗﻼش ﻫﺮ ﻣﻮش ﺑﻪﻋﻨﻮان ﺣﺪاﮐﺜﺮ ﺳﺮﻋﺖ درﻧﻈﺮ ﮔﺮﻓﺘﻪ ﺷﺪ. در ﻧﻬﺎﯾﺖ، ﭘﺮوﺗﮑﻞ ﺗﻤﺮﯾﻨﯽ ﺑﻪ ﻣﺪت 8 ﻫﻔﺘﻪ 5 ﺟﻠﺴﻪ در ﻫﻔﺘﻪ اﺟﺮا ﺷﺪ. از روش وﺳﺘﺮن ﺑﻼت ﺑﻪﻣﻨﻈﻮر اﻧﺪازهﮔﯿﺮي ﻣﺘﻐﯿﺮﻫﺎي ﺗﺤﻘﯿﻖ اﺳﺘﻔﺎده ﺷﺪ. دادهﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮن t ﻣﺴﺘﻘﻞ در ﺳﻄﺢ ﻣﻌﻨﺎداري 0/05 ≤P ﺗﺠﺰﯾﻪ ﺗﺤﻠﯿﻞ ﺷﺪﻧﺪ. ﻧﺘﺎﯾﺞ ﺗﺤﻘﯿﻖ ﻧﺸﺎن داد، ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺑﻪ اﻓﺰاﯾﺶ ﻣﯿﺰان ﮐﻠﻮﺗﻮ )0/01≤P≤0/05) PLP , (P( و ﮐﺎﻫﺶ ﻣﯿﺰان P≤0/05) TNF-α( در ﮔﺮوه ﺗﻤﺮﯾﻦ در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﮐﻨﺘﺮل ﻣﻨﺠﺮ ﺷﺪ. در ﻧﻬﺎﯾﺖ ﻣﯽﺗﻮان ﮔﻔﺖ 8 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﻣﯿﺰان ﭘﺮوﺗﺌﯿﻦﻫﺎي ﻣﯿﻠﯿﻦﺳﺎز را اﻓﺰاﯾﺶ و ﻣﯿﺰان TNF-α را ﮐﺎﻫﺶ داد. ﺑﻪﻧﻈﺮ ﻣﯽرﺳﺪ ﺗﻤﺮﯾﻦ ورزﺷﯽ ﺑﺎ اﻓﺰاﯾﺶ در ﺑﯿﻮﻣﺎرﮐﺮﻫﺎي ﻣﯿﻠﯿﻦﺳﺎز، ﻧﻘﺶ ﻣﻬﻤﯽ در ﺣﻔﺎﻇﺖ از ﺳﯿﺴﺘﻢ اﻋﺼﺎب ﻣﺮﮐﺰي دارد.

Nowadays, many studies have shown that exercise improves brain health and performance and may also increase neurological pathways in the brain. The aim of the present study was to investigate the effect of 8 weeks of high intensity interval training (HIIT) on Klotho, PLP and TNF-α concentrations in mice. Female mice were randomly divided to exercise (EX) or control (Con) groups. In the EX group, animals performed 8-week of HIIT. Mice initially ran at 6 m/min for 3 min at 0% grade, with the speed progressively increasing by 3 m/min every 3 min until exhaustion - when the mice were unable to maintain the required running speed. The maximal speed obtained was then used to calculate the individualized running speed for mice in the EX group. HIIT involved treadmill running 5 days per week for the final 6 weeks. Klotho, PLP and TNF-α expressions were studied using Western blot. Mice in the EX group had a significant increase in klotho and PLP concentrations compared to the control group (p≤0.01 and p≤0.05, respectively). Also, in the exercise groups mice had a significant decrease in TNF-α concentrations in compared to the control group (p≤0.05). Our data demonstrate that HIIT increased klotho and PLP and deceased neuroinflammatory cytokines. These proteins are associated with increasing myelination and protect the central nerve system against neurodegenerative diseases.